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The constitutive expression of Chrysanthemum dichrum ICE1 in Chrysanthemum grandiflorum improves the level of low temperature, salinity and drought tolerance

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Abstract

The quality and productivity of chrysanthemum are severely compromised by various abiotic stresses. Here, we describe the isolation of CdICE1 from Chrysanthemum dichrum using RACE PCR, which shared identical nucleotide of ICE1 ORF from Chrysanthemum grandiflorum variety ‘Jinba’. CdICE1 contains a conserved bHLH domain, a nuclear localization domain, a S-rich motif and a ACT domain. The constitutive expression of CdICE1 in C. grandiflorum improved the tolerance of C. grandiflorum to low temperature/freezing, drought and salinity. When the transgene was inserted in the antisense direction, the expression of the endogenous ICE1 gene was down-regulated, and the level of the plant’s sensitivity to abiotic stress increased. The level of expression of CgDREBa and CgDREBb, activities of superoxide dismutase and peroxidase and the proline content were enhanced in the sense transgenic lines, and lowered in the antisense ones under stresses. In conclusion, CdICE1 represents a promising candidate for a biotechnological approach to improve the level of crop abiotic stress tolerance.

Key message Overexpression of CdICE1 in C. grandiflorum confers the stress tolerance via its regulation of CgDREB involved in the oxidative and osmotic homeostasis pathways.

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Acknowledgments

This work was supported by the Program for Hi-Tech Research, Jiangsu, China (Grant No. (BE2008307, BE2009317, BE2010303), the National Natural Science Foundation of China (Grant No. 30872064, 31071820, 31071825), the Fundamental Research Funds for the Central Universities (KYJ200907, KYZ201112), the Shanghai Key Science and Technology for Agriculture Promotion program ((2009) 3-3), and the Non-profit Industry Financial Program of the Ministry of Science and Technology of the People’s Republic of China (200903020).

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  1. College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

    Lin Chen, Yu Chen, Jiafu Jiang, Sumei Chen, Fadi Chen, Zhiyong Guan & Weimin Fang

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  1. Lin Chen
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  2. Yu Chen
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  3. Jiafu Jiang
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  5. Fadi Chen
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Correspondence to Fadi Chen.

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Communicated by K. Chong.

L. Chen and Y. Chen equally contributed to this work.

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Chen, L., Chen, Y., Jiang, J. et al. The constitutive expression of Chrysanthemum dichrum ICE1 in Chrysanthemum grandiflorum improves the level of low temperature, salinity and drought tolerance. Plant Cell Rep 31, 1747–1758 (2012). https://doi.org/10.1007/s00299-012-1288-y

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