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Cold-inducible MaC2H2s are associated with cold stress response of banana fruit via regulating MaICE1

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Abstract

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MaC2H2s are involved in cold stress response of banana fruit via repressing the transcription of MaICE1.

Abstract

Although C2H2 zinc finger proteins have been found to be involved in banana fruit ripening through transcriptional controlling of ethylene biosynthetic genes, their involvement in cold stress of banana remains elusive. In this study, another C2H2-ZFP gene from banana fruit was identified, which was named as MaC2H2-3. Gene expression analysis revealed that MaC2H2-1, MaC2H2-2 and MaC2H2-3 were cold inducible in the peel of banana during low temperature storage. MaC2H2-3 functions as a transcriptional repressor and localizes predominantly in nucleus. Particularly, promoters of MaC2H2-2 and MaC2H2-3 were noticeably activated by cold as well, further indicating the potential roles of C2H2 in cold stress of banana. Moreover, MaC2H2-2 and MaC2H2-3 significantly repressed the transcription of MaICE1, a key component in cold signaling pathway. Overall, these findings suggest that MaC2H2s may take part in controlling cold stress of banana through suppressing the transcription of MaICE1, providing new insight of the regulatory basis of C2H2 in cold stress.

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Abbreviations

CBF:

C-repeat binding factor

COR:

Cold-regulated

DLR:

Dual-luciferase reporter

GFP:

Green fluorescence protein

ICE:

Inducer of CBF expression

ORF:

Open reading frame

RH:

Relative humidity

RT-qPCR:

Quantitative real-time PCR

TF:

Transcription factor

ZFP:

Zinc finger protein

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Acknowledgements

This study was supported in part by the National Key R&D Program of China (Grant no. 2018YFD0401304), High-level Pre-research Project of Zhejiang Shuren University (Grant No. KXJ0518107), Scientific Research Startup Project of Zhejiang Shuren University (Grant no. KXJ0517105) and Scientific Research Project of Zhejiang Provincial Education Department (Grant no. Y201840693).

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Authors and Affiliations

  1. Institute of Food Science, Zhejiang Academy of Agricultural Science, Key Laboratory of Post-Harvest Handing of Fruits, Ministry of Agriculture, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Key Laboratory of China Light Industry, Hangzhou, 310021, People’s Republic of China

    Yan-Chao Han

  2. College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou, 310015, People’s Republic of China

    Chang-Chun Fu

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  1. Yan-Chao Han
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Correspondence to Chang-Chun Fu.

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Communicated by Prakash Lakshmanan.

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Han, YC., Fu, CC. Cold-inducible MaC2H2s are associated with cold stress response of banana fruit via regulating MaICE1. Plant Cell Rep 38, 673–680 (2019). https://doi.org/10.1007/s00299-019-02399-w

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