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MpMYBS3 as a crucial transcription factor of cold signaling confers the cold tolerance of banana

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Abstract

Banana (Musa spp.) is an important tropical crops. Low temperature is one of the key environmental stresses, which greatly affects the global banana production. Different varieties of banana exhibit a high degree of genetic variability for cold tolerance. Compared with Cavendish banana, Dajiao has superior cold tolerance. Cloning of Dajiao cold-tolerant genes and characterization of their functions could reveal the molecular mechanism of cold tolerance in Dajiao. Our previous comparative transcriptome analysis of cold-sensitive Cavendish banana and cold-tolerant Dajiao identified several cold-tolerance candidate genes in Dajiao. In this study, a Dajiao candidate gene, MpMYBS3 (homolog of MYBS3 in rice), encoding a transcription factor, was cloned and characterized. Amino acid sequence alignments showed that MpMYBS3 belongs to the R1-type MYB transcription factors. Subcellular localization analysis indicated that MpMYBS3 is located in the nucleus. Heterologous overexpression of MpMYBS3 in banana showed that the transgenic lines had significantly higher cold tolerance than the wild-type, which might be associated with the increased accumulation of proline, and a reduction in malondialdehyde content and electrolyte leakage. Surprisingly, MYBS3 repressed the well-known ICE1–CBF-dependent cold signaling pathway in banana: the MaCBF1 and MaCBF2 genes were repressed at the transcriptional level after cold treatment. However, MaMKRY46 was significantly induced in transgenic bananas overexpressing MpMYBS3 under cold stress. These findings suggest that MYBS3-mediated cold signaling as a key player in cold adaptation of banana.

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Abbreviations

TF(s):

Transcription factor(s)

MW:

Molecular weight

PI:

Isoelectric point

CI:

Chilling injury

ECS(s):

Embryogenic cell suspension(s)

GUS:

β-Glucuronidase

WT:

Wild type

MDA:

Malondialdehyde

HTH:

Helix-turn-helix

NAA:

Naphthylacetic

Pro:

Proline

ILA:

Injured leaf area

TLA:

Total leaf area

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Acknowledgments

This work was supported by National Natural Science Foundation of China Nos. 31201589 and 31372018; Guangdong special support plan for youth talents of science and technology No. 2014TQ01N175; 948 Project from Ministry of Agriculture of China No. 2011-G16; National Banana Industry and Technology System Project No. CARS-32-01; International Collaborative Project from Ministry of Science and Technology of China No. 2013DFB30400; Commonwealth Industry (Agriculture) Specific Fund No. 200903049-10.

Author’s contribution

Tong-xin Dou, Xiao-xuan Sun, Xiu-hong Shao, Jun-hua Wu and Manosh-Kumar Biswas conceived of the study and analyzed the data. Tong-xin Dou, Chun-hua Hu, Li-jie Ding Jie Gao and Wei-di He performed the experiment. Tong-xin Dou and Qiao-song Yang drafted the manuscript. Qiao-song Yang and Gan-jun Yi revised the manuscript. All authors read and approved the final manuscript.

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Authors and Affiliations

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China

    Tong-xin Dou, Xiao-xuan Sun, Jun-hua Wu, Li-jie Ding, Jie Gao, Qiao-song Yang & Gan-jun Yi

  2. Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China

    Tong-xin Dou, Chun-hua Hu, Xiao-xuan Sun, Xiu-hong Shao, Jun-hua Wu, Li-jie Ding, Jie Gao, Wei-di He, Manosh-Kumar Biswas, Qiao-song Yang & Gan-jun Yi

  3. Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Beijing, China

    Tong-xin Dou, Chun-hua Hu, Xiao-xuan Sun, Xiu-hong Shao, Jun-hua Wu, Li-jie Ding, Jie Gao, Wei-di He, Manosh-Kumar Biswas, Qiao-song Yang & Gan-jun Yi

  4. Key Laboratory of Tropical and Subtropical Fruit Tree Research, No. 80 Dafeng 2nd Street, Tianhe District, Guangzhou, 510640, Guangdong Province, China

    Tong-xin Dou, Chun-hua Hu, Xiao-xuan Sun, Xiu-hong Shao, Jun-hua Wu, Li-jie Ding, Jie Gao, Wei-di He, Manosh-Kumar Biswas, Qiao-song Yang & Gan-jun Yi

  5. Horticulture and Landscape College, Hunan Agricultural University, Changsha, 410128, China

    Xiu-hong Shao

  6. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China

    Wei-di He

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  1. Tong-xin Dou
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  2. Chun-hua Hu
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Correspondence to Qiao-song Yang or Gan-jun Yi.

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Dou, Tx., Hu, Ch., Sun, Xx. et al. MpMYBS3 as a crucial transcription factor of cold signaling confers the cold tolerance of banana. Plant Cell Tiss Organ Cult 125, 93–106 (2016). https://doi.org/10.1007/s11240-015-0932-y

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