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Induced parthenogenesis in mandarin for haploid production: induction procedures and genetic analysis of plantlets

  • Cell Biology and Morphogenesis
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Abstract

This study focused on haploid induction in mandarin through in situ gynogenesis by pollination with irradiated pollen of ‘Meyer’ lemon. Pollination was carried out for three genotypes of mandarin with four levels of gamma-ray-irradiated pollen (150, 300, 600, and 900 Gy). The resulting seeds were characterised by a small size. Embryos were rescued in vitro and the ploidy level of the plantlets was determined by flow cytometry analysis. Haploid, diploid, triploid plantlets were obtained. The haploid parthenogenetic origin was confirmed using microsatellite marker analysis and chromosome count. Diploid and triploid plants were the result of crosses between mandarin and lemon. The induction of gynogenetic haploids of ‘Fortune’ (Citrus clementina Hort ex Tan. × Citrus tangerina Hort ex Tan.) and ‘Ellendale’ (Citrus reticulata Blanco × Citrus sinensis L. Osb) is reported here for the first time.

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Acknowledgments

This work was supported by the “Collectivité Territoriale Corse”.

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Authors and Affiliations

  1. Unité de Recherche Multiplication végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), San Giuliano, 20230, France

    Yann Froelicher, Jean-Baptiste Bassene & Raphael Morillon

  2. Laboratoire de physiologie et biotechnologie végétale, Institut National de Recherche Agronomique de Tunisie, Ariana, 2049, Tunisia

    Emna Jedidi-Neji

  3. Unité de Recherche Multiplication végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Montpellier, 34398, France

    Dominique Dambier & Patrick Ollitrault

  4. Unité de Génétique et écophysiologie de la qualité des agrumes, Institut National de Recherche Agronomique (INRA), San Giuliano, 20230, France

    Geneviève Bernardini & Gilles Costantino

Authors
  1. Yann Froelicher
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  2. Jean-Baptiste Bassene
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  3. Emna Jedidi-Neji
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  4. Dominique Dambier
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  5. Raphael Morillon
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  6. Geneviève Bernardini
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  7. Gilles Costantino
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  8. Patrick Ollitrault
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Corresponding author

Correspondence to Yann Froelicher.

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Communicated by L. Peña.

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Froelicher, Y., Bassene, JB., Jedidi-Neji, E. et al. Induced parthenogenesis in mandarin for haploid production: induction procedures and genetic analysis of plantlets. Plant Cell Rep 26, 937–944 (2007). https://doi.org/10.1007/s00299-007-0314-y

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  • DOI: https://doi.org/10.1007/s00299-007-0314-y

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