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Fine mapping of S37, a locus responsible for pollen and embryo sac sterility in hybrids between Oryza sativa L. and O. glaberrima Steud

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Abstract

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Hybrid sterility locus S37 between Oryza glaberrima and Oryza sativa results in both pollen and embryo sac sterility.

Abstract

Interspecific crossing between African cultivated rice Oryza glaberrima and Oryza sativa cultivars is hindered by hybrid sterility. To dissect the mechanism of interspecific hybrid sterility, we developed a near-isogenic line (NIL)-S37 using Dianjingyou1 (DJY1) as the recipient parent and an African cultivated rice variety as the donor parent. Empty pollen and embryo sac sterility were observed in F1 hybrids between DJY1 and NIL-S37. Cytological analyses showed that pollen abortion in the F1 hybrids occurred at the late binucleate stage due to a failure of starch accumulation in pollen grains. In addition, partial abortion of the embryo sac in the F1 hybrid was observed during function megaspore developing into mature embryo sac. Molecular analysis revealed that the semi-sterility was largely caused by the abortion of male and female gametophytes carrying the S37 allele from DJY1. A population of 25,600 plants derived from the hybrid DJY1/NIL-S37 was developed to fine map S37. Based on the physical location of molecular markers, S37 locus was finally delimited to a region of 205 kb on the short arm of chromosome 1 in terms of reference sequences of cv. Nipponbare. Interestingly, an about 97-kb DNA segment was deleted in the NIL-S37 based on BAC clone information of O. glaberrima. Fifty-four open reading frames (ORF) were predicted in this 205-kb region of DJY1, whereas only 31 ORFs were in that of NIL-S37. These results are valuable for cloning of S37 gene and further breaking reproductive isolation between Oryza glaberrima and Oryza sativa cultivars, as well as marker-assisted transferring of the corresponding neutral allele in rice breeding programs.

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Abbreviations

DAPI:

4′, 6-Diamidino-2-phenylindole

DJY1:

Dianjingyou1

InDel:

Insertion–deletion

MAS:

Marker-assisted selection

MMC:

Microspore mother cell

NIL:

Near-isogenic line

ORF:

Open reading frame

SSR:

Simple sequence repeat

TRD:

Transmission ratio distortion

WCLSM:

Whole-mount stain-clearing laser scanning confocal microscopy

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Acknowledgments

This research was supported by the grants from the Ministry of Agriculture Key Laboratory of the middle and lower reaches of the Yangtze River japonica rice biology and genetic breeding, National Transform Science and Technology Program (2013ZX08001004-002), the Chinese National High Technology Research and Development Program (“863” Program, Nos. 2014AA10A604).

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    Authors and Affiliations

    1. National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China

      Yumin Shen, Zhigang Zhao, Hongyang Ma, Xiaofeng Bian, Yang Yu, Xiaowen Yu, Haiyuan Chen, Linglong Liu, Wenwei Zhang, Ling Jiang & Jianmin Wan

    2. Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

      Jianmin Wan

    3. Food Crops Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650205, China

      Jiawu Zhou & Dayun Tao

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    1. Yumin Shen
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    Correspondence to Jianmin Wan.

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    Communicated by W. Harwood.

    Y. Shen and Z. Zhao contributed equally to this work.

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    Shen, Y., Zhao, Z., Ma, H. et al. Fine mapping of S37, a locus responsible for pollen and embryo sac sterility in hybrids between Oryza sativa L. and O. glaberrima Steud. Plant Cell Rep 34, 1885–1897 (2015). https://doi.org/10.1007/s00299-015-1835-4

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