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Wounding and pathogen infection induce a chloroplast-targeted lipoxygenase in the common bean (Phaseolus vulgaris L.)

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Abstract

Chloroplastic LOXs are implicated in the biosynthesis of oxylipins like jasmonic acid and C6 volatiles among others. In this study, we isolated the cDNA of a novel chloroplast-targeted Phaseolus vulgaris LOX, (PvLOX6). This gene is highly induced after wounding, non-host pathogen infection, and by signaling molecules as H2O2, SA, ethylene and MeJA. The phylogenetic analysis of PvLOX6 showed that it is closely related to chloroplast-targeted LOX from potato (H1) and tomato (TomLOXC); both of them are implicated in the biosynthesis of C6 volatiles. Induction of PvLOX6 mRNA by wounding ethylene and jasmonic acid on the one side, and non-host pathogen, salicylic acid on the other indicates that common bean uses the same LOX to synthesize oxylipins in response to different stresses.

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Abbreviations

AOS:

Allene oxide synthase

HPL:

Hydroperoxide lyase

JA:

Jasmonic acid

LOX:

Lipoxygenase

MeJA:

Methyl jasmonate

PvLOX6:

Phaseolus vulgaris lipoxygenase 6

OPDA:

12-oxo-phytodienoic acid

SA:

Salicylic acid

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Acknowledgments

We thank Patricia Rueda for the technical support, Eugenio López-Bustos and Paul Gaytán for the oligonucleotide synthesis, Jorge Yanez for the sequencing, Andrés Saralegui for the confocal images, and José J. Sánchez Serrano from CNB, Madrid, Spain, for providing us with the potato H1 antibodies. We also thank Gladys Cassab and Miguel Angel Cevallos for their helpful comments on the manuscript. The Dirección General de Asuntos Para el Personal Académico-UNAM (IN212103) funded for this work.

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Authors and Affiliations

  1. Departmento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Cuernavaca, Morelos, C. P. 6100, Mexico

    Helena Porta, Rosa Elia Figueroa-Balderas & Mario Rocha-Sosa

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  1. Helena Porta
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  2. Rosa Elia Figueroa-Balderas
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  3. Mario Rocha-Sosa
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Correspondence to Mario Rocha-Sosa.

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PvLOX6 accession number: EF196866.

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Porta, H., Figueroa-Balderas, R.E. & Rocha-Sosa, M. Wounding and pathogen infection induce a chloroplast-targeted lipoxygenase in the common bean (Phaseolus vulgaris L.). Planta 227, 363–373 (2008). https://doi.org/10.1007/s00425-007-0623-y

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