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Effect of explant type and genotype on the accumulation of bioactive compounds in adventitious root cultures of Polygonum multiflorum

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Abstract

The correlation between growth and accumulation of bioactive compounds was investigated in six genotypes of Polygonum multiflorum, grown in vitro, soil-grown (ex vitro), and as adventitious root cultures. Adventitious roots were induced on leaf and root explants from plantlets of the six genotypes grown in vitro. Line PM-06 achieved the highest biomass in vitro (0.91 g plant−1 FW; 0.1 g plant−1 (DW)), whereas line PM-05 produced the highest biomass ex vitro (54.67 g plant−1 FW; 12.93 g plant−1 DW). A comparison of the six lines found that total phenolic content (TPC) and total flavonoid content (TFC) were highest in roots from line PM-01 grown in vitro, but line PM-06 produced the highest levels of bioactive compounds in tubers (99.06 mg g−1 DW TPC; 42.31 mg g−1 DW TFC), which were 2.5-fold, 5.0-fold, and 4.8-fold higher than the highest levels produced by in vitro roots, in vitro shoots, and ex vitro shoots, respectively. Although adventitious root line AR-06 produced the greatest overall biomass (60.12 g L−1 FW; 6.36 g L−1 DW), bioactive content was highest in line AR-01 (50.35 mg g−1 DW TPC; 22.51 mg g−1 TFC). There was a strong correlation between phenolic production in plant roots grown in vitro and adventitious root lines, and plant roots grown ex vitro and adventitious root lines. Adventitious root cultures of P. multiflorum line AR-06 showed great potential for producing phenolic compounds. Such cultures may therefore provide an alternative to naturally grown plants as a potential biomass source for the production of bioactive compounds.

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Acknowledgements

This work was supported by the Korean Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through the Advanced Production Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (Grant Number 315013-4).

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Authors and Affiliations

  1. Department of Horticultural Science, Division of Animal, Horticulture and Food Sciences, Chungbuk National University, Cheongju, 28644, Republic of Korea

    Thanh-Tam Ho, Cheol-Seung Jeong & So-Young Park

  2. WellGreen Co., Ltd., Chungbuk National University, Cheongju, 28644, Republic of Korea

    Cheol-Seung Jeong

  3. Department of Forest Genetic Resources, National Institute of Forest Science, 39 Onjeong-ro, Suwon, 16631, Republic of Korea

    Hyoshin Lee

Authors
  1. Thanh-Tam Ho
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  2. Cheol-Seung Jeong
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  3. Hyoshin Lee
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  4. So-Young Park
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Contributions

T-TH acquired the data and wrote the manuscript. C-SJ and HL participated in data interpretation and revising of the manuscript to include important intellectual content. S-YP made substantial contributions to data interpretation, revising of the manuscript, the conception, and design of this study.

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Correspondence to So-Young Park.

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Communicated by Silvia Moreno.

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Key Message

The present study found strong correlations in phenolic compound production between roots, grown either in vitro or ex vitro, and adventitious root lines. Adventitious roots are thus a potential alternative source to naturally grown P. multiflorum for producing biomass and bioactive compounds.

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Ho, TT., Jeong, CS., Lee, H. et al. Effect of explant type and genotype on the accumulation of bioactive compounds in adventitious root cultures of Polygonum multiflorum. Plant Cell Tiss Organ Cult 137, 115–124 (2019). https://doi.org/10.1007/s11240-018-01556-5

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  • DOI: https://doi.org/10.1007/s11240-018-01556-5

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