Abstract
To investigate the effects of individual plant species on microbial community properties in soils of differing fertility, a microcosm experiment was carried out using plant species representative of the dominant flora in semi-fertile temperate grasslands of northern England. Soil microbial biomass and activity were found to be significantly greater in the more fertile, agriculturally improved soil than in the less productive unimproved meadow soil. Differences in microbial community structure were also evident between the two soils, with fungal abundance being greater in the unimproved soil type. Individual plant species effects significantly differed between the two soils. Holcus lanatus and Anthoxanthum odoratum stimulated microbial biomass in the improved soil type, but negatively affected this measure in the unimproved soil. In both soil types, herb species generally had negative effects on microbial biomass. Patterns for microbial activity were less consistent, but as with microbial biomass, A. odoratum and H. lanatus promoted respiration, whereas the herbs negatively affected this measure. All plant species grown in the improved soil increased the abundance of fatty acids synthesised by bacteria (bacterial phospholipid fatty acid analysis) relative to bare soil, but they negatively impacted on this group of fatty acids in unimproved soil. Similarly, the abundance of the fungal fatty acid 18:2ω6 was increased by all plants in the more fertile improved soil only, albeit non-significantly. Our data indicate that effects of plant species on microbial properties differ markedly in soils of differing fertility, making general predictions about how individual plants impact on soil properties difficult to make.
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References
Allen SE (1989) Chemical analysis of ecological materials, 2nd edn. Blackwell, Oxford
Bardgett RD, McAlister E (1999) The measurement of soil fungal:bacterial biomass ratios as an indicator of ecosystem self regulation in temperate meadow grasslands. Biol Fertil Soils 29:282–290
Bardgett RD, Shine A (1999) Linkages between plant litter diversity, soil microbial biomass and ecosystem function in temperate grasslands. Soil Biol Biochem 31:317–321
Bardgett RD, Hobbs PJ, Frostegård A (1996) Changes in soil fungal:bacterial biomass ratios following reductions in the intensity of management of an upland grassland. Biol Fertil Soils 22:261–264
Bardgett RD, Mawdsley JL, Edwards S, Hobbs PJ, Rodwell JS, Davies WJ (1999) Plant species and nitrogen effects on soil biological properties of temperate upland grasslands. Funct Ecol 13:650–660
Bever JD (1994) Feedback between plants and their soil communities in an old field community. Ecology 75:1965–1977
Bever JD, Westover KM, Antonovics J (1997) Incorporating the soil community into plant population dynamics: the utility of the feedback approach. J Ecol 85:561–573
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917
Chen J, Stark JM (2000) Plant species effects and carbon and nitrogen cycling in a sagebrush-crested wheatgrass soil. Soil Biol Biochem 32:47–57
Donnison LM, Griffith GS, Hedger J, Hobbs PJ, Bardgett RD (2000) Management influences on soil microbial communities and their function in botanically diverse haymeadows of northern England and Wales. Soil Biol Biochem 32:253–263
Federle TW (1986) Microbial distribution in soil—new techniques. In: Megusar F, Gantar M (eds) Perspectives in microbial ecology. Slovene Society for Microbiology, Ljubljana, pp 493–498
Frostegård A, Bååth E (1996) The use of phospholipid fatty acid analysis to estimate bacterial and fungal biomass in soil. Biol Fertil Soils 29:59–65
Frostegård A, Bååth E, Tunlid A (1993) Shifts in the structure of soil microbial communities in limed forests as revealed by phospholipid fatty acid analysis. Soil Biol Biochem 25:723–730
Gransee A, Wittenmayer L (2000) Qualitative and quantitative analysis of water-soluble root exudates in relation to plant species and development. J Plant Nutr Soil Sci 163:381–385
Grayston SJ, Wang S, Campbell CD, Edwards AC (1998) Selective influence of plant species on microbial diversity in the rhizosphere. Soil Biol Biochem 30:369–378
Groffman PM, Eagan P, Sullivan WM, Lemunyon JL (1996) Grass species and soil type effects on microbial biomass and activity. Plant Soil 183:61–67
Klironomos JN (2002) Feedback with soil biota contributes to plant rarity and invasiveness in communities. Nature 417:67–70
Mackey JML, Neal AM (1993) Section 2.5. Harvesting, recording weight, area and length. In: Hendry GAF, Grime JP (eds) Methods in comparative plant ecology. Chapman and Hall, London
Marschner H (1997) Mineral nutrition of higher plants, 2nd edn. Academic Press, London
Marschner P, Yang C-H, Lieberei R, Crowley DE (2001) Soil and plant specific effects on bacterial community composition in the rhizosphere. Soil Biol Biochem 33:1437–1445
Rodwell JS (1992) Grassland and montane communities. British plant communities, vol 3. Cambridge University Press, Cambridge
Rovira AD (1965) Plant root exudates and their influence upon soil micro-organisms. In: Baker KF, Snyder WC (eds) Ecology of soil-borne pathogens—prelude to biological control. University of California, Berkely, Calif., pp 170–186
Sparling GP, West AW, Feltham CW, Reynolds J (1990) Estimation of soil microbial C by a fumigation–extraction method: use on soils of high organic matter content and reassessment of the k ec-factor. Soil Biol Biochem 22:301–307
Tunlid A, Hoitink HAJ, Low C, White DC (1989) Characterization of bacteria that suppress Rhizoctonia damping-off in bark compost media by analysis of fatty acid biomarkers. Appl Environ Microbiol 55:1368–1374
Vance ED, Brookes PC, Jenkinson DS (1987) An extraction method for measuring soil microbial biomass C. Soil Biol Biochem 19:703–707
Van der Krift TAJ, Kuikman PJ, Möller F, Berendse F (2001) Plant species and nutritional-mediated control over rhizodeposition and root decomposition. Plant Soil 228:191–200
Vruggink H (1976) Influence of agricultural crops on the actinomycetes flora in soil. Plant Soil 44:639–654
Wardle DA, Nicholson KS (1996) Synergistic effects of grassland plant species on soil microbial biomass and activity: implications for ecosystem-level effects of enriched plant diversity. Funct Ecol 10:410–416
Wardle DA, Barker GM, Bonner KI, Nicholson KS (1998) Can comparative approaches based on plant ecophysiological traits predict the nature of biotic interactions and individual plant species effects in ecosystems? J Ecol 86:405–420
Wardle DA, Bonner KI, Barker GM, Yeates GW, Nicholson KS, Bardgett RD, Watson RN, Ghani A (1999) Plant removals in perennial grassland: vegetation dynamics, decomposers, soil biodiversity, and ecosystem properties. Ecol Monogr 69:535–568
White DC, Davis WM, Nickels JS, King JC, Bobbie RJ (1979) Determination of the sedimentary microbial biomass by extractable lipid phosphate. Oecologia 40:51–62
Zeller V, Bardgett RD, Tappeiner U (2001) Site and management effects on soil microbial properties of subalpine meadows: a study of land abandonment along a north-south gradient in the European Alps. Soil Biol Biochem 33:639–649
Acknowledgements
The authors are very grateful to Juliet Frankland for allowing us to use her meadows for soil sampling. This project was supported by a BBSRC studentship to Louise Innes.
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Innes, L., Hobbs, P.J. & Bardgett, R.D. The impacts of individual plant species on rhizosphere microbial communities in soils of different fertility. Biol Fertil Soils 40, 7–13 (2004). https://doi.org/10.1007/s00374-004-0748-0
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DOI: https://doi.org/10.1007/s00374-004-0748-0