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Fine mapping of a gene responsible for pollen semi-sterility in hybrids between Oryza sativa L. and O. glaberrima Steud

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Abstract

Asian cultivated rice (Oryza sativa L.) and African cultivated rice (Oryza glaberrima Steud.) are the two main cultivated rice species in the world, with strong heterosis in their F1 hybrids. However, hybrid sterility is a major barrier, although significant heterosis has been observed. In this study, an F1 pollen semi-sterility locus, S19, was identified on rice chromosome 3 by using near-isogenic lines derived from repeated backcross and marker-assisted selection. The typical pollen semi-sterility was observed in F1 hybrids between S19-NIL and Dianjingyou 1. Cytological study of pollen developmental stages indicated that pollen abortion occurred at the late binucleate stage because of a starch accumulation obstacle in some pollen grains. Molecular analysis revealed that the semi-sterility was caused by the abortion of most male gametophytes carrying the S19 allele from the japonica variety Dianjingyou 1. In a population of 12,780 F2 plants derived from S19-NIL/Dianjingyou 1, the S19 locus was fine-mapped to a chromosomal region of 54 kb based on BAC clones of cv. Nipponbare. Interestingly, an addition of a DNA fragment of about 89 kb to the 54-kb region was found in S19-NIL based on BAC clones of O. glaberrima. Gene prediction analysis identified 12 open reading frames (ORF) based on the region of Dianjingyou 1, while 32 ORFs were predicted in S19-NIL. Map-based cloning of this gene will help us to understand the underlying mechanism of hybrid sterility between the two cultivated rice species.

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Abbreviations

InDel:

Insertion–deletion

MAS:

Marker-assisted selection

MMC:

Microspore mother cell

NERICA:

New rice for Africa

NIL:

Near-isogenic line

ORF:

Open reading frame

QTL:

Quantitative trait locus

SUMO:

Small ubiquitin-like modifier

SSR:

Simple sequence repeat

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Acknowledgments

This research is supported by the Transgene Program of China (2009ZX08009-107B), 863 Program of China (2006AA10A101), National Natural Science Foundation of China (30800679), Program of New Teacher by Education Department (200803071003), Jiangsu Science and Technology Development Program (BG2006301), Jiangsu Province Agricultural Germplasm Gene Pool Program (sx(2008)g08) and the 111 project (B08025).

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Authors and Affiliations

  1. National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, 210095, Nanjing, China

    Yunhui Zhang, Zhigang Zhao, Jiawu Zhou, Ling Jiang, Xiaofeng Bian, Yong Wang, Chaolong Wang, Zhengzheng Zhong & Jianmin Wan

  2. Institute of Crop Science, Chinese Academy of Agricultural Sciences, 100081, Beijing, China

    Jiankang Wang & Jianmin Wan

  3. Food Crops Research Institute, Yunnan Academy of Agricultural Sciences, 650205, Kunming, China

    Jiawu Zhou & Dayun Tao

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  1. Yunhui Zhang
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  2. Zhigang Zhao
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  3. Jiawu Zhou
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  4. Ling Jiang
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  5. Xiaofeng Bian
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  6. Yong Wang
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  8. Zhengzheng Zhong
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  9. Jiankang Wang
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  10. Dayun Tao
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  11. Jianmin Wan
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Correspondence to Dayun Tao or Jianmin Wan.

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The authors Yunhui Zhang and Zhigang Zhao contributed equally to this work.

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Zhang, Y., Zhao, Z., Zhou, J. et al. Fine mapping of a gene responsible for pollen semi-sterility in hybrids between Oryza sativa L. and O. glaberrima Steud. Mol Breeding 28, 323–334 (2011). https://doi.org/10.1007/s11032-010-9485-2

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