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14-3-3 proteins contribute to leaf and root development via brassinosteroid insensitive 1 in Arabidopsis thaliana

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Abstract

Background

Brassinosteroids (BR) are essential growth hormone in plants. Various components involved in signal transduction pathway have been identified as targets of 14-3-3 phospho-binding proteins. Previously, we showed that 14-3-3 proteins directly interact with the Brassinosteroid Insensitive 1 (BRI1), the BR receptor kinase, and are also subject to phosphorylation in a BR-dependent manner.

Objective

In this study, we aimed to examine a potential interplay between 14-3-3 proteins and BRI1 in plant growth.

Methods

Morphological phenotypes of a T-DNA insertion mutant line, 14-3-3ψφε, defective in three 14-3-3 isoforms, psi, phi and epsilon, were characterized and compared with bri1-5 and two transgenic lines for BRI1, BRI1-Flag and BRI1-Flag (14-3-3ψφε). We also generated complementation lines carrying each of the three 14-3-3 genes and determined their differences in rosette growth.

Results

No significant differences between the wild-type and 14-3-3ψφε seedlings were observed regardless of BR applications. However, BRI1-Flag (14-3-3ψφε) showed a significantly reduced cold tolerance and BR sensitivity in hypocotyl and root development when compared to BRI1-Flag. In addition, narrower leaf shape and smaller rosette size were observed in BRI1-Flag (14-3-3ψφε), while the mutant phenotypes were partially restored in the complementation lines, two of which with 14-3-3φ and 14-3-3ε showed the rosette growth comparable to BRI1-Flag.

Conclusion

Taken together, our results suggested that 14-3-3 proteins might positively regulate BRI1 activity and showed that 14-3-3 isoforms have different functional impacts in BR signaling.

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Acknowledgements

This work was supported in part by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (No. NRF-2017R1A2B4004620), and by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through the Golden Seed Project, Ministry of Agriculture, Food and Rural Affairs (MAFRA) (213006-05-3-SBC30).

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Authors and Affiliations

  1. Smart Farm Research Center, Korea Institute of Science and Technology, Gangneung, Gangwon-do, 25451, Republic of Korea

    Jae Hoon Lee

  2. Department of Biological Sciences, College of Biological Sciences and Biotechnology, Chungnam National University, Daejeon, 34134, Republic of Korea

    Geunhwa Kwak & Man-Ho Oh

  3. Department of Horticulture, College of Agriculture and Life Science, Chungnam National University, Daejeon, 34134, Republic of Korea

    Yong Pyo Lim

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  1. Jae Hoon Lee
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Correspondence to Man-Ho Oh.

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Lee, J.H., Kwak, G., Lim, Y.P. et al. 14-3-3 proteins contribute to leaf and root development via brassinosteroid insensitive 1 in Arabidopsis thaliana. Genes Genom 42, 347–354 (2020). https://doi.org/10.1007/s13258-019-00909-4

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