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Teosinte Branched 1 modulates tillering in rice plants

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Abstract

Tillering is an important trait of cereal crops that optimizes plant architecture for maximum yield. Teosinte Branched 1 (TB1) is a negative regulator of lateral branching and an inducer of female inflorescence formation in Zea mays (maize). Recent studies indicate that TB1 homologs in Oryza sativa (rice), Sorghum bicolor and Arabidopsis thaliana act downstream of the auxin and MORE AUXILIARY GROWTH (MAX) pathways. However, the molecular mechanism by which rice produces tillers remains unknown. In this study, transgenic rice plants were produced that overexpress the maize TB1 (mTB1) or rice TB1 (OsTB1) genes and silence the OsTB1 gene through RNAi-mediated knockdown. Because lateral branching in rice is affected by the environmental conditions, the phenotypes of transgenic plants were observed in both the greenhouse and the paddy field. Compared to wild-type plants, the number of tillers and panicles was reduced and increased in overexpressed and RNAi-mediated knockdown OsTB1 rice plants, respectively, under both environmental conditions. However, the effect was small for plants grown in paddy fields. These results demonstrate that both mTB1 and OsTB1 moderately regulate the tiller development in rice.

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Acknowledgments

This work was supported by a Korea Research Foundation (KRF) grant funded by the Korean government (MEST) (No. F00003) and by a grant from the Next-Generation Biogreen 21 Program (Plant Molecular Breeding Center, No. PJ008125), Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Department of Plant Science, Research Institute for Agriculture and Life Sciences, and Plant Genomics and Breeding Institute, Seoul National University, Seoul, 151-921, Republic of Korea

    Min-Seon Choi, Eun-Byeol Koh, Joohyun Lee, Tae-Ho Ham, Hak Soo Seo & Hee-Jong Koh

  2. Soybean Genomics and Improvement Laboratory, USDA, ARS, Beltsville, MD, 20705, USA

    Mi-Ok Woo

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  1. Min-Seon Choi
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  2. Mi-Ok Woo
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  3. Eun-Byeol Koh
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Correspondence to Hee-Jong Koh.

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Communicated by D. Zaitlin.

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Choi, MS., Woo, MO., Koh, EB. et al. Teosinte Branched 1 modulates tillering in rice plants. Plant Cell Rep 31, 57–65 (2012). https://doi.org/10.1007/s00299-011-1139-2

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  • DOI: https://doi.org/10.1007/s00299-011-1139-2

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