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Molecular cloning, functional characterization and expression analysis of a novel monosaccharide transporter gene OsMST6 from rice (Oryza sativa L.)

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Abstract

Monosaccharides transporters play important roles in assimilate supply for sink tissue development. In this study, a new monosaccharide transporter gene OsMST6 was identified from rice (Oryza sativa L.). The predicted OsMST6 protein shows typical features of sugar transporters and shares 79.6% identity with the rice monosaccharide transporter OsMST3. Heterologous expression in yeast (Saccharomyces cerevisiae) demonstrated that OsMST6 is a broad-spectrum monosaccharide transporter, with a K m of 266.1 μΜ for glucose. OsMST6-green fluorescent protein fusion protein is localized to the plasma membrane in plant. Semi-quantitative RT-PCR analysis exhibited that OsMST6 is expressed in all tested organs/tissues. In developing seeds, OsMST6 expression level is high at the early and middle grain filling stages and gradually declines later. Further analysis detected its expression in both maternal and filial tissues. RNA in situ hybridization analysis indicated that OsMST6 is predominantly expressed in the vascular parenchyma of the chalazal vein, cross-cells, nucellar tissue and endosperm of young seeds, in mesophyll cells of source leaf blades, and in pollens and the connective vein of anthers. In addition, OsMST6 expression is up-regulated by salt stress and sugars. The physiological role of OsMST6 for seed development and its roles in other sink and source tissues are discussed.

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Abbreviations

Contig:

Contiguous DNA sequence

CSLM:

Confocal scanning laser microscopy

CWI:

Cell-wall invertase

DAF:

Day(s) after flowering

DIG:

Digoxigenin

MST:

Monosaccharide transporter

SUT:

Sucrose transporter

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Acknowledgments

We thank Dr. Eckhard Boles (Heinrich-Heine-Universität, Düsseldorf, Germany) for providing the S. cerevisiae strain EBY.VW4000 and Dr. Michael Büttner (Universität Erlangen-Nürnberg, Erlangen, Germany) for providing the S. cerevisiae/E. coli shuttle vector pEX-Tag. This work is supported by grants from the National Hi-tech Research and Development Program (2006AA10A101), the Knowledge Innovation Program of Chinese Academy of Science (KSCX1-YW-03), the Programme for Strategic Scientific Alliances (04-PSA-BD-04) between China and the Netherlands of the Royal Netherlands Academy of Arts and Sciences (KNAW) for P.B.F.O. and M.W., and the KNAW China Exchange Programme (04CDP022) for Y.X. and the CAS KNAW Joint PhD Training Programme (05-PhD-02) for Y.Z.

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Authors and Affiliations

  1. State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, 100101, Beijing, China

    Yongqin Wang, Yuguo Xiao, Yu Zhang, Chenglin Chai, Gang Wei, Xiaoli Wei, Honglin Xu & Zhen Zhu

  2. Department of Molecular and Developmental Genetics, Institute of Biology (IBL), Leiden University, P.O. Box 9505, 2300RA, Leiden, The Netherlands

    Yuguo Xiao, Yu Zhang, Mei Wang & Pieter B. F. Ouwerkerk

  3. TNO Quality of Life, Zernikedreef 9, 2333CK, Leiden, The Netherlands

    Mei Wang

  4. Graduate School of the Chinese Academy of Sciences, 100049, Beijing, China

    Yongqin Wang, Yuguo Xiao, Yu Zhang, Chenglin Chai & Gang Wei

Authors
  1. Yongqin Wang
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  2. Yuguo Xiao
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  3. Yu Zhang
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  4. Chenglin Chai
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  5. Gang Wei
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  6. Xiaoli Wei
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  7. Honglin Xu
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  8. Mei Wang
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  9. Pieter B. F. Ouwerkerk
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  10. Zhen Zhu
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Corresponding author

Correspondence to Zhen Zhu.

Additional information

Accession number: The nucleotide sequence of OsMST6 cDNA has been submitted to GenBank under the accession number AY342322.

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Wang, Y., Xiao, Y., Zhang, Y. et al. Molecular cloning, functional characterization and expression analysis of a novel monosaccharide transporter gene OsMST6 from rice (Oryza sativa L.). Planta 228, 525–535 (2008). https://doi.org/10.1007/s00425-008-0755-8

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  • DOI: https://doi.org/10.1007/s00425-008-0755-8

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