Skip to main content
SpringerLink
Log in

Nitrogen capture by grapevine roots and arbuscular mycorrhizal fungi from legume cover-crop residues under low rates of mineral fertilization

  • Original Paper
  • Published:
Biology and Fertility of Soils Aims and scope Submit manuscript

Abstract

The influence of mineral fertilization on root uptake and arbuscular mycorrhizal fungi-mediated 15N capture from labeled legume (Medicago polymorpha) residue was examined in winegrapes (Vitis vinifera) in the greenhouse, to evaluate compatibility of fertilization with incorporation of cover-crop residue in winegrape production. Plants grown in marginal vineyard soil were either fertilized with 0.25× Hoagland’s solution or not. This low fertilization rate represents the deficit management approach typical of winegrape production. Access to residue in a separate compartment was controlled to allow mycorrhizal roots (roots + hyphae), hyphae (hyphae-intact), or neither (hyphae-rotated) to proliferate in the residue by means of mesh core treatments. Leaves were weekly analyzed for 15N. On day 42, plants were analyzed for 15N and biomass; roots were examined for intraradical colonization; and soils were analyzed for 15N, inorganic N, Olsen-P, X-K, and extraradical colonization. As expected, extraradical colonization of soil outside the cores was unaffected by mesh core treatment, while that inside the cores varied significantly. 15N atom% excess was highest in leaves of roots + hyphae. In comparison, leaf 15N atom% excess in hyphae-intact was consistently intermediate between roots + hyphae and hyphae-rotated, the latter of which remained unchanged over time. Fertilization stimulated host and fungal growth, based on higher biomass and intraradical colonization of fertilized plants. Fertilization did not affect hyphal or root proliferation in residue but did lower %N derived from residue in leaves and stems by 50%. Our results suggest that even low fertilization rates decrease grapevine N uptake from legume crop residue by both extraradical hyphae and roots.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Allen MF (1991) The Ecology of Mycorrhizae. Cambridge University Press, Cambridge, UK

    Google Scholar 

  • Ames RN, Reid CPP, Porter LK, Cambardella C (1983) Hyphal uptake and transport of nitrogen from two 15N-labelled sources of Glomus mosseae, a vesicular-arbuscular mycorrhizal fungus. New Phytol 95:381–396

    Article  Google Scholar 

  • Avio L, Pellegrino E, Bonari E, Giovannetti M (2006) Functional diversity of arbuscular mycorrhizal fungal isolates in relation to extraradical mycelial networks. New Phytol 172:347–357

    Article  PubMed  Google Scholar 

  • Biricolti S, Ferrini F, Rinaldelli E, Tamantini I, Vignozzi N (1997) VAM fungi and soil lime content influence rootstock growth and nutrient content. Amer J Enol Vitic 48:93–99

    Google Scholar 

  • Boddington CL, Bassett EE, Jakobsen I, Dodd JC (1999) Comparison of techniques for the extraction and quantification of extra-radical mycelium of arbuscular mycorrhizal fungi in soils. Soil Biol Biochem 31:479–482

    Article  CAS  Google Scholar 

  • Cavagnaro TR, Smith FA, Smith SE, Jakobsen I (2005) Functional diversity of arbuscular mycorrhizae: exploitation of soil patches with different phosphate enrichment differs among fungal species. Plant Cell Environ 28:642–650

    Article  CAS  Google Scholar 

  • Cheng X, Baumgartner K (2004a) Arbuscular mycorrhizal fungi-mediated nitrogen transfer from vineyard cover crops to grapevines. Biol Fertil Soils 40:406–412

    Article  CAS  Google Scholar 

  • Cheng X, Baumgartner K (2004b) Survey of arbuscular mycorrhizal fungal communities in Northern California vineyards and mycorrhizal colonization potential of grapevine nursery stock. HortScience 39:1702–1706

    Google Scholar 

  • Cheng X, Baumgartner K (2006) Effects of mycorrhizal roots and extraradical hyphae on 15N uptake from vineyard cover crop litter and the soil microbial community. Soil Biol Biochem 38:2665–2675

    Article  CAS  Google Scholar 

  • Deal DR, Boothroyd CW, Mai WF (1971) Replanting of vineyards and its relationship to vesicular-arbuscular mycorrhiza. Phytopathology 62:172–175

    Article  Google Scholar 

  • Egerton-Warburton LM, Allen EB (2000) Shifts in arbuscular mycorrhizal communities along an anthropogenic nitrogen deposition gradient. Ecol Appl 10:484–496

    Article  Google Scholar 

  • Eissenstat DM (1992) Costs and benefits of constructing roots of small diameter. J Plant Nutrition 15:763–782

    Article  Google Scholar 

  • Epstein E (1972) Mineral Nutrition of Plants: Principles and Perspective. John Wiley & Sons, New York, NY

    Google Scholar 

  • Giovannetti M, Mosse B (1980) An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytol 84:489–500

    Article  Google Scholar 

  • Gryndler M, Larsen J, Hrselova H, Rezacova V, Gryndlerova H, Kubat J (2006) Organic and mineral fertilization, respectively, increase and decrease the development of external mycelium of arbuscular mycorrhizal fungi in a long-term field experiment. Mycorrhiza 16:159–166

    Article  PubMed  CAS  Google Scholar 

  • Hawkins H-J, George E (1999) Effect of plant nitrogen status on the contribution of arbuscular mycorrhizal hyphae to plant nitrogen uptake. Physiol Plant 105:694–700

    Article  CAS  Google Scholar 

  • Hawkins H-J, Johansen A, George E (2000) Uptake and transport of organic and inorganic nitrogen by arbuscular mycorrhizal fungi. Plant Soil 226:275–285

    Article  CAS  Google Scholar 

  • Hodge A (2001) Arbuscular mycorrhizal fungi influence decomposition of, but not plant nutrient capture from, glycine patches in soil. New Phytol 151:725–734

    Article  CAS  Google Scholar 

  • Hodge A (2003) N capture by Plantago lanceolata and Brassica napus from organic matter: the influence of spatial dispersion, plant competition and an arbuscular mycorrhizal fungus. J Exp Botany 54:2331–2342

    Article  CAS  Google Scholar 

  • Hodge A, Campbell CD, Fitter AH (2001) An arbuscular mycorrhizal fungus accelerates decomposition and acquires nitrogen directly from organic material. Nature 413:297–299

    Article  PubMed  CAS  Google Scholar 

  • Ingels CA, Scow KM, Whisson DA, Drenovsky RE (2005) Effects of cover crops on grapevines, yield, juice composition, soil microbial ecology, and gopher activity. Amer J Enol Vitic 56:19–29

    Google Scholar 

  • Jakobsen I, Abbott LK, Robson AD (1992) External hyphae of vesicular-arbuscular mycorrhizal fungi associated with Trifolium subterraneum L. 1. Spread of hyphae and phosphorus inflow into roots. New Phytol 120:371–380

    Article  CAS  Google Scholar 

  • Jin H, Pfeffer PE, Douds DD, Piotrowski E, Lammers PJ, Shachar-Hill Y (2005) The uptake, metabolism, transport and transfer of nitrogen in an arbuscular mycorrhizal symbiosis. New Phytol 168:687–696

    Article  PubMed  CAS  Google Scholar 

  • Johansen A, Jakobsen I, Jensen ES (1992) Hyphal transport of 15N-labelled nitrogen by a vesicular-arbuscular mycorrhizal fungus and its affect on depletion on inorganic soil N. New Phytol 122:281–288

    Article  CAS  Google Scholar 

  • Johansen A, Jakobsen I, Jensen ES (1994) Hyphal N transport by a vesicular-arbuscular mycorrhizal fungus associated with cucumber grown at three nitrogen levels. Plant Soil 160:1–9

    Article  CAS  Google Scholar 

  • Johnson NC, Rowland DL, Corkidi L, Egerton-Warburton LM, Allen EB (2003) Nitrogen enrichment alters mycorrhizal allocation at five mesic to semiarid grasslands. Ecology 84:1895–1908

    Article  Google Scholar 

  • Jumpponen A, Trowbridge J, Mandyam K, Johnson L (2005) Nitrogen enrichment causes minimal changes in arbuscular mycorrhizal colonization but shifts community composition-evidence from rDNA data. Biol Fertil Soils 41:217–224

    Article  CAS  Google Scholar 

  • Koske RE, Gemma JN (1989) A modified procedure for staining roots to detect VA mycorrhizas. Mycol Res 92:486–505

    Google Scholar 

  • Linderman RG, Davis EA (2001) Comparative response of selected grapevine rootstocks and cultivars to inoculation with different mycorrhizal fungi. Amer J Enol Vitic 52:8–11

    CAS  Google Scholar 

  • Mäder P, Vierheileg H, Streitwolf-Engel R, Boller T, Frey B, Christie P, Wiemken A (2000) Transport of 15N from a soil compartment separated by a polytetrafluoroethylene membrane to plant roots via the hyphae of arbuscular mycorrhizal fungi. New Phytol 146:155–161

    Article  Google Scholar 

  • Matthews MA, Ishii R, Anderson MM, O’Mahony M (1990) Dependence of wine sensory attributes on vine water status. J Sci Food Agric 51:321–335

    Article  CAS  Google Scholar 

  • Menge JA, Raski DJ, Lider LA, Johnson ELV, Jones NO, Kissler JJ, Hemstreet CL (1983) Interactions between mycorrhizal fungi, soil fumigation, and growth of grapes in California. Amer J Enol Vitic 34:117–121

    Google Scholar 

  • Miller RM, Reinhardt DR, Jastrow JD (1995) External hyphal production of vesicular-arbuscular mycorrhizal fungi in pasture and tallgrass prairie communities. Oecologia 103:17–23

    Article  Google Scholar 

  • Mullins MG, Bouquet A, Williams LE (1992) Biology of the Grapevine. Cambridge University Press, Cambridge, UK

    Google Scholar 

  • Nappi P, Jodice R, Luzzati A, Corino L (1985) Grapevine root system and VA mycorrhizae in some soils of Piedmont (Italy). Plant Soil 85:205–210

    Article  Google Scholar 

  • Newman EI (1966) A method of estimating the total length of root in a sample. J Appl Ecol 3:139

    Article  Google Scholar 

  • Oehl F, Sieverding E, Ineichen K, Ris E-A, Boller T, Wiemken A (2005) Community structure of arbuscular mycorrhizal fungi at different soil depths in extensively and intensively managed agroecosystems. New Phytol 165:273–283

    Article  PubMed  Google Scholar 

  • Olsson PA, Burleigh SH, van Aarle IM (2005) The influence of external nitrogen on carbon allocation to Glomus intraradices in monoxenic arbuscular mycorrhizae. New Phytol 168:677–686

    Article  PubMed  CAS  Google Scholar 

  • Patrick AE, Smith R, Keck K, Berry AM (2004) Grapevine uptake of 15N-labeled nitrogen derived from a winter-annual leguminous cover-crop mix. Amer J Enol Vitic 55:187–190

    Google Scholar 

  • Patrick-King A, Berry AM (2005) Vineyard delta 15-N, nitrogen and water status in perennial clover and bunch grass cover crop systems of California’s central valley. Agric Ecosyst Environ 109:262–272

    Article  Google Scholar 

  • Perret P, Weissenbach P, Schwager H, Heller WE, Koblet W (1983) Adaptive nitrogen-management: a tool for the optimization of N-fertilization in vineyards. Vitic Enol Sci 48:124–126

    Google Scholar 

  • Possingham JV, Groot-Obbink J (1971) Endotrophic mycorrhiza and the nutrition of grape vines. Vitis 10:120–130

    Google Scholar 

  • Schreiner RP (2005) Spatial and temporal variation of roots, arbuscular mycorrhizal fungi, and plant and soil nutrients in a mature Pinot noir (Vitis vinifera L.) vineyard in Oregon, USA. Plant Soil 276:219–234

    Article  CAS  Google Scholar 

  • Schreiner RP, Bethlenfalvay GJ (2003) Crop residue and Collembola interact to determine the growth of mycorrhizal pea plants. Biol Fertil Soils 39:1–8

    Article  Google Scholar 

  • Schubert A, Cammarata S, Eynard I (1988) Growth and root colonization of grapevines inoculated with different mycorrhizal endophytes. Hortscience 23:302–303

    Google Scholar 

  • St John TV, Coleman DC, Reid CPP (1983) Association of vesicular-arbuscular mycorrhizal hyphae with soil organic particles. Ecology 64:957–959

    Article  Google Scholar 

  • Treseder KK (2004) A meta-analysis of mycorrhizal responses to nitrogen, phosphorus, and atmospheric CO2 in field studies. New Phytol 164:347–355

    Article  Google Scholar 

  • Treseder KK, Allen MF (2002) Direct nitrogen and phosphorus limitation of arbuscular mycorrhizal fungi: a model and field test. New Phytol 155:507–515

    Article  Google Scholar 

Download references

Acknowledgements

We thank Bruce Mackey [Pacific West Area Statistician, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Albany, CA, USA] for advice on experimental design and means comparisons. Paul Schreiner (USDA-ARS, Corvallis, OR, USA), Kerri Steenwerth (USDA-ARS, Davis, CA, USA), Louise Jackson (Department. of Plant Sciences, University of California, Davis, CA, USA), and Alison Bennett (Department of Evolution and Ecology, University of California, Davis, CA, USA) provided helpful comments on this manuscript. Research was supported by USDA-ARS.

Author information

Authors and Affiliations

  1. Department of Biological Science, Mount St. Mary’s College, Los Angeles, CA, 90049, USA

    Xiaomei Cheng

  2. Agricultural Research Service, USDA, One Shields Avenue, Davis, CA, 95616, USA

    Amy Euliss & Kendra Baumgartner

Authors
  1. Xiaomei Cheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Amy Euliss
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kendra Baumgartner
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kendra Baumgartner.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cheng, X., Euliss, A. & Baumgartner, K. Nitrogen capture by grapevine roots and arbuscular mycorrhizal fungi from legume cover-crop residues under low rates of mineral fertilization. Biol Fertil Soils 44, 965–973 (2008). https://doi.org/10.1007/s00374-008-0281-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00374-008-0281-7

Keywords

Search

Navigation