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Relationship Between Endodormancy and Cold Hardiness in Grapevine Buds

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Abstract

Endodormancy (ED) and cold hardiness (CH) are two strategies utilized by grapevine (Vitis vinifera L.) buds to survive unfavorable winter conditions. Each phenomenon is triggered by different environmental cues—ED by short-day (SD) photoperiod and cold hardiness (CH) by low temperatures. In grapevine buds, CH occurs mainly via the supercooling of intracellular water, a phenomenon associated with the low temperature exotherm (LTE). The seasonal dynamics of ED and CH were studied on grapevines buds by determining the BR50 (time required to reach 50 % of bud break under forced conditions) and the LTE, which measure the depth of ED and the level of CH, respectively. Overlapping BR50 and LTE curves revealed that CH began to develop in late April, when buds were fully endodormant and daily mean temperatures had started to drop below 14 °C, suggesting that ED is a prerequisite for the acquisition of full CH. Increase in starch content and thickening of the cell wall (CW) of meristematic cells which occurs in dormant buds could be involved in structural and metabolic changes that favor CH subsequent acquisition. Interestingly, the thickening of the CW and the synthesis of starch which are associated with ED were induced by a SD-photoperiod, while the hydrolysis of starch, the accumulation of soluble sugars, and the up-regulation of dehydrin genes, which are associated with CH, were induced by low temperatures. Overall, the results indicate that structural, metabolic, and transcriptional changes that occur during ED in grapevine buds are necessary for the further development of CH.

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References

  • Arora R, Rowland LJ, Panta GR (1997) Chill-responsive dehydrins in blueberry: are they associated with cold-hardiness or dormancy transitions? Physiol Plant 101:8–16

    Article  CAS  Google Scholar 

  • Ashworth EN, Stirm VE, Volenec J (1993) Seasonal variations in soluble sugars and starch within woody stems of Cornus sericea L. Tree Physiol 13:379–388

    Article  CAS  PubMed  Google Scholar 

  • Badulescu R, Ernst M (2006) Changes of temperature exotherms and soluble sugar in grapevine (Vitis vinifera L) buds during winter. J Appl Bot Food Qual Angew Bot 80:165–170

    CAS  Google Scholar 

  • Burke MJ, Gusta LV, Quamme HA, Weiser CJ, Li PH (1976) Freezing and injury in plants. Ann Rev Plant Physiol 27:507–528

    Article  Google Scholar 

  • Chang S, Puryear J, Cairney JA (1993) Simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Rep 11:113–116

    Article  CAS  Google Scholar 

  • Dennis FG (2003) Problems in standardizing methods for evaluating the chilling requirements for the breaking of dormancy in buds of woody plants. Hortic Sci 38:347–350

    Google Scholar 

  • Fennell A, Hoover E (1991) Photoperiod influences growth, bud dormancy and cold acclimation of Vitis labruscana and V. riparia. J Am Soc Hortic Sci 116:270–273

    Google Scholar 

  • Ferguson JC, Tarara JM, Mills LJ, Grove GG, Keller M (2011) Dynamic thermal time model of cold hardiness for dormant grapevine buds. Ann Bot 107:389–396

    Article  PubMed Central  PubMed  Google Scholar 

  • Ferguson JC, Moyer MM, Mills LJ, Hoogenboom G, Keller M (2014) Modeling dormant bud cold hardiness and bud-break in twenty-three Vitis genotype reveals variation by region of origin. Am J Enol Vitic 65:59–71

    Article  Google Scholar 

  • Grant TNL, Gargrave J, Dami IE (2013) Morphological physiological, and biochemical changes in Vitis genotype in responses to photoperiod regimes. Am J Enol Vitic 64:466–475

    Article  Google Scholar 

  • Gunnink BW (1989) Critical analysis of conductometric phase transition porosimetry: i Capillary freezing and melting. Part Sci Techol 7:229–239

    Article  Google Scholar 

  • Gusta LV, Wisniewski M (2013) Understanding plant cold hardiness: an opinion. Plant Physiol 147:4–14

    Article  CAS  Google Scholar 

  • Hamman RA, Dami IE, Walsh TM, Stushnoff C (1996) Seasonal carbohydrate changes and cold hardiness of chardonnay and riesling grapevines. Am J Enol Vitic 47:31–36

    CAS  Google Scholar 

  • Hansen J, Moller IB (1975) Percolation of starch and soluble carbohydrates from plant tissue for quantitative determination with anthrone. Anal Biochem 68:87–94

    Article  CAS  PubMed  Google Scholar 

  • Hughes S, Graether SP (2011) Cryoprotective mechanism of a small intrinsically disordered dehydrin proteins. Prot Sci 20:42–50

    Article  CAS  Google Scholar 

  • Hughes S, Schart V, Malcomson J, Hogarth KA, Martynowicz DM, Tralman-Baker E, Patel SN, Graether SP (2013) The importance of size and disorder in the cryoprotective effects of dehydrins. Plant Physiol 163:1376–1386

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Jian L, Li PH, Sun L, Chen TH (1997) Alterations in ultrastructure and subcellular localization of Ca2+ in poplar apical bud cells during the induction of dormancy. J Exp Bot 48:1195–1207

    Article  CAS  Google Scholar 

  • Kosova K, Vitamvas P, Prasil IT (2007) The role of dehydrins in plant response to cold. Biol Plant 51:601–617

    Article  CAS  Google Scholar 

  • Koussa T, Broquedis M, Bouard J (1994) Changes of abscisic acid level during the development of grape latent buds, particularly in the phase of dormancy break. Vitis 33:63–67

    CAS  Google Scholar 

  • Kühn N, Ormeño J, Jaque-Zamora G, Pérez FJ (2009) Photoperiod modifies the diurnal expression profile of VvPHYA and VvPHYB transcript in field-grown grapevines. J Plant Physiol 166:1172–1180

    Article  PubMed  Google Scholar 

  • Lang GA (1987) Dormancy: a new universal terminology. Hortic Sci 22:817–820

    Google Scholar 

  • Levine H, Slade L (1980) Thermomechanical properties of small carbohydrates-water glasses and rubbers. J Chem Soc Faraday Trans 184:2619–2633

    Google Scholar 

  • Levitt J (1980) Freezing and high temperature stresses. Response of plants to environmental stresses, vol I. Academic Press, New York, pp 497–512

    Google Scholar 

  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real time quantitative PCR and the ΔΔCT method. Methods 25:402–408

    Article  CAS  PubMed  Google Scholar 

  • Mills LJ, Ferguson JC, Keller M (2006) Cold-hardiness evaluation of grapevine buds and cane tissues. Am J Enol Vitic 57:194–200

    Google Scholar 

  • Noriega X, Burgos B, Pérez FJ (2007) Short-day photoperiod triggers and low temperature increase expression of peroxidase RNA transcripts and basic peroxidase isoenzyme activity in grape-buds. Phytochemitry 68:1376–1383

    Article  CAS  Google Scholar 

  • Nylander M, Svensson J, Palva ET, Welin BV (2001) Stress-induced accumulation and tissue-specific localization of dehydrins in Arabidopsis thaliana. Plant Mol Biol 45:263–279

    Article  CAS  PubMed  Google Scholar 

  • Olsen JE, Junttila O, Nilsen J, Eriksson M, Martinussen I, Olsson O, Sandberg G, Moritz T (1997) Ectopic expression of phytochrome A in hybrid aspen changes critical day-length for growth and prevents cold acclimatization. Plant J 12:1339–1350

    Article  CAS  Google Scholar 

  • Pérez FJ, Rubio S, Ormeño-Nuñez J (2007) Is erratic bud-break in grapevines grown in warm winter areas related to disturbance in mitochondrial respiratory capacity and oxidative metabolism? Funct Plant Biol 34:624–632

    Article  Google Scholar 

  • Pierquet P, Stushnoff C (1980) Relationship of low temperature exotherms to cold injury in Vitis riparia Michx. Am J Enol Vitic 31:1–6

    Google Scholar 

  • Pierquet P, Stushnoff C, Low Burke MJ (1977) Temperature exotherms in stem and bud tissues of Vitis riparia Michx. J Am Chem Soc Hortic Sci 102:54–55

    Google Scholar 

  • Rinne PLH, Kaikuranta P, Van der Schoot C (2001) The shoot apical meristem restores its symplastic organization during chilling-induced release from dormancy. Plant J 26:249–264

    Article  CAS  PubMed  Google Scholar 

  • Rohde A, Bhalerao RP (2007) Plant dormancy in the perennial context. Trends Plant Sci 12:217–223

    Article  CAS  PubMed  Google Scholar 

  • Rozen S, Skaletsky H (2000) Primer3 on the www for general users and for biologist programmers. Methods Mol Biol 132:365–386

    CAS  PubMed  Google Scholar 

  • Rubio S, Donoso A, Pérez FJ (2014) The dormancy-breaking stimuli “chilling, hypoxia and cyanamide exposure” up-regulate the expression of α-amylase genes in grapevine buds. J Plant Physiol 171:373–381

    Article  CAS  PubMed  Google Scholar 

  • Sakai A, Larcher W (1987) Frost survival of plants: Responses and adaptations to freezing stress. Ecological studies, vol 62. Springer, Berlin

    Google Scholar 

  • Salzman RA, Bressan RA, Hasegawa PM, Ashworth EN, Bordelon BP (1996) Programmed accumulation of LEA-like protein during desiccation and cold acclimation of overwintering grape buds. Plant Cell Environ 19:713–720

    Article  CAS  Google Scholar 

  • Sreekantan L, Mathiason K, Grimplet J, Schlauch K, Dickerson JA, Fennell AY (2010) Differential floral development and gene expression in grapevines during long and short photoperiods suggests a role for floral genes in dormancy transitioning. Plant Mol Biol 73:191–205

    Article  CAS  PubMed  Google Scholar 

  • Thomashow MF (1999) Plant cold acclimation: freezing tolerance genes and regulatory mechanisms. Ann Rev Plant Physiol Plant Mol Biol 50:571–599

    Article  CAS  Google Scholar 

  • Tränker C, Lehmann S, Hoenicka H, Hanke M, Fladung M, Lenhardt D, Dunemann F, Gau A, Schlangen K, Malnoy M, Flachowsky H (2010) Over-expression of and FT homologous gene of apple induces early flowering in annual and perennial plants. Planta 232:1309–1324

    Article  Google Scholar 

  • Vergara R, Pérez FJ (2010) Similarities between natural and chemically induced bud-endodormancy release in grapevine Vitis vinifera L. Sci Hortic 125:648–653

    Article  CAS  Google Scholar 

  • Wake CMF, Fennell A (2000) Morphological, physiological and dormancy response of three Vitis genotypes to short photoperiod. Physiol Plant 109:203–210

    Article  CAS  Google Scholar 

  • Welling A, Rinne P, Viherä-Aamio Kontunen-Soppela S, Heino P, Palva ET (2004) Photoperiod and temperature differentially regulate the expression of two dehydrin genes during overwintering of birch (Betula pubescens Ehrh.). J Exp Bot 55:507–516

    Article  CAS  PubMed  Google Scholar 

  • Wolf TK, Cook MK (1994) Cold hardiness of dormant buds of grape cultivars: comparison of thermal analysis and field survival. Hortic Sci 29:453–455

    Google Scholar 

  • Xin H, Zhu W, Wang L, Xiang Y, Fang L, Li J, Sun X, Wang N, Londo JP, Li S (2013) Genome wide transcriptional profile analysis of Vitis amurensis and V. vinifera in response to cold stress. PLoS One 8(3):e5870

    Article  Google Scholar 

  • Yang Y, He M, Zhu Z, Li S, Xu Y, Zhang C, Singer SD, Wang Y (2012) Identification of dehydrin gene family from grapevine species and analysis of their responsiveness to various forms of abiotic and biotic stress. BMC Plant Biol 12:140–148

    Article  PubMed Central  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The financial support of FONDECYT Project 1140318 is gratefully acknowledged.

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Authors and Affiliations

  1. Laboratorio de Bioquímica Vegetal, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile

    Sebastián Rubio & Francisco J. Pérez

  2. Centro de Ciencias e Tecnologias Agropecuarias, Universidade Estadual do Norte Fluminense, Av Alberto Lamego 2000, Campos dos Goytacazes, RJ, Brazil

    Débora Dantas & Ricardo Bressan-Smith

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  1. Sebastián Rubio
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  2. Débora Dantas
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  3. Ricardo Bressan-Smith
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  4. Francisco J. Pérez
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Correspondence to Francisco J. Pérez.

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Rubio, S., Dantas, D., Bressan-Smith, R. et al. Relationship Between Endodormancy and Cold Hardiness in Grapevine Buds. J Plant Growth Regul 35, 266–275 (2016). https://doi.org/10.1007/s00344-015-9531-8

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  • DOI: https://doi.org/10.1007/s00344-015-9531-8

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