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The effects of growth regulators and a scanning electron microscope study of somatic embryogenesis in Antartic hair grass (Deschampsia antarctica Desv.)

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Abstract

Deschampsia antartctica Desv. is a type of grass that is physiologically and biochemically adapted to the extreme environmental conditions of the Antarctic continent, which is of interest to many investigators. To explore the potential use of somatic embryogenesis as a biotechnological tool for the mass micropropagation of this grass, the effects of three dosages of 2,4-dichlorophenoxyacetic acid, dicamba, and picloram were evaluated. The developmental and morphological stages of somatic embryo formation were evaluated using scanning electron microscopy (SEM). Plant regeneration was evaluated under the effects of different dosages of 6-benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA), alone and combined. The results indicated that a Murashige and Skoog basal medium supplemented with 3 mg/l of dicamba was the best for inducing somatic embryogenesis, while the combination of 1 mg/l BAP and 0.1 mg/l of NAA was the most efficient for the regeneration and development of the plants. This work demonstrates, for the first time with the use of SEM, that it is possible to apply somatic embryogenesis for the regeneration of superficial and morphological structures of somatic embryos in the species D. antarctica.

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Acknowledgments

We would like to thank Nicolas Nazal from Gene X-Press Chile for his help in revising this manuscript and to the Instituto Antartico Chileno (INACH) for allowing us to participate in the Expedición Científica Antártica (ECA-47) during January 2010. This work was funded by Uxmal S.A. and the Comisión Nacional de Investigación Científica y Tecnológica (CONICYT) Doctorate Fellowship No. 24091116 awarded to Jennifer Osorio.

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Authors and Affiliations

  1. Laboratorio de Fisiología y Biología Molecular Vegetal, Facultad de Ciencias Agropecuarias y Forestales, Universidad de la Frontera, Casilla 54-D, Temuco, Chile

    Jennifer Osorio & Ana Gutiérrez-Moraga

  2. Facultad de Ingeniería y Ciencias, Universidad Adolfo Ibáñez, Diagonal Las Torres 2640, Santiago, Chile

    Jennifer Osorio

  3. Facultad de Medicina, Universidad de Chile, Av. Independencia 1027, Santiago, Chile

    Claudia Calderón

  4. Vicerrectoría de Investigación y Postgrado, Universidad de La Frontera, Casilla 54-D, Temuco, Chile

    Manuel Gidekel

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  1. Jennifer Osorio
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  2. Claudia Calderón
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  3. Ana Gutiérrez-Moraga
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  4. Manuel Gidekel
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Correspondence to Manuel Gidekel.

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Osorio, J., Calderón, C., Gutiérrez-Moraga, A. et al. The effects of growth regulators and a scanning electron microscope study of somatic embryogenesis in Antartic hair grass (Deschampsia antarctica Desv.). Polar Biol 37, 217–225 (2014). https://doi.org/10.1007/s00300-013-1425-2

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  • DOI: https://doi.org/10.1007/s00300-013-1425-2

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