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Effect of salt and drought stress on antioxidant enzymes activities and SOD isoenzymes of liquorice (Glycyrrhiza uralensis Fisch)

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Abstract

The effects of salinity and drought on the antioxidative system (SOD, POD, CAT) were studied in liquorice seedlings (Glycyrrhiza uralensis Fisch). The results showed that both salt and drought stresses could induce oxidative stress, as indicated by the increase level of lipid peroxidation. The activities of SOD and POD were up-regulated by salt and drought stress, while CAT activity decreased. An additional MnSOD isoenzyme was detected in liquorice subjected to 2%NaCl stress. The data also showed that although the activity of SOD was differentially influenced by drought and salinity, the changes of antioxidant enzyme activities subjected to drought stress follow a pattern similar to that subjected to salt stress, indicating that similar defensive systems might be involved in the oxidative stress injury in liquorice.

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Abbreviations

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

CAT:

Catalase

POD:

Peroxidase

MDA:

Malondialdehyde

RWC:

Relative water content

PVP:

Polyvinylpyrrolidon

EDTA:

Ethylenediaminetetraacetic acid

CK:

Control

TEMED:

N,N,N′,N′-Tetramethylethylenediamine

PEG:

Polyethylene glycol

PBS:

Phosphate buffer saline

NBT:

Nitroblue tetrazolium

dw:

Distilled water

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Acknowledgements

This research was supported by National High-Tech Research and Development Plan of China under grant No. 2002AA327070 and No.2004AA326060.

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Authors and Affiliations

  1. Key Laboratory of Ion Beam Bio-engineering, Institute of Plasma Physics, Chinese Academy of Sciences, Anhui, Hefei, China

    Yan Pan, Li Jun Wu & Zeng Liang Yu

  2. Institute of Radiation Medicine, Fudan University, Shanghai, China

    Yan Pan

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  1. Yan Pan
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  2. Li Jun Wu
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  3. Zeng Liang Yu
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Correspondence to Yan Pan.

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Pan, Y., Wu, L.J. & Yu, Z.L. Effect of salt and drought stress on antioxidant enzymes activities and SOD isoenzymes of liquorice (Glycyrrhiza uralensis Fisch). Plant Growth Regul 49, 157–165 (2006). https://doi.org/10.1007/s10725-006-9101-y

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  • DOI: https://doi.org/10.1007/s10725-006-9101-y

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