Abstract
Lonicera macranthoides Hand-Mazz contains high levels of chlorogenic acid (CGA). The CGA is synthesized via different biosynthetic pathways in various plant species, and hydroxycinnamoyl-coenzyme A quinate transferases (HQTs) are key enzymes in these routes. In this study, we isolated the LmHQT1 gene, which encodes a protein of 447 amino acid residues with conserved HXXXD and DFGWG motifs. It is very closely homologous to HQT genes in Lonicera japonica (LjHQT), Solanum lycopersicum (SlHQT) and Nicotiana sylvestris (NsHQT). Quantitative reverse-transcription polymerase chain reaction showed that LmHQT1 gene expression decreased following leaf senescence. The CGA contents displayed similar trends, suggesting a potential role of LmHQT1 in CGA biosynthesis. To characterize its function, LmHQT1 overexpressing plants were generated via Agrobacterium transformation methods established previously. Upregulation of LmHQT1 in L. macranthoides was observed to elevate the CGA levels up to 60% in leaves. These findings indicated that LmHQT1was devoted to CGA biosynthesis in L. macranthoides.
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Acknowledgements
This work was supported by the Scientific and Technological Research Program of Chongqing Municipal Education Commission (Project No. KJ1401103), Chongqing Natural Science Foundation (Project No. cstc2012jjA80018, cstc2014jcyjA80035), the National Natural Science Foundation of China (Grant No. 31200512), and the Scientific Research Foundation of Chongqing University of Arts and Science of China (Grant No. Z2011RCYJ07).
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Communicated by J. Gao.
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Chen, Z., Liu, G., Liu, Y. et al. Overexpression of the LmHQT1 gene increases chlorogenic acid production in Lonicera macranthoides Hand-Mazz. Acta Physiol Plant 39, 27 (2017). https://doi.org/10.1007/s11738-016-2310-8
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DOI: https://doi.org/10.1007/s11738-016-2310-8