Abstract
Sex determination for unidentified human remains is important because the determined sex is usually used as a foundation for the estimation of other biological profiles. This study investigated the sexual dimorphism of the adult hyoid bone and developed discriminant equations to accurately estimate sex in a contemporary Japanese population using measurements on two-dimensional computed tomography (CT) images. The study sample comprised 280 cadavers (140 males, 140 females) of known age and sex that underwent postmortem CT and subsequent forensic autopsy. In accordance with previous studies, seven measurements were performed using two-dimensional CT reconstructed images. Most measurements of males were significantly greater than those of females. The perpendicular length from the most anterior edge of the hyoid body to the line that connects the most distal points of the greater horns of the hyoid and the linear distance between the most lateral edges of the hyoid body most significantly contributed to sex determination. This study demonstrated that discriminant functions on the basis of three measurements provided higher rates of accurate sex classification (93.3–94.6%) than univariate functions. Therefore, the hyoid bone is highly sexually dimorphic in the contemporary Japanese population and may be useful in forensic contexts for sex determination with a high level of accuracy.
Similar content being viewed by others
References
Balseven-Odabasi A, Yalcinozan E, Keten A, Akçan R, Tumer AR, Onan A, Canturk N, Odabasi O, Hakan Dinc A (2013) Age and sex estimation by metric measurements and fusion of hyoid bone in a Turkish population. J Forensic Legal Med 20:496–501
Franklin D, Cardini A, Flavel A, Marks MK (2014) Morphometric analysis of pelvic sexual dimorphism in a contemporary western Australian population. Int J Legal Med 128:861–872
Özer I, Katayama K, Sağir M, Güleç E (2006) Sex determination using the scapula in medieval skeletons from east Anatolia. Coll Antropol 30:415–419
Scheuer L, Black S (2000) Developmental juvenile osteology. Elsevier ⁄ Academic Press, Amsterdam
Krogman WM, Iscan MY (1986) The human skeleton in forensic medicine. Charles C. Thomas, Springfield
Logar CJ, Peckmann TR, Meek S, Walls SG (2016) Determination of sex from the hyoid bone in a contemporary white population. J Forensic Legal Med 39:34–41
Khokhlov VD (1997) Injuries to the hyoid bone and laryngeal cartilages: effectiveness of different methods of medico-legal investigation. Forensic Sci Int 88:173–183
Maxeiner H (1998) “Hidden” laryngeal injuries in homicidal strangulation: how to detect and interpret these findings. J Forensic Sci 43:784–791
Jiménez-Brobeil SA, Al Oumaoui I, Fernández de la Gala JV, Laffranchi Z, Roca MG (2011) An example of a severe neck injury with survival seen in a bronze age burial. Int J Osteoarchaeol 21:247–252
Komenda S, Černý M (1990) Sex determination from the hyoid bone by means of discriminant analysis. Acta Univ Palacki Fac Med 125:37–51
Kim DI, Lee UY, Park DK, Kim YS, Han KH, Kim KH, Han SH (2006) Morphometrics of the hyoid bone for human sex determination from digital photographs. J Forensic Sci 51:979–984
Green H, Curnoe D (2009) Sexual dimorphism in south east Asian crania: a geometric morphometric approach. Homo 60:517–534
Soltani S, Aghakhani K, Fallah F (2017) Sex prediction potential of hyoid metric measurements in Iranian adults. Leg Med (Tokyo) 25:6–10
D'Anastasio R, Viciano J, Di Nicola M, Cesana DT, Sciubba M, Del Cimmuto M, Paolucci A, Fazio A, Capasso L (2014) Estimation of sex from the hyoid body in skeletal individuals from archeological sites. Homo 65:311–321
Fakhry N, Puymerail L, Michel J, Santini L, Lebreton-Chakour C, Robert D, Giovanni A, Adalian P, Dessi P (2013) Analysis of hyoid bone using 3D geometric morphometrics: an anatomical study and discussion of potential clinical implications. Dysphagia 28:435–445
Urbanová P, Hejna P, Zátopková L, Šafr M (2013) What is the appropriate approach in sex determination of hyoid bones? J Forensic Legal Med 20:996–1003
Torimitsu S, Makino Y, Saitoh H, Sakuma A, Ishii N, Hayakawa M, Yajima D, Inokuchi G, Motomura A, Chiba F, Iwase H (2015) Stature estimation in Japanese cadavers based on pelvic measurements in three-dimensional multidetector computed tomographic images. Int J Legal Med 129:633–639
Thali MJ, Braun M, Buck U, Aghayev E, Jackowski C, Vock P, Sonnenschein M, Dirnhofer R (2005) VIRTOPSY—scientific documentation, reconstruction and animation in forensic: individual and real 3D data based geo-metric approach including optical body/object surface and radiological CT/MRI scanning. J Forensic Sci 50:428–442
Dedouit F, Telmon N, Costagliola R, Otal P, Joffre F, Rougé D (2007) Virtual anthropology and forensic identification: report of one case. Forensic Sci Int 173:182–187
Ramsthaler F, Kettner M, Gehl A, Verhoff MA (2010) Digital forensic osteology: morphological sexing of skeletal remains using volume-rendered cranial CT scans. Forensic Sci Int 195:148–152
Robinson C, Eisma R, Morgan B, Jeffery A, Graham E, Black S, Rutty GN (2008) Anthropological measurement of lower limb and foot bones using multidetector computed tomography. J Forensic Sci 53:1289–1295
Torimitsu S, Makino Y, Saitoh H, Sakuma A, Ishii N, Inokuchi G, Motomura A, Chiba F, Hoshioka Y, Iwase H (2015) Estimation of sex in Japanese cadavers based on sternal measurements using multidetector computed tomography. Leg Med (Tokyo) 17:226–231
Franklin D, Cardini A, Flavel A, Kuliukas A (2012) Linear measurements and geometric morphometric data for quantifying cranial sexual dimorphism: preliminary investigations in a western Australian population. Int J Legal Med 126:549–558
Kindschuh SC, Dupras TL, Cowgill LW (2010) Determination of sex from the hyoid bone. Am J Phys Anthropol 143:279–284
Miller KWP, Walker PL, O'Halloran RL (1998) Age and sex related variation in hyoid bone morphology. J Forensic Sci 43:1138–1143
Ward RE, Jamison PL (1991) Measurement precision and reliability in craniofacial anthropometry: implications and suggestions for clinical applications. J Craniofac Genet Dev Biol 11:156–164
Weinberg SM, Scott NM, Neiswanger K, Marazita ML (2005) Intraobserver error associated with measurements of the hand. Am J Hum Biol 17:368–371
Bass WM (2005) Human osteology: a laboratory and field manual, 5th edn. Missouri Archaeological Society, University of Columbia
Mahakkanukrauh P, Sinthubua A, Prasitwattanaseree S, Ruengdit S, Singsuwan P, Praneatpolgrang S, Duangto P (2015) Craniometric study for sex determination in a Thai population. Anat Cell Biol 48:275–283
Manchanda AS, Narang RS, Kahlon SS, Singh B (2015) Diagonal tooth measurements in sex assessment: a study on north Indian population. J Forensic Dent Sci 7:126–131
Gupta A, Kohli A, Aggarwai NK, Banerjee KK (2008) Study of age of fusion of hyoid bone. Leg Med (Tokyo) 10:253–256
Shimizu Y, Kanetaka H, Kim YH, Okayama K, Kano M, Kikuchi M (2005) Age related morphological changes in human hyoid bone. Cells Tissues Organs 180:185–192
O’Halloran RL, Lundy JK (1987) Age and ossification of the hyoid bone: forensic implications. J Forensic Sci 32:1655–1659
Loth A, Corny J, Santini L, Dahan L, Dessi P, Adalian P, Fakhry N (2015) Analysis of hyoid-larynx complex using 3D geometric morphometrics. Dysphagia 30:357–364
Urbanová P, Hejna P, Zátopková L, Šafr M (2013) The morphology of human hyoid bone in relation to sex, age and body proportions. Homo 64:190–204
Pollard J, Piercecchi-Marti MD, Thollon L, Bartoli C, Adalian P, Bécart-Robert A, Tournel G, Hédouin V, Panuel M, Gosset D, Leonetti G (2011) Mechanisms of hyoid bone fracture after modelling: evaluation of anthropological criteria defining two relevant models. Forensic Sci Int 212:274.e1–274.e5
Mukhopadhyay PP (2010) Morphometric features and sexual dimorphism of adult hyoid bone: a population specific study with forensic implications. J Forensic Legal Med 17:321–324
Colman KL, Dobbe JG, Stull KE, Ruijter JM, Oostra RJ, van Rijn RR, van der Merwe AE, de Boer HH, Streekstra GJ (2017) The geometrical precision of virtual bone models derived from clinical computed tomography data for forensic anthropology. Int J Legal Med 131:1155–1163
Torimitsu S, Makino Y, Saitoh H, Sakuma A, Ishii N, Yajima D, Inokuchi G, Motomura A, Chiba F, Yamaguchi R, Hashimoto M, Hoshioka Y, Iwase H (2016) Sexual determination based on multidetector computed tomographic measurements of the second cervical vertebra in a contemporary Japanese population. Forensic Sci Int 266:588.e1–588.e6
Torimitsu S, Makino Y, Saitoh H, Sakuma A, Ishii N, Yajima D, Inokuchi G, Motomura A, Chiba F, Yamaguchi R, Hashimoto M, Hoshioka Y, Iwase H (2016) Sex determination based on sacral and coccygeal measurements using multidetector computed tomography in a contemporary Japanese population. J Forensic Radiol Imaging 9:8–12
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflicts of interest.
Ethical standards
This study complies with the current laws of the country in which it was performed.
Rights and permissions
About this article
Cite this article
Torimitsu, S., Makino, Y., Saitoh, H. et al. Determination of sex on the basis of hyoid bone measurements in a Japanese population using multidetector computed tomography. Int J Legal Med 132, 907–914 (2018). https://doi.org/10.1007/s00414-017-1728-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00414-017-1728-x