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Effects of Plant Growth Regulators and Saccharide on In Vitro Plant and Tuberous Root Regeneration of Cassava (Manihot esculenta Crantz)

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Abstract

A faster system to get tuberous roots from in vitro cultured cassava plants may enhance the process of exploring the function and practical application of some root-specific expressed genes. The effects of cytokinin, auxin, sucrose, maltose, and glucose on development of shoots and tuberous roots and plantlet regeneration of in vitro cultured cassava were investigated in this study. The cytokinin N-benzyladenine (BA) (0–2.0 mg l−1) was effective on shoot regeneration. The auxin α-naphthalene acetic acid (NAA) (0–2.0 mg l−1) proved to be effective on root development. Plantlets with fibrous roots easily generated tuberous roots in vitro. The tuberous roots were induced only when both BA and NAA were used in combination. MS medium supplemented with sucrose at 175 mM (or 6% w/v) resulted in the highest frequencies of shoot induction (71.43%) and average fresh weight (0.61 g) of tuberous roots when BA (0.5 mg l−1) and NAA (0.5 mg l−1) were also added to the MS medium.

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Acknowledgments

This study was supported by FAO/IAEA joint research program CPR-13189 and grants (2005) 1-7 and (2006) 4-3 from the Agriculture Committee of Shanghai Municipal Administration, the National Basic Research Program (grant 2007CB815701). The authors thank Prof. Roberto Tuberosa and Ms. Kay Tuberosa for comments and revisions on an early version of the manuscript.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, College of Plant Science, Huazhong Agricultural University, Wuhan, 430070, China

    Mingxia Fan, Liguo Zhou & Yunhua Liu

  2. Shanghai Agrobiological Gene Center, Shanghai, 201106, China

    Mingxia Fan, Zaochang Liu, Liguo Zhou, Tian Lin, Yunhua Liu & Lijun Luo

Authors
  1. Mingxia Fan
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  2. Zaochang Liu
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  3. Liguo Zhou
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  4. Tian Lin
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  5. Yunhua Liu
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  6. Lijun Luo
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Corresponding authors

Correspondence to Zaochang Liu or Lijun Luo.

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M. Fan and Z. Liu contributed equally to this work.

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Fan, M., Liu, Z., Zhou, L. et al. Effects of Plant Growth Regulators and Saccharide on In Vitro Plant and Tuberous Root Regeneration of Cassava (Manihot esculenta Crantz). J Plant Growth Regul 30, 11–19 (2011). https://doi.org/10.1007/s00344-010-9163-y

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  • DOI: https://doi.org/10.1007/s00344-010-9163-y

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