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Alteration of osa-miR156e expression affects rice plant architecture and strigolactones (SLs) pathway

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Abstract

Key message

Overexpressing osa - miR156e in rice produced a bushy mutant and osa - miR156e regulation of tillering may do this through the strigolactones (SLs) pathway. Appropriate downregulation of osa - miR156 expression contributed to the improvement of plant architecture.

Abstract

Tillering is one of the main determinants for rice architecture and yield. In this study, a bushy mutant of rice was identified with increased tiller number, reduced plant height, prolonged heading date, low seed setting, and small panicle size due to a T-DNA insertion which essentially elevated the expression of osa-miR156e. Transgenic plants with constitutive expression of osa-miR156e also had the bushy phenotype, which showed osa-miR156 may control apical dominance and tiller outgrowth via regulating the strigolactones signaling pathway. Furthermore, the extent of impaired morphology was correlated with the expression level of osa-miR156e. In an attempt to genetically improve rice architecture, ectopic expression of osa-miR156e under the GAL4-UAS system or OsTB1 promoter was conducted. According to agronomic trait analysis, pTB1:osa-miR156e transgenic plants significantly improved the grain yield per plant compared to plants overexpressing osa-miR156e, even though the yield was still inferior to the wild type, making it a very interesting albeit negative result. Our results suggested that osa-miR156 could serve as a potential tool for modifying rice plant architecture through genetic manipulation of the osa-miR156 expression level.

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Acknowledgments

This research was supported by 863 Project Grant 2012AA10A303, National Natural Science Foundation of China Grant 30970172, and the Program for New Century Excellent Talents in University.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China

    Zhihui Chen, Xinqiang Gao & Jian Zhang

  2. Fujian Academy of Agricultural Sciences, Fuzhou, 350003, China

    Zhihui Chen

  3. China National Rice Research Institute, Hangzhou, 311400, China

    Jian Zhang

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  1. Zhihui Chen
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  2. Xinqiang Gao
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Correspondence to Zhihui Chen.

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Communicated by E. Guiderdoni.

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Chen, Z., Gao, X. & Zhang, J. Alteration of osa-miR156e expression affects rice plant architecture and strigolactones (SLs) pathway. Plant Cell Rep 34, 767–781 (2015). https://doi.org/10.1007/s00299-015-1740-x

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