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Effect of Auxins, Cytokinin and Activated Charcoal on In Vitro Propagation of Plantains Barraganete and Curare (Musa AAB)

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Abstract

The response to the application of auxins, cytokinin and activated charcoal (CA) on in vitro propagation and ex vitro acclimatization of two plantain cultivars (Musa AAB) was evaluated. Murashige and Skoog (MS) medium was used for crop establishment and the shoot multiplication. For rooting, MS medium was used with different auxin levels combined with CA. For acclimatization, in vitro plants placed in germination trays containing sterile substrate were used. In the establishment phase, low contamination was shown in both cultivars. During the in vitro multiplication phase, the highest average number of shoots per explant was obtained using 3 mg L−1 BAP + 0.1 mg L−1 IAA in the Curare cultivar and 4 mg L−1 BAP + 0.1 mg L−1 IAA in the Barraganete cultivar. The best treatments for in vitro rooting of both cultivars were MS + 0.3 mg/L IBA + 1 g/L CA. The two cultivars showed optimal levels of survival during acclimatization. There were, however, differences between the two cultivars for plant height and leaf emergence. The use of auxins in the rooting phase was important because of the number of physiological functions they regulate, while the use of CA induced rooting in both cultivars.

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Acknowledgments

The authors thank the Universidad Técnica de Manabí for financing this project through its degree scholarship program.

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Authors and Affiliations

  1. Agronomy Department, Technical University of Manabí (UTM), 130105, Portoviejo, Manabí, Ecuador

    Liliana Corozo Quiñonez, Fátima Macías Ponce, Marbelin Del Valle Moreira, Ana Moreira Mieles, Freddy Zambrano Gavilanes, Francisco Arteaga Alcívar, George Cedeño-García, Jefferson Vélez-Olmedo & Ramón Eduardo Jaimez

  2. Department of Pythopathology, University of Brasilia, Brasilia, Brazil

    Jefferson Vélez-Olmedo

  3. Agronomy Department, National University of Colombia, 2717000, Palmira, Cali, Colombia

    Liliana Corozo Quiñonez & Francisco Arteaga Alcívar

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  1. Liliana Corozo Quiñonez
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  2. Fátima Macías Ponce
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  3. Marbelin Del Valle Moreira
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Correspondence to Liliana Corozo Quiñonez.

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Significance Statement The influence of effect of auxins, cytokinins and activated carbon on in vitro plantain (Curare and Barraganete cultivars) propagation was proven and optimized. The cv. ‘Barraganete’ had a late response for visible changes in the explants in the establishment phase, while the cv. ‘Curare’ presented precocity for these changes.

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Corozo Quiñonez, L., Macías Ponce, F., Del Valle Moreira, M. et al. Effect of Auxins, Cytokinin and Activated Charcoal on In Vitro Propagation of Plantains Barraganete and Curare (Musa AAB). Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 91, 431–440 (2021). https://doi.org/10.1007/s40011-020-01218-7

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