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The grapevine transcription factor WRKY2 influences the lignin pathway and xylem development in tobacco

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Abstract

Previous work has shown that transgenic tobacco plants constitutively over-expressing the Vitis vinifera L. transcription factor VvWRKY2 exhibit reduced susceptibility to necrotrophic fungal pathogens, suggesting that this transcription factor plays a role in grapevine response to phytopathogens. The work presented here characterizes the modifications in cell wall structure observed in the stems and petioles of these transgenic plants. Histochemical stainings of stem and petiole cross-sections using phloroglucinol or Maüle reagents revealed a delay in xylem formation, particularly in the petioles, and differences in lignin composition. Evaluation of lignin quantity and quality showed a decrease in the syringyl/guaiacyl ratio in both stem and petioles. Expression analysis using RT-PCR and potato microarrays showed that tobacco plants over-expressing VvWRKY2 exhibited altered expression of genes involved in lignin biosynthesis pathway and cell wall formation. The ability of VvWRKY2 to activate the promoter of the VvC4H gene, which is involved in the lignin biosynthetic pathway, was confirmed by transient transcriptional activation assays in tobacco protoplasts. Moreover, in situ hybridization revealed that VvWRKY2 is specifically expressed in cells undergoing lignification in young grapevine stems. Together, these results confirm that VvWRKY2 plays a role in regulating lignification in grapevine, possibly in response to biotic or abiotic stresses.

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References

  • Achnine L, Blancaflor EB, Rasmussen S, Dixon RA (2004) Colocalization of l-phenylalanine ammonia-lyase and cinnamate 4-hydroxylase for metabolic channeling in phenylpropanoid biosynthesis. Plant Cell 16:3098–3109. doi:10.1105/tpc.104.024406

    Article  CAS  PubMed  Google Scholar 

  • Andersson-Gunnerås S, Mellerowicz EJ, Love J, Segerman B, Ohmiya Y, Coutinho PM, Nilsson P, Henrissat B, Moritz T, Sundberg B (2006) Biosynthesis of cellulose-enriched tension wood in Populus: global analysis of transcripts and metabolites identifies biochemical and developmental regulators in secondary wall biosynthesis. Plant J 45:144–165. doi:10.1111/j.1365-313X.2005.02584.x

    Article  PubMed  Google Scholar 

  • Anterola AM, Lewis NG (2002) Trends in lignin modification: a comprehensive analysis of the effects of genetic manipulations/mutations on lignification and vascular integrity. Phytochemistry 61:221–294. doi:10.1016/S0031-9422(02)00211-X

    Article  CAS  PubMed  Google Scholar 

  • Balestrieri C, Castaldo D, Giovane A, Quagliuolo L, Servillo L (1990) A glycoprotein inhibitor of pectin methylesterase in kiwi fruit (Actinidia chinensis). Eur J Biochem 193:183–187. doi:10.1111/j.1432-1033.1990.tb19321.x

    Article  CAS  PubMed  Google Scholar 

  • Barrière Y, Guillet C, Goffner D, Pichon M (2003) Genetic variation and breeding strategies for improved cell wall digestibility in annual forage crops. A review. Anim Res 52:193–228. doi:10.1051/animres:2003018

    Article  Google Scholar 

  • Barrière Y, Thomas J, Denoue D (2008) QTL mapping for lignin content, lignin monomeric composition, p-hydroxycinnamate content, and cell wall digestibility in the maize recombinant inbred line progeny F838 × F286. Plant Sci 175:585–595. doi:10.1016/j.plantsci.2008.06.009

    Article  Google Scholar 

  • Baucher M, Petit-Conil M, Boerjan W (2003) Lignin: genetic engineering and impact on pulping. Crit Rev Biochem Mol Biol 38:305–350. doi:10.1080/10409230391036757

    Article  CAS  PubMed  Google Scholar 

  • Bomal C, Bedon F, Caron S, Mansfield SD, Levasseur C, Cooke JE, Blais S, Tremblay L, Morency MJ, Pavy N, Grima-Pettenati J, Séguin A, Mackay J (2008) Involvement of Pinus taeda MYB1 and MYB8 in phenylpropanoid metabolism and secondary cell wall biogenesis: a comparative in planta analysis. J Exp Bot 59:3925–3939. doi:10.1093/jxb/ern23430

    Article  CAS  PubMed  Google Scholar 

  • Boudet AM (2007) Evolution and current status of research in phenolic compounds. Phytochemistry 68:2722–2735. doi:10.1016/j.phytochem.2007.06.012

    Article  CAS  PubMed  Google Scholar 

  • Boudet AM, Kajita S, Grima-Pettenati J, Goffner D (2003) Lignins and lignocellulosics: a better control of synthesis for new and improved uses. Trends Plant Sci 8:576–581. doi:10.1016/j.tplants.2003.10.001

    Article  CAS  PubMed  Google Scholar 

  • Choi CS, Sano H (2007) Abiotic-stress induces demethylation and transcriptional activation of a gene encoding a glycerophosphodiesterase-like protein in tobacco plants. Mol Genet Genomics 277:589–600. doi:10.1007/s00438-007-0209-1

    Article  CAS  PubMed  Google Scholar 

  • Croteau R, Karp F (1991) Origin of natural odorants. In: Muller P, Lamparsky D (eds) Perfumes: art, science and technology. Elsevier Applied Science, New York, pp 101–126

    Google Scholar 

  • Demura T, Fukuda H (2007) Transcriptional regulation in wood formation. Trends Plant Sci 12:64–70. doi:10.1016/j.tplants.2006.12.006

    Article  CAS  PubMed  Google Scholar 

  • Dence CW, Lin SY (1992) The determination of lignin. In: Lin SY, Dence CW (eds) Methods in lignin chemistry. Springer-Verlag, Berlin, pp 33–61

    Google Scholar 

  • Devaiah BN, Karthikeyan AS, Raghothama KG (2007) WRKY75 transcription factor is a modulator of phosphate acquisition and root development in Arabidopsis. Plant Physiol 143:1789–1801. doi:10.1104/pp.106.093971

    Article  CAS  PubMed  Google Scholar 

  • Donaldson LA (2001) Lignification and lignin topochemistry—an ultrastructural view. Phytochemistry 57:859–873. doi:10.1016/S0031-9422(01)00049-8

    Article  CAS  PubMed  Google Scholar 

  • Eulgem T, Somssich IE (2007) Networks of WRKY transcription factors in defense signaling. Curr Opin Plant Biol 10:366–371. doi:10.1016/j.pbi.2007.04.020

    Article  CAS  PubMed  Google Scholar 

  • Eulgem T, Rushton PJ, Robatzek S, Somssich IE (2000) The WRKY superfamily of plant transcription factors. Trends Plant Sci 5:199–206. doi:10.1016/S1360-1385(00)01600-9

    Article  CAS  PubMed  Google Scholar 

  • Farbos I, Mouras A, Bereterbide A, Glimelius K (2001) Defective cell proliferation in the floral meristem of alloplasmic plants of Nicotiana tabacum leads to abnormal floral organ development and male sterility. Plant J 26:131–142. doi:10.1046/j.1365-313x.2001.01011.x

    Article  CAS  PubMed  Google Scholar 

  • Fornale S, Sonbol FM, Maes T, Capellades M, Puigdomenech P, Rigau J, Caparros-Ruiz D (2006) Down-regulation of the maize and Arabidopsis thaliana caffeic acid O-methyltransferase genes by two new maize R2R3-MYB transcription factors. Plant Mol Biol 62:809–823. doi:10.1007/s11103-006-9058-2

    Article  CAS  PubMed  Google Scholar 

  • Franke R, McMichael CM, Meyer K, Shirley AM, Cusumano JC, Chapple C (2000) Modified lignin in tobacco and poplar plants over-expressing the Arabidopsis gene encoding ferulate 5-hydroxylase. Plant J 22:223–234. doi:10.1046/j.1365-313x.2000.00727.x

    Article  CAS  PubMed  Google Scholar 

  • Fukushima K (2001) Regulation of syringyl to guaiacyl ratio in lignin biosynthesis. J Plant Res 114:499–508. doi:10.1007/PL00014017

    Article  CAS  Google Scholar 

  • Goicoechea M, Lacombe E, Legay S, Mihaljevic S, Rech P, Jauneau A, Lapierre C, Pollet B, Verhaegen D, Chaubet-Gigot N, Grima-Pettenati J (2005) EgMYB2, a new transcriptional activator from Eucalyptus xylem, regulates secondary cell wall formation and lignin biosynthesis. Plant J 43:553–567. doi:10.1111/j.1365-313X.2005.02480.x

    Article  CAS  PubMed  Google Scholar 

  • González-Guzmán M, Apostolova N, Bellés JM, Barrero JM, Piqueras P, Ponce MR, Micol JL, Serrano R, Rodríguez PL (2002) The short-chain alcohol dehydrogenase ABA2 catalyzes the conversion of xanthoxin to abscisic aldehyde. Plant Cell 14:1833–1846. doi:10.1105/tpc.002477

    Article  PubMed  Google Scholar 

  • Guillaumie S, San-Clemente H, Deswarte C, Martinez Y, Lapierre C, Murigneux A, Barrière Y, Pichon M, Goffner D (2007) MAIZEWALL. Database and developmental gene expression profiling of cell wall biosynthesis and assembly in maize. Plant Physiol 143:339–363. doi:10.1104/pp.106.086405

    Article  CAS  PubMed  Google Scholar 

  • Hamberger B, Hahlbrock K (2004) The 4-coumarate:CoA ligase gene family in Arabidopsis thaliana comprises one rare, sinapate-activating and three commonly occurring isoenzymes. Proc Natl Acad Sci USA 101:2209–2214. doi:10.1073/pnas.0307307101

    Article  CAS  PubMed  Google Scholar 

  • Hawkins S, Boudet A (2003) Defence lignin and hydroxycinnamyl alcohol dehydrogenase activities in wounded Eucalyptus gunnii. For Path 33:339–352. doi:10.1046/j.1439-0329.2003.00308.x

    Article  Google Scholar 

  • Ishimaru K, Yano M, Aoki N, Ono K, Hirose T, Lin SY, Monna L, Sasaki T, Ohsugi R (2001) Toward the mapping of physiological and agronomic characters on a rice function map: QTL analysis and comparison between QTLs and expressed sequence tags. Theor Appl Genet 102:793–800. doi:10.1007/s001220000467

    Article  CAS  Google Scholar 

  • Jaillon O, Aury JM, Noel B, Policriti A, Clepet C, Casagrande A, Choisne N, Aubourg S, Vitulo N, Jubin C, Vezzi A, Legeai F, Hugueney P, Dasilva C, Horner D, Mica E, Jublot D, Poulain J, Bruyère C, Billault A, Segurens B, Gouyvenoux M, Ugarte E, Cattonaro F, Anthouard V, Vico V, Del Fabbro C, Alaux M, Di Gaspero G, Dumas V, Felice N, Paillard S, Juman I, Moroldo M, Scalabrin S, Canaguier A, Le Clainche I, Malacrida G, Durand E, Pesole G, Laucou V, Chatelet P, Merdinoglu D, Delledonne M, Pezzotti M, Lecharny A, Scarpelli C, Artiguenave F, Pè ME, Valle G, Morgante M, Caboche M, Adam-Blondon AF, Weissenbach J, Quétier F, Wincker P, French-Italian Public Consortium for Grapevine Genome Characterization (2007) The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla. Nature 449:463–467. doi:10.1038/nature06148

    Article  CAS  PubMed  Google Scholar 

  • Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901–3907

    CAS  PubMed  Google Scholar 

  • Jung H-J, Ni W, Chapple C, Meyer K (1999) Impact of lignin composition on cell-wall degradability in an Arabidopsis mutant. J Sci Food Agric 79:922–928

    Article  CAS  Google Scholar 

  • Kawaoka A, Ebinuma H (2001) Transcriptional control of lignin biosynthesis by tobacco LIM protein. Phytochemistry 57:1149–1157. doi:10.1016/S0031-9422(01)00054-1

    Article  CAS  PubMed  Google Scholar 

  • Kawaoka A, Kaothien P, Yoshida K, Endo S, Yamada K, Ebinuma H (2000) Functional analysis of tobacco LIM protein Ntlim1 involved in lignin biosynthesis. Plant J 22:289–301. doi:10.1046/j.1365-313x.2000.00737.x

    Article  CAS  PubMed  Google Scholar 

  • Ko JH, Han KH, Park S, Yang J (2004) Plant body weight-induced secondary growth in Arabidopsis and its transcription phenotype revealed by whole-transcriptome profiling. Plant Physiol 135:1069–1083. doi:10.1104/pp.104.038844

    Article  CAS  PubMed  Google Scholar 

  • Laempe D, Jahn M, Breese K, Schägger H, Fuchs G (2001) Anaerobic metabolism of 3-hydroxybenzoate by the denitrifying bacterium Thauera aromatica. J Bacteriol 183:968–979. doi:10.1128/JB.183.3.968-979.2001

    Article  CAS  PubMed  Google Scholar 

  • Lapierre C, Rolando C, Monties B (1986) Thioacidolysis of poplar lignin/identification of monomeric syringyl products and characterization of guaiacyl syringyl fractions. Holzforschung 40:113–118

    Article  CAS  Google Scholar 

  • Leplé JC, Dauwe R, Morreel K, Storme V, Lapierre C, Pollet B, Naumann A, Kang KY, Kim H, Ruel K, Lefèbvre A, Joseleau JP, Grima-Pettenati J, De Rycke R, Andersson-Gunnerås S, Erban A, Fehrle I, Petit-Conil M, Kopka J, Polle A, Messens E, Sundberg B, Mansfield SD, Ralph J, Pilate G, Boerjan W (2007) Down-regulation of cinnamoyl-coenzyme A reductase in poplar: multiple-level phenotyping reveals effects on cell wall polymer metabolism and structure. Plant Cell 19:3669–3691. doi:10.1105/tpc.107.054148

    Article  PubMed  Google Scholar 

  • Li L, Osakabe Y, Joshi CP, Chiang VL (1999) Secondary xylem-specific expression of caffeoyl-coenzyme A 3-O-methyltransferase plays an important role in the methylation pathway associated with lignin biosynthesis in loblolly pine. Plant Mol Biol 40:555–565. doi:10.1023/A:1006244325250

    Article  CAS  PubMed  Google Scholar 

  • Lian X, Xing Y, Yan H, Xu C, Li X, Zhang Q (2005) QTLs for low nitrogen tolerance at seedling stage identified using a recombinant inbred line population derived from an elite rice hybrid. Theor Appl Genet 112:85–96. doi:10.1007/s00122-005-0108-y

    Article  CAS  PubMed  Google Scholar 

  • Liu X, Bai X, Wang X, Chu C (2007) OsWRKY71, a rice transcription factor, is involved in rice defense response. J Plant Physiol 164:969–979. doi:10.1016/j.jplph.2006.07.006

    Article  CAS  PubMed  Google Scholar 

  • Luo M, Dennis ES, Berger F, Peacock WJ, Chaudhury A (2005) MINISEED3 (MINI3), a WRKY family gene, and HAIKU2 (IKU2), a leucine-rich repeat (LRR) KINASE gene, are regulators of seed size in Arabidopsis. Proc Natl Acad Sci USA 102:17531–17536. doi:10.1073/pnas.0508418102

    Article  CAS  PubMed  Google Scholar 

  • Marè C, Mazzucotelli E, Crosatti C, Francia E, Stanca AM, Cattivelli L (2004) Hv-WRKY38: a new transcription factor involved in cold- and drought-response in barley. Plant Mol Biol 55:399–416. doi:10.1007/s11103-004-0906-7

    Article  PubMed  Google Scholar 

  • Méchin V, Argillier O, Barrière Y, Mila I, Pollet B, Lapierre C (2000) Relationships of cell wall composition to in vitro cell-wall digestibility of maize inbred line stems. J Sci Food Agric 80:574–580. doi:10.1002/(SICI)1097-0010(200004)80:5

    Article  Google Scholar 

  • Meng H, Campbell WH (1998) Substrate profiles and expression of caffeoyl coenzyme A and caffeic acid O-methyltransferases in secondary xylem of aspen during seasonal development. Plant Mol Biol 38:513–520. doi:10.1023/A:1006071708728

    Article  CAS  PubMed  Google Scholar 

  • Miao Y, Laun T, Zimmermann P, Zentgraf U (2004) Targets of the WRKY53 transcription factor and its role during leaf senescence in Arabidopsis. Plant Mol Biol 55:853–867. doi:10.1007/s11103-004-2142-6

    CAS  PubMed  Google Scholar 

  • Mukhtar MS, Deslandes L, Auriac MC, Marco Y, Somssich IE (2008) The Arabidopsis transcription factor WRKY27 influences wilt disease symptom development caused by Ralstonia solanacearum. Plant J 56:935–947. doi:10.1111/j.1365-313X.2008.03651.x

    Article  CAS  PubMed  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497. doi:10.1111/j.1399-3054.1962.tb08052.x

    Article  CAS  Google Scholar 

  • Mzid R, Marchive C, Blancard D, Deluc L, Barrieu F, Corio-Costet MF, Drira N, Hamdi S, Lauvergeat V (2007) Over-expression of VvWRKY2 in tobacco enhances broad resistance to necrotrophic fungal pathogens. Physiol Plant 131:434–447. doi:10.1111/j.1399-3054.2007.00975.x

    Article  CAS  PubMed  Google Scholar 

  • Naoumkina M, He XZ, Dixon R (2008) Elicitor-induced transcription factors for metabolic reprogramming of secondary metabolism in Medicago truncatula. BMC Plant Biol 8:132–146. doi:10.1186/1471-2229-8-132

    Article  PubMed  Google Scholar 

  • Nishikubo N, Awano T, Banasiak A, Bourquin V, Ibatullin F, Funada R, Brumer H, Teeri TT, Hayashi T, Sundberg B, Mellerowicz EJ (2007) Xyloglucan endo-transglycosylase (XET) functions in gelatinous layers of tension wood fibers in poplar—a glimpse into the mechanism of the balancing act of trees. Plant Cell Physiol 48:843–855. doi:10.1093/pcp/pcm055

    Article  CAS  PubMed  Google Scholar 

  • Patzlaff A, Newman LJ, Dubos C, Whetten RW, Smith C, McInnis S, Bevan MW, Sederoff RR, Campbell MM (2003) Characterisation of PtMYB1, an R2R3-MYB from pine xylem. Plant Mol Biol 53:597–608. doi:10.1023/B:PLAN.0000019066.07933.d6

    Article  CAS  PubMed  Google Scholar 

  • Pavy N, Boyle B, Nelson C, Paule C, Giguère I, Caron S, Parsons LS, Dallaire N, Bedon F, Bérubé H, Cooke J, Mackay J (2008) Identification of conserved core xylem gene sets: conifer cDNA microarray development, transcript profiling and computational analyses. New Phytol 180:766–786. doi:10.1111/j.1469-8137.2008.02615.x

    Article  CAS  PubMed  Google Scholar 

  • Pinçon G, Maury S, Hoffmann L, Geoffroy P, Lapierre C, Pollet B, Legrand M (2001) Repression of O-methyltransferase genes in transgenic tobacco affects lignin synthesis and plant growth. Phytochemistry 57:1167–1176. doi:10.1016/S0031-9422(01)00098-X

    Article  PubMed  Google Scholar 

  • Rae AM, Tricker PJ, Bunn SM, Taylor G (2007) Adaptation of tree growth to elevated CO2: quantitative trait loci for biomass in Populus. New Phytol 175:59–69. doi:10.1111/j.1469-8137.2007.02091.x

    Article  CAS  PubMed  Google Scholar 

  • Rae AM, Pinel M, Bastien C, Sabatti M, Street N, Tucker J, Dixon C, Marron N, Dillen S, Taylor G (2008) QTL for yield in bioenergy Populus: identifying G × E interactions from growth at three contrasting sites. Tree Genet Genomes 4:97–112. doi:10.1007/s11295-007-0091-3

    Article  Google Scholar 

  • Raes J, Rohde A, Christensen JH, Van de Peer Y, Boerjan W (2003) Genome-wide characterization of the lignification toolbox in Arabidopsis. Plant Physiol 133:1051–1071. doi:10.1104/pp.103.026484

    Article  CAS  PubMed  Google Scholar 

  • Raiola A, Camardella L, Giovane A, Mattei B, De Lorenzo G, Cervone F, Bellincampi D (2004) Two Arabidopsis thaliana genes encode functional pectin methylesterase inhibitors. FEBS Lett 557:199–203. doi:10.1016/S0014-5793(03)01491-1

    Article  CAS  PubMed  Google Scholar 

  • Ramamoorthy R, Jiang SY, Kumar N, Venkatesh PN, Ramachandran S (2008) A comprehensive transcriptional profiling of the WRKY gene family in rice under various abiotic and phytohormone treatments. Plant Cell Physiol 49:865–879. doi:10.1093/pcp/pcn061

    Article  CAS  PubMed  Google Scholar 

  • Raventós D, Jensen AB, Rask MB, Casacuberta JM, Mundy J, San Segundo B (1995) A 20 bp cis-acting element is both necessary and sufficient to mediate elicitor response of a maize PRms gene. Plant J 7:147–155. doi:10.1046/j.1365-313X.1995.07010147.x

    Article  PubMed  Google Scholar 

  • Rensink WA, Lee Y, Liu J, Iobst S, Ouyang S, Buell CR (2005) Comparative analyses of six solanaceous transcriptomes reveal a high degree of sequence conservation and species specific transcripts. BMC Genomics 6:124–138. doi:10.1186/1471-2164-6-124

    Article  PubMed  Google Scholar 

  • Riboulet C, Guillaumie S, Méchin V, Bosio M, Pichon M, Goffner D, Lapierre C, Pollet B, Lefèvre B, Martinant JP, Barrière Y (2009) Kinetics of phenylpropanoid gene expression in maize growing internodes: relationships with cell wall deposition. Crop Sci 49:211–223. doi:10.2135/cropsci2008.03.0130

    Article  CAS  Google Scholar 

  • Robatzek S, Somssich IE (2001) A new member of the Arabidopsis WRKY transcription factor family, AtWRKY6, is associated with both senescence- and defence-related processes. Plant J 28:123–133. doi:10.1046/j.1365-313X.2001.01131.x

    Article  CAS  PubMed  Google Scholar 

  • Rohde A, Morreel K, Ralph J, Goeminne G, Hostyn V, De Rycke R, Kushnir S, Van Doorsselaere J, Joseleau JP, Vuylsteke M, Van Driessche G, Van Beeumen J, Messens E, Boerjan W (2004) Molecular phenotyping of the pal1 and pal2 mutants of Arabidopsis thaliana reveals far-reaching consequences on phenylpropanoid, amino acid, and carbohydrate metabolism. Plant Cell 16:2749–2771. doi:10.1105/tpc.104.023705

    Article  CAS  PubMed  Google Scholar 

  • Sarkanen K, Ludwig C (1971) Lignins: occurrence, formation, structure, and reactions. Wiley-Intersciences, New York

  • Scalliet G, Lionnet C, Le Bechec M, Dutron L, Magnard JL, Baudino S, Bergougnoux V, Jullien F, Chambrier P, Vergne P, Dumas C, Cock JM, Hugueney P (2006) Role of petal-specific orcinol O-methyltransferases in the evolution of rose scent. Plant Physiol 140:18–29. doi:10.1104/pp.105.070961

    Article  CAS  PubMed  Google Scholar 

  • Schmidt K, Heberle B, Kurrasch J, Nehls R, Stahl DJ (2004) Suppression of phenylalanine ammonia lyase expression in sugar beet by the fungal pathogen Cercospora beticola is mediated at the core promoter of the gene. Plant Mol Biol 55:835–852. doi:10.1007/s11103-004-2141-7

    CAS  PubMed  Google Scholar 

  • Shi C, Koch G, Ouzunova M, Wenzel G, Zein I, Lübberstedt T (2006) Comparison of maize brown-midrib isogenic lines by cellular UV-microspectrophotometry and comparative transcript profiling. Plant Mol Biol 62:697–714. doi:10.1007/s11103-006-9049-3

    Article  CAS  PubMed  Google Scholar 

  • Soler M, Serra O, Molinas M, Huguet G, Fluch S, Figueras M (2007) A genomic approach to suberin biosynthesis and cork differentiation. Plant Physiol 144:419–431. doi:10.1104/pp.106.094227

    Article  CAS  PubMed  Google Scholar 

  • Toquin V, Grausem B, Geoffroy P, Legrand M (2003) Structure of the tobacco caffeic acid O-methyltransferase (COMT) II gene: identification of promoter sequences involved in gene inducibility by various stimuli. Plant Mol Biol 52:495–509. doi:10.1023/A:1024810916909

    Article  CAS  PubMed  Google Scholar 

  • Tovar Torres J, Block A, Hahlbrock K, Somssich IE (1993) lnfluence of bacterial strain genotype on transient expression of plasmid DNA in plant protoplasts. Plant J 4:587–592. doi:10.1046/j.1365-313X.1993.04030587.x

    Article  Google Scholar 

  • Ülker B, Shahid Mukhtar M, Somssich IE (2007) The WRKY70 transcription factor of Arabidopsis influences both the plant senescence and defense signaling pathways. Planta 226:125–137. doi:10.1007/s00425-006-0474-y

    Article  PubMed  Google Scholar 

  • Vanholme R, Morreel K, Ralph J, Boerjan W (2008) Lignin engineering. Curr Opin Plant Biol 11:1–8. doi:10.1016/j.pbi.2008.03.005

    Article  Google Scholar 

  • Wang H, Hao J, Chen X, Hao Z, Wang X, Lou Y, Peng Y, Guo Z (2007) Over-expression of rice WRKY89 enhances ultraviolet B tolerance and disease resistance in rice plants. Plant Mol Biol 65:799–815. doi:10.1007/s11103-007-9244-x

    Article  CAS  PubMed  Google Scholar 

  • Weisshaar B, Armstrong GA, Block A, da Costa e Silva O, Hahlbrock K (1991) Light-inducible and constitutively expressed DNA-binding proteins recognizing a plant promoter element with functional relevance in light responsiveness. EMBO J 10:1777–1786

    CAS  PubMed  Google Scholar 

  • Wu KL, Guo ZJ, Wang HH, Li J (2005) The WRKY family of transcription factors in rice and Arabidopsis and their origins. DNA Res 12:9–26. doi:10.1093/dnares/12.1.9

    Article  CAS  PubMed  Google Scholar 

  • Xie Z, Zhang ZL, Zou X, Yang G, Komatsu S, Shen QJ (2006) Interactions of two abscisic-acid induced WRKY genes in repressing gibberellin signaling in aleurone cells. Plant J 46:231–242. doi:10.1111/j.1365-313X.2006.02694.x

    Article  CAS  PubMed  Google Scholar 

  • Xie Z, Zhang ZL, Hanzlik S, Cook E, Shen QJ (2007) Salicylic acid inhibits gibberellin-induced alpha-amylase expression and seed germination via a pathway involving an abscisic-acid-inducible WRKY gene. Plant Mol Biol 64:293–303. doi:10.1007/s11103-007-9152-0

    Article  CAS  PubMed  Google Scholar 

  • Yamamoto T, Taguchi-Shiobara F, Ukai Y, Sasaki T, Yano M (2001) Mapping quantitative trait loci for days-to-heading, and culm, panicle and internode lengths in a BC1F3 population using an elite rice variety, Koshihikari, as the recurrent parent. Breed Sci 51:63–71. doi:10.1270/jsbbs.51.63

    Article  CAS  Google Scholar 

  • Yang X, Tu L, Zhu L, Fu L, Min L, Zhang X (2008) Expression profile analysis of genes involved in cell wall regeneration during protoplast culture in cotton by suppression subtractive hybridization and macroarray. J Exp Bot 59:3661–3674. doi:10.1093/jxb/ern214

    Article  CAS  PubMed  Google Scholar 

  • Yokoyama R, Nishitani K (2001) A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis. Plant Cell Physiol 42:1025–1033. doi:10.1093/pcp/pce154

    Article  CAS  PubMed  Google Scholar 

  • Zhong R, Lee C, Zhou J, McCarthy RL, Ye ZH (2008) A battery of transcription factors involved in the regulation of secondary cell wall biosynthesis in Arabidopsis. Plant Cell 20:2763–2782. doi:10.1105/tpc.108.061325

    Article  CAS  PubMed  Google Scholar 

  • Zhou J, Lee C, Zhong R, Ye ZH (2009) MYB58 and MYB63 are transcriptional activators of the lignin biosynthetic pathway during secondary cell wall formation in Arabidopsis. Plant Cell 21:248–266. doi:10.1105/tpc.108.063321

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

We sincerely thank Johann Petit and Christophe Rothan (Functional genomic platform of Bordeaux) for technical support during microarray data processing. We are also grateful to Michel Hernould and members of IFR103 “Imagery and Cytology Technical Platform” (PTIC, Bordeaux) for their advice during microscopic observations. Finally, we thank Sarah Cookson for a critical review of the manuscript.

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  1. Rim Mzid

    Present address: Laboratoire de Physiologie Moléculaire des Plantes, Centre de Biotechnologie de Borj Cédria, BP 901, 2050, Hammam-Lif, Tunisie

Authors and Affiliations

  1. UMR 1287 Ecophysiologie et Génomique, Université de Bordeaux, INRA, Institut des Sciences de la Vigne et du Vin (ISVV), 210 Chemin de Leysotte, 33882, Villenave d’Ornon, France

    Sabine Guillaumie, Rim Mzid, Céline Léon, Imène Hichri, Agnès Destrac-Irvine, Claudine Trossat-Magnin, Serge Delrot & Virginie Lauvergeat

  2. UMR 206 Chimie Biologique, AgroParisTech, INRA, 78850, Thiverval-Grignon, France

    Valérie Méchin

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  1. Sabine Guillaumie
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  2. Rim Mzid
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Correspondence to Virginie Lauvergeat.

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Guillaumie, S., Mzid, R., Méchin, V. et al. The grapevine transcription factor WRKY2 influences the lignin pathway and xylem development in tobacco. Plant Mol Biol 72, 215–234 (2010). https://doi.org/10.1007/s11103-009-9563-1

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  • DOI: https://doi.org/10.1007/s11103-009-9563-1

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