Abstract
On the basis of the results of gene chip analysis of the salt-tolerant wheat mutant RH8706-49 under conditions of salt stress, we identified and cloned an unknown salt-induced gene TaST (Triticum aestivum salt-tolerant). Real-time quantitative PCR analysis showed that the expression of the gene was induced by salt stress. Transgenic Arabidopsis plants overexpressing the TaST gene showed higher salt tolerance than the wild-type controls. Subcellular localization studies revealed that the protein encoded by this gene was in the nucleus. In comparison with wild-type controls, transgenic Arabidopsis plants accumulated more Ca2+, soluble sugar, and proline and less Na+ under salt stress. Real-time quantitative PCR analysis showed that Arabidopsis plants overexpressing TaST also showed increased expression of many stress-related genes. All these findings indicated that TaST can enhance the salt tolerance of transgenic Arabidopsis plants.
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Abbreviations
- TaST :
-
Triticum aestivum salt-tolerant
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This work was supported by the National Natural Science Fund [30971766]; the Hebei Provincial Natural Science Fund [No. C2009000278].
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Communicated by J. Zou.
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Huang, X., Wang, G., Shen, Y. et al. The wheat gene TaST can increase the salt tolerance of transgenic Arabidopsis . Plant Cell Rep 31, 339–347 (2012). https://doi.org/10.1007/s00299-011-1169-9
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DOI: https://doi.org/10.1007/s00299-011-1169-9