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Hydrogen peroxide generated by over-expression of cytosolic superoxide dismutase in transgenic plums enhances bacterial canker resistance and modulates plant defence responses

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Abstract

H2O2 generated during the oxidative burst, plays important roles in plant defenses responses against pathogens. In this study we examined the role of H2O2 on bacterial canker resistance in transgenic plums over-expressing cytosolic superoxide dismutase. Three transgenic lines (C64, C66 and F12) with elevated levels of H2O2 accumulation showed enhanced resistance against bacterial canker disease caused by Pseudomonas syringae pv. syringae, when compared to the non-transformed control. Analysis of the expression of several genes involved in the plant–pathogen interaction showed that the expression of those involved in SA pathway (pr1 and npr1) and JA (lox3) were activated earlier and transiently in transgenic lines C66 and F12 when compared to the wild type. However, the expression of genes involved in anthocyanin synthesis (chi, chs, f3h, dfr, atcs, myb10) and ethylene (acs) was induced at very low levels whereas it was activated by the pathogen at exaggerated levels in the non-transformed line. These results suggest that resistance observed in transgenic lines over-producing H2O2 is correlated with an early and transient induction of defense genes associated with the SA and JA pathways and inhibition of gene expression associated with ethylene and anthocyanin biosynthesis.

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Acknowledgements

This work was supported by the Spanish Ministry and Competitiveness (Project CICYT BFU2009-07443). MF was supported by the University Chouaib Doukkali.

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Authors and Affiliations

  1. Laboratory of Plant Biotechnology, Ecology and Ecosystem Valorisation, Faculty of Sciences, University Chouaib Doukkali, 24000, El Jadida, Morocco

    Mohamed Faize

  2. Group of Fruit Tree Biotechnology, Department of Plant Breeding, CEBAS-CSIC, PO Box 164, 30100, Murcia, Spain

    Lydia Faize, Nuria Alburquerque & Lorenzo Burgos

  3. PIAF-UMR547, University Blaise Pascal, 63177, Aubierre, France

    Jean Stéphane Venisse

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Contributions

MF designed the research, performed the experiments and wrote the manuscript. LF carried out the experiments, prepared tables and figures and participated in data analyses. JSV participated in data analysis and manuscript writing. NA and LB contributed to the transformation experiments and performed statistical analyses.

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Correspondence to Mohamed Faize.

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Faize, M., Faize, L., Alburquerque, N. et al. Hydrogen peroxide generated by over-expression of cytosolic superoxide dismutase in transgenic plums enhances bacterial canker resistance and modulates plant defence responses. Mol Biol Rep 47, 5889–5901 (2020). https://doi.org/10.1007/s11033-020-05660-8

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  • DOI: https://doi.org/10.1007/s11033-020-05660-8

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