Abstract
The development of a transposon mutagenesis system in soybean would aid in the isolation of unknown genes. The maize controlling element (Ac) has, therefore, been introduced into the soybean (Glycine max (L.) Merr.) genome byAgrobacterium-mediated transformation.Ac was inserted into the untranslated leader region of the bacterial ß-glucuronidase gene (GUS) such that the excision ofAc resulted in restoration of the GUS gene activity. Excision events of theAc element were monitored by detecting blue cells or sectors in transgenic soybean tissues. Using the GUS gene assay and with hybridization data, we have demonstrated that theAc element transposes in transgenic soybean calli, leaves, stems, and roots.
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References
An G (1985) Plant Physiol 79:568–570.
An G (1986) Plant Physiol 81:86–91.
An G (1987) Methods in Enzymology 153:292–305
An G, Watson BD, Stachel S, Gordon MP, Nester EW (1985) EMBO J. 4:277–284.
Baker B, Schell J, Loerz H, Fedoroff N (1986) Proc Natl Acad Sci USA 83:4844–4848.
Baker B, Coupland G, Fedoroff N, Starlinger P, Schell J (1987) EMBO J 6:1547–1554.
Cone KC, Burr FA, Burr B (1986) Proc Natl Acad Sci USA 83:9631–9635.
Chen J, Greenblatt IM, Dellaporta SL (1987) Genetics 117:109–116.
Dellaporta SL, Wood J, Hicks JB (1983) Plant Mol Biol Rep 1:19–21.
Finnegan EJ, Taylor BH, Craig S, Dennis ES (1989) Plant Cell 1:757–764.
Fedoroff N, Furtek D, Nelson O (1984) Proc Natl Acad Sci USA 81:3825–3829.
Groose RW, Weigelt HD, Palmer RG (1988) J Hered 79:263–267.
Hake S, Vollbrecht E, Freeling M (1989) EMBO J 8:1522.
Hinchee MAW, Connor-Ward DV, Newell CA, McDonnell RE, Sato SJ, Gasser CS, Fischhoff DA, Re DB, Fraley RT, Horsch RB (1988) Bio/Technology 6:915–922.
Holsters M, de Waele D, de Picker A, Messens E, Van Montagu M, Schell J (1978) Mol Gen Genet 163:181–187.
Hood EE, Helmer GL, Fraley RT, Chilton M-D (1986) J Bacteriol 168:1291–1301
Jefferson RA (1987) Plant Mol Biol Rep 5:387–405.
Knapp S, Soupland G, Uhrig H, Starlinger P, Salamini F (1988) Mol Gen Genet 213:285–290.
Masson P, Fedoroff NV (1989) Proc Natl Acad Sci USA 86:2219–2223.
McCabe DE, Swain WF, Martinell BJ, Christou P (1988) Bio/Technology 6:923–926.
Mead DA, Szczesna-Skorupa E, Kemper B (1986) Protein Eng 1:67–74.
O'Reilly C, Shepherd NS, Pereira A, Schwarz-Sommer Zs, Bertram I, Robertson DS, Peterson PA, Saedler H (1985) EMBO J. 4:877–882.
Pereira A and Saedler H (1989) EMBO J. 8:1315–1321.
Rhodes PR, Vodkin LO (1985) Proc Natl Acad Sci USA 82:493–497.
Schmidt RJ, Burr FA, Burr B (1987) Science 238:960–963.
Van Sluys MA, Tempe J, Fedoroff N (1987) EMBO J 6:3881–3889.
Vodkin LO, Rhodes PR, Goldberg RB (1983) Cell 34:1023–1031.
Yoder J, Palys J, Alpert K, Lassner M (1988) Mol Gen Genet 213:291–296.
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Communicated by J. M. Widholm
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Zhou, J.H., Atherly, A.G. In situ detection of transposition of the maize controlling element (Ac) in transgenic soybean tissues. Plant Cell Reports 8, 542–545 (1990). https://doi.org/10.1007/BF00820205
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DOI: https://doi.org/10.1007/BF00820205