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Arabidopsis Brassinosteroid Mutants det2-1 and bin2-1 Display Altered Salt Tolerance

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Abstract

Exogenous brassinosteroid (BR) has been reported to improve plant resistance to abiotic stress, but little is known about the role of endogenous BR in plant stress responses. In this study we investigated the involvement of endogenous BR in salt stress response using BR mutants det2-1 and bin2-1 of Arabidopsis. Seed germination and seedling growth of det2-1 and bin2-1 were more sensitive to salt stress than that of Columbia wild type (WT). The transcript levels of salt- and ABA-induced genes COR78 and P5CS1 were less induced in det2-1 than in WT under 200 mM NaCl. In addition, the basal proline level and, to a lesser extent, the proline level induced by 200 mM NaCl or 50 μM ABA in both det2-1 and bin2-1 was enhanced, resulting in decreased proline accumulation. On the other hand, exogenous 24-epibrassinolide (EBR) could enhance proline accumulation, promote root elongation of WT, and partially rescue the growth of det2-1 under salt stress. These results suggested that endogenous BR is positively involved in the plant response to salt stress in Arabidopsis.

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Acknowledgments

The authors are grateful to Dr. Zhiyong Wang (Stanford University) and Dr. Jun Zhao (The Chinese Academy of Agriculture Sciences) for providing Arabidopsis mutant seeds. This work was supported by the Chinese National Key Basic Research Project (#2006CB100100) from the Ministry of Science and Technology of China and the Hundred Talent Program of the Chinese Academy of Science.

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Authors and Affiliations

  1. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, People’s Republic of China

    Haitao Zeng, Qi Tang & Xuejun Hua

  2. Graduate School of Chinese Academy of Sciences, 100049, Beijing, People’s Republic of China

    Haitao Zeng & Qi Tang

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  1. Haitao Zeng
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  2. Qi Tang
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  3. Xuejun Hua
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Correspondence to Xuejun Hua.

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Haitao Zeng and Qi Tang contributed equally to this work.

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Zeng, H., Tang, Q. & Hua, X. Arabidopsis Brassinosteroid Mutants det2-1 and bin2-1 Display Altered Salt Tolerance. J Plant Growth Regul 29, 44–52 (2010). https://doi.org/10.1007/s00344-009-9111-x

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  • DOI: https://doi.org/10.1007/s00344-009-9111-x

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