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Production of tetraploid plants of non apomictic citrus genotypes

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Abstract

Ploidy manipulation in Citrus is a major issue of current breeding programs aiming to develop triploid seedless mandarins to address consumer demands for seedless fruits. The most effective method to obtain triploid hybrids is to pollinate tetraploid non apomictic cultivars with pollen of diploid varieties. Such non apomictic tetraploid lines are not found in the citrus germplasm and need to be created. In this work we describe a new methodology based on in vitro shoot-tip grafting combined with treatment of the micro-grafted shoot-tip with colchicine and oryzalin to achieve chromosome doubling and a dechimerization procedure assisted by flow cytometry. Stable tetraploid plants of Clemenules, Fina and Marisol clementines and Moncada mandarin have been obtained directly from shoot tip grafting combined with colchicine and oryzalin treatments or after dechimerization of mixoploids plants (2x–4x). These stable tetraploid plants have been used in 4x × 2x hybridizations, to recover over 3,250 triploid hybrids in 3 years.

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Acknowledgments

This work is jointly financed by the AGL2008-00596-MCI and Prometeo 2008/121 Generalitat Valenciana projects.

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Authors and Affiliations

  1. Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113, Moncada, Valencia, Spain

    Pablo Aleza, José Juárez & Luis Navarro

  2. UPR amélioration génétique des espèces à multiplication végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), IVIA, Ctra. Moncada-Náquera km 4.5, 46113, Moncada, Valencia, Spain

    Patrick Ollitrault

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  1. Pablo Aleza
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  2. José Juárez
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  3. Patrick Ollitrault
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  4. Luis Navarro
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Correspondence to Luis Navarro.

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Communicated by W. Harwood.

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Aleza, P., Juárez, J., Ollitrault, P. et al. Production of tetraploid plants of non apomictic citrus genotypes. Plant Cell Rep 28, 1837–1846 (2009). https://doi.org/10.1007/s00299-009-0783-2

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  • DOI: https://doi.org/10.1007/s00299-009-0783-2

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