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Highly fluorescent semiconductor core–shell CdTe–CdS nanocrystals for monitoring living yeast cells activity

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Abstract

Fluorescent semiconductor nanocrystals in quantum confinement regime (quantum dots) present several well-known features which make them very useful tools for biological labeling purposes. Low photobleaching rates, high chemical stability and active surface allowing conjugation to living cells explain the success of this labeling procedure over the commonly used fluorescent dyes. In this paper we report the results obtained with highly fluorescent core–shell CdTe–CdS (diameter=3–7 nm) colloidal nanocrystals synthesized in aqueous medium and conjugated to glucose molecules. The conjugated nanocrystals were incubated with living yeast cells, in order to investigate their glucose up-take activity in real time, by confocal microscopy analysis.

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Authors and Affiliations

  1. Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil

    P.M.A. de Farias, M.A. Motta, A.G. Castro-Neto, A.A.S. Vieira, D.C.N. Silva & A. Fontes

  2. Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil

    B.S. Santos & A.G. Brasil Jr.

  3. Departamento de Química Fundamental, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil

    F.D. Menezes & R. Ferreira

  4. Departamento de Eletrônica Quântica, Universidade Estadual de Campinas, 13083-970, Campinas, SP, Brazil

    C.L. Cesar

Authors
  1. P.M.A. de Farias
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  2. B.S. Santos
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  3. F.D. Menezes
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  4. A.G. Brasil Jr.
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  5. R. Ferreira
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  6. M.A. Motta
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  7. A.G. Castro-Neto
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  8. A.A.S. Vieira
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  9. D.C.N. Silva
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  10. A. Fontes
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  11. C.L. Cesar
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Corresponding author

Correspondence to P.M.A. de Farias.

Additional information

PACS

87.83.+a; 61.46.Hk; 81.07.Ta

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de Farias, P., Santos, B., Menezes, F. et al. Highly fluorescent semiconductor core–shell CdTe–CdS nanocrystals for monitoring living yeast cells activity. Appl. Phys. A 89, 957–961 (2007). https://doi.org/10.1007/s00339-007-4267-3

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  • DOI: https://doi.org/10.1007/s00339-007-4267-3

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