Abstract
The root microsomal proteomes of salt-tolerant and salt-sensitive wheat lines under salt stress were analyzed by two-dimensional electrophoresis and mass spectrum. A wheat V-H+-ATPase E subunit protein was obtained whose expression was enhanced by salt stress. In silicon cloning identified the full-length cDNA sequences of nine subunits and partial cDNA sequences of two subunits of wheat V-H+-ATPase. The expression profiles of these V-H+-ATPase subunits in roots and leaves of both salt-tolerant and salt-sensitive wheat lines under salt and abscisic acid (ABA) stress were analyzed. The results indicate that the coordinated enhancement of the expression of V-H+-ATPase subunits under salt and ABA stress is an important factor determining improved salt tolerance in wheat. The expression of these subunits was tissue-specific. Overexpression of the E subunit by transgenic Arabidopsis thaliana was able to enhance seed germination, root growth and adult seedling growth under salt stress.
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This project is sponsored by Hebei Provincial Natural Science Fund (No. 2007000250).
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Qian Zhao and Yan-Jun Zhao contributed equally to this work and are both to be considered first authors.
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Zhao, Q., Zhao, YJ., Zhao, BC. et al. Cloning and functional analysis of wheat V-H+-ATPase subunit genes. Plant Mol Biol 69, 33–46 (2009). https://doi.org/10.1007/s11103-008-9403-8
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DOI: https://doi.org/10.1007/s11103-008-9403-8