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Expression of matrix metalloproteinases (MMP-2 and MMP-9) in recurrent respiratory papillomas and laryngeal carcinoma: clinical and morphological parallels

  • Laryngology
  • Published:
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Abstract

The task of the present study was to investigate the expression of MMP-2 and MMP-9 in recurrent respiratory papillomas (RRP) and accomplish a comparative analysis with those in laryngeal squamous cell carcinoma (LSCC). The immunohistochemical expression of MMP-2 and MMP-9 was investigated in specimens taken from RRP (n = 38) and LSCC (n = 39) patient groups, and the normal tissue of vocal fold (n = 12, control group). The expression of MMP-2 and MMP-9, both in epithelium and stroma cells, was graded on a semiquantitative scale, ranging from 0 (no expression) to 18 points (high expression). Statistically significant differences in the expression of MMP-2 and MMP-9, both in epithelium and stroma among the RRP, LSCC patients and control group (epithelium) with the LSCC group having the highest MMPs expression scores were revealed. However, no statistically significant correlations among expression of MMPs and clinical and/or morphological features were found in the group of RRP patients. The MMP-2 stroma value of 10.4 points was determined as the optimum point (limiting value) for separating RRP and LSCC patient groups. Results of the present study indicate that the expression of both MMP-2 and MMP-9 are up-regulated early in development of laryngeal papillomas, when the benign neoplastic lesion begins and the next determinant step is concerned with the occurrence of malignization. These results seem promising, as they may improve our understanding of the molecular events leading to the papilloma formation and development, however, further research is needed.

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References

  1. Wiatrak B (2003) Overview of recurrent papillomatosis. Curr Opin Otolaryngol Head Neck Surg 11:433–941

    Article  PubMed  Google Scholar 

  2. Uloza V (2000) The course of laryngeal papillomatosis treated by endolaryngeal microsurgery. Eur Arch Otorhinolaryngol 257:498–501

    Article  PubMed  CAS  Google Scholar 

  3. Hobbs CGL, Birchall MA (2004) Human papillomavirus infection in the etiology of laryngeal carcinoma. Otolaryngol Head Neck Surg 12:88–92

    Google Scholar 

  4. Derkay C (1995) Task force on recurrent respiratory papillomas. A preliminary report. Arch Otolaryngol Head Neck Surg 121:1386–1391

    PubMed  CAS  Google Scholar 

  5. Goon P, Sonnex C, Jani P, Stanley M, Sudhoff H (2008) Reccurent respiratory papillomatosis: an overview of current thinking and treatment. Eur Arch Otorhinolaryngol 265:147–151

    Article  PubMed  Google Scholar 

  6. Campisi P, Hawkes M, Simpson K (2010) The epidemiology of juvenile onset recurrent respiratory papillomatosis derived from a population level national database. Laryngoscope 120:1233–1245

    PubMed  Google Scholar 

  7. Larson DA, Derkay CS (2010) Epidemiology of recurrent respiratory papillomatosis. APMIS 118:450–454

    Article  PubMed  Google Scholar 

  8. Glynn M, Sanford T, Hoover L, Kinsey W, Dobbs L, Bruegger D (1999) Characterization of human papillomavirus in airway papillomas by histologic and biochemical analysis. Ann Otol Rhinol Laryngol 108:1073–1077

    PubMed  CAS  Google Scholar 

  9. Velyvyte D, Laiskonis A, Uloza V, Gozdzicka-Jozefiak A (2002) Prevalence of papillomavirus infection among patients with laryngeal papillomatosis and the effects of some risk factors on the persistence of papillomaviruses in the upper respiratory tract. Medicina 38:499–504

    PubMed  Google Scholar 

  10. Xue Q, Wang H, Wang J (2010) Recurrent respiratory papillomatosis: an overview. Eur J Clin Microbiol Infect Dis 29:1051–1054

    Article  PubMed  Google Scholar 

  11. Mammas IN, Sourvinos G, Vakonaki E, Giamarelou P, Michael C, Spandidos DA (2010) Novel human papilloma virus (HPV) genotypes in children with recurrent respiratory papillomatosis. Eur J Pediatr 169:1017–1021

    Article  PubMed  Google Scholar 

  12. Vambutas A, Di Lorenzo TP, Steinberg BM (1993) Laryngeal papillomacells have high levels of epidermal growth factor receptor and respond to epidermal growth factor by a decrease in epithelial differentiation. Cancer Res 53:910–914

    PubMed  CAS  Google Scholar 

  13. Derkay CS, Wiatrak B (2008) Recurrent respiratory papillomatosis: a review. Laryngoscope 118:1236–1247

    Article  PubMed  Google Scholar 

  14. Gallagher TQ, Derkay CS (2008) Recurrent respiratory papillomatosis: update 2008. Curr Opin Otolaryngol Head Neck Surg 16:536–542

    Article  PubMed  Google Scholar 

  15. Lin HW, Richmon JD, Emerick KS, de Venecia RK, Zeitels SM, Faquin WC, Lin DT (2010) Malignant transformation of a highly aggressive human papillomavirus type 11–associated recurrent respiratory papillomatosis. Am J Otolaryngol 31:291–296

    Article  PubMed  CAS  Google Scholar 

  16. Baumann JL, Cohen S, Evjen AN, Law JH, Vadivelu S, Attia A, Schindler JS, Chung CH, Wirth PS, Meijer CJ, Snijders PJ, Yarbrough WG, Slebos RJ (2009) Human papillomavirus in early laryngeal carcinoma. Laryngoscope 119:1531–1537

    Article  PubMed  Google Scholar 

  17. Torrente MC, Rodrigo JP, Haigentz M, Dikkers FG, Rinaldo A, Takes RP, Olofsson J, Ferlito A (2010) Human Papillomavirus Infections in Laryngeal Cancer. Head and Neck 2010. [Epub ahead of print]

  18. Rady PL, Schnading VJ, Weiss RL, Hughes TK, Tyring SK (1998) Malignant transformation of reccurent respiratory papillomatosis associated with integrated human papillomavirus type 11 DNA and mutation of p53. Laryngoscope 108:735–740

    Article  PubMed  CAS  Google Scholar 

  19. Nadal A, Cardesa A (2003) Molecular biology of laryngeal squamous cell carcinoma. Virchows Arch 442:1–7

    PubMed  CAS  Google Scholar 

  20. Bolontrade MF, Stern MC, Binder RL, Zenklusen JC, Gimenez-Conti IB, Conti CJ (1998) Angiogenesis is an early event in the development of chemically induced skin tumors. Carcinogenesis 19:2107–2113

    Article  PubMed  CAS  Google Scholar 

  21. Weeks BH, He W, Olson KL, Wang XJ (2001) Inducible expression of transforming growth factor beta1 in papillomas causes rapid metastasis. Cancer Res 61:7435–7443

    PubMed  CAS  Google Scholar 

  22. Nelson AR, Fingleton B, Rothenberg ML, Matrisian LM (2000) Matrix metalloproteinases: biologic activity and clinical implications. J Clin Oncol 18:1135–1149

    PubMed  CAS  Google Scholar 

  23. Vihinen P, Kähäri VM (2002) Matrix metalloproteinases in cancer: prognostic markers and therapeutic targets. Int J Cancer 99:157–166

    Article  PubMed  CAS  Google Scholar 

  24. Bogusiewicz M, Stryjecka-Zimmer M, Szymanski M, Rechberger T, Golabek W (2003) Activity of matrix metalloproteinases-2 and -9 in advanced laryngeal cancer. Otolaryngol Head Neck Surg 128:132–136

    Article  PubMed  Google Scholar 

  25. Liu WW, Zeng ZY, Wu QL, Hou JH, Chen YY (2005) Overexpression of MMP-2 in laryngeal squamous cell carcinoma: a potential indicator for poor prognosis. Otolaryngol Head Neck Surg 132:395–400

    Article  PubMed  Google Scholar 

  26. Christopoulos TA, Papageorgakopoulou N, Ravazoula P, Mastronikolis NS, Papadas TA, Theocharis DA, Vynios DH (2007) Expression of metalloproteinases and their tissue inhibitors in squamous cell laryngeal carcinoma. Oncol Rep 18:855–860

    PubMed  CAS  Google Scholar 

  27. Zhang H, Liu M, Sun Y, Lu J (2009) MMP-14 can serve as a prognostic marker in patients with supraglottic cancer. Eur Arch Otorhinolaryngol 266:1427–1434

    Article  PubMed  Google Scholar 

  28. Sarioğlu S, Ozer E, Kirimca F, Sis B, Pabuçcuoğlu U (2001) Matrix metalloproteinase-2 expression in laryngeal preneoplastic and neoplastic lesions. Pathol Res Pract 197:483–486

    Article  PubMed  Google Scholar 

  29. Lesauskaite V, Uloza V, Liutkevicius V, Pangonyte D (2008) Expression of matrix metalloproteinases (MMP-2 and MMP-9) in vocal fold polyps. Medicina (Kaunas) 44:322–327

    Google Scholar 

  30. Karahan N, Baspinar S, Yariktas M, Kapucuoglu N (2009) Matrix metalloproteinases (MMP-2 and MMP-9) and cyclooxygenase- 2 (COX-2) expressions in vocal cord polyps. J Voice 23:29–33

    Article  PubMed  Google Scholar 

  31. Peschos D, Damala C, Stefanou D, Tsanou E, Assimakopoulos D, Vougiouklakis T, Charalabopoulos K, Agnantis NJ (2006) Expression of matrix metalloproteinase-9 (gelatinase B) in benign, premalignant and malignant laryngeal lesions. Histol Histopathol 21:603–608

    PubMed  CAS  Google Scholar 

  32. Dikkers FG (2006) Treatment of recurrent respiratory papillomatosis with microsurgery in combination with intralesional cidofovir—a prospective study. Eur Arch Otorhinolaryngol 263:440–443

    Article  PubMed  Google Scholar 

  33. Sajan JA, Kerschner JE, Merati AL, Osipov V, Szabo S, Blumin JH (2010) Prevalence of dysplasia in juvenile-onset recurrent respiratory papillomatosis. Arch Otolaryngol Head Neck Surg 136:7–11

    Article  PubMed  Google Scholar 

  34. Bonagura VR, Hatam LJ, Rosenthal DW, DeVoti JA, Lam F, Steinberg BM, Abramson AL (2010) Recurrent respiratory papillomatosis: a complex defect in immune responsiveness to human papillomavirus-6 and -11. APMIS 118:455–470

    Article  PubMed  CAS  Google Scholar 

  35. Friedmann R, Toth M, Pena D, Mobashery S (1995) Activation of progelatinase B (MMP-9) by gelatinase A (MMP-2). Cancer Res 55:2548–2555

    Google Scholar 

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Otolaryngology, Kaunas University of Medicine, Eiveniu 2, 50009, Kaunas, Lithuania

    Virgilijus Uloza & Vykintas Liutkevičius

  2. Department of Cardiac Pathology, Institute of Cardiology, Kaunas University of Medicine, Kaunas, Lithuania

    Dalia Pangonytė

  3. Department of Physics, Mathematics and Biophysics, Kaunas University of Medicine, Kaunas, Lithuania

    Viktoras Šaferis

  4. Department of Molecular Cardiology, Institute of Cardiology, Kaunas University of Medicine, Kaunas, Lithuania

    Vaiva Lesauskaitė

Authors
  1. Virgilijus Uloza
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  2. Vykintas Liutkevičius
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  3. Dalia Pangonytė
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  4. Viktoras Šaferis
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  5. Vaiva Lesauskaitė
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Correspondence to Virgilijus Uloza.

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Uloza, V., Liutkevičius, V., Pangonytė, D. et al. Expression of matrix metalloproteinases (MMP-2 and MMP-9) in recurrent respiratory papillomas and laryngeal carcinoma: clinical and morphological parallels. Eur Arch Otorhinolaryngol 268, 871–878 (2011). https://doi.org/10.1007/s00405-011-1494-1

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  • DOI: https://doi.org/10.1007/s00405-011-1494-1

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