Skip to main content
SpringerLink
Log in

A synthetic auxin (NAA) suppresses secondary wall cellulose synthesis and enhances elongation in cultured cotton fiber

  • Cell Biology and Morphogenesis
  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Use of a synthetic auxin (naphthalene-1-acetic acid, NAA) to start (Gossypium hirsutum) ovule/fiber cultures hindered fiber secondary wall cellulose synthesis compared with natural auxin (indole-3-acetic acid, IAA). In contrast, NAA promoted fiber elongation and ovule weight gain, which resulted in larger ovule/fiber units. To reach these conclusions, fiber and ovule growth parameters were measured and cell wall characteristics were examined microscopically. The differences in fiber from NAA and IAA culture were underpinned by changes in the expression patterns of marker genes for three fiber developmental stages (elongation, the transition stage, and secondary wall deposition), and these gene expression patterns were also analyzed quantitatively in plant-grown fiber. The results demonstrate that secondary wall cellulose synthesis: (1) is under strong transcriptional control that is influenced by auxin; and (2) must be specifically characterized in the cotton ovule/fiber culture system given the many protocol variables employed in different laboratories.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • An C, Saha S, Jenkins JN et al (2007) Transcriptome profiling, sequence characterization, and SNP-based chromosomal assignment of the EXPANSIN genes in cotton. Mol Genet Genomics 278:539–553

    Article  PubMed  CAS  Google Scholar 

  • Beasley CA (1977) Temperature-dependent response to indoleacetic acid is altered by NH4+ in cultured cotton ovules. Plant Physiol 59:203–206

    Article  PubMed  CAS  Google Scholar 

  • Beasley CA, Ting IP (1973) The effects of plant growth substances on in vitro fiber development from fertilized cotton ovules. Am J Bot 60:130–139

    Article  CAS  Google Scholar 

  • Beyer EM Jr, Morgan PW (1970) Effect of ethylene on the uptake, distribution, and metabolism of indoleacetic acid-1–14C and -2–14C and naphthaleneacetic acid-1–14C. Plant Physiol 46:157–162

    Article  PubMed  CAS  Google Scholar 

  • Davidonis GH (1993) Cotton fiber in vitro. In: Basra AS (ed) Cotton fiber: developmental biology, quality improvement, and textile processing. Hayworth Press Inc, New York, pp 65–84

    Google Scholar 

  • Delbarre A, Muller P, Imhoff V et al (1996) Comparison of mechanisms controlling uptake and accumulation of 2, 4-dichlorophenoxy acetic acid, naphthalene-1-acetic acid and indole-3-acetic acid in suspension-cultured tobacco cells. Planta 198:532–541

    Article  CAS  Google Scholar 

  • Delmer DP, Pear JR, Andrawis A et al (1995) Genes encoding small GTP-binding proteins analogous to mammalian rac are preferentially expressed in developing cotton fibers. Mol Gen Genet 248:43–51

    Article  PubMed  CAS  Google Scholar 

  • Hackett DP, Thimann KV (1952) The nature of the auxin-induced water uptake by potato tissue. Am J Bot 39:553–560

    Article  CAS  Google Scholar 

  • Haigler CH, Rao NR, Roberts EM et al (1991) Cultured ovules as models for cotton fiber development under low temperatures. Plant Physiol 95:88–96

    Article  PubMed  CAS  Google Scholar 

  • Haigler CH, Zhang D, Wilkerson CG (2005) Biotechnological improvement of cotton fibre maturity. Physiol Plant 124:285–294

    Article  CAS  Google Scholar 

  • Haigler CH, Singh B, Wang G (2009) Genomics of cotton fiber secondary wall deposition and cellulose biogenesis. In: Paterson AH et al (eds) Genetics and genomics of cotton, plant genetics and genomics: crops and models 3. Springer Science + Business Media, LLC, New York, pp 385–417

    Google Scholar 

  • Harmer SE, Orford SJ, Timmis JN (2002) Characterisation of six a-expansin genes in Gossypium hirsutum (upland cotton). Mol Genet Genomics 268:1–9

    Article  PubMed  CAS  Google Scholar 

  • Jasdanwala RT, Singh YD, Chinoy JJ (1977) Auxin metabolism in developing cotton hairs. J Exp Bot 28:1111–1116

    Article  CAS  Google Scholar 

  • John ME (1999) Genetic engineering strategies for cotton fiber modification. In: Basra AS (ed) Cotton fiber: developmental biology, quality improvement, and textile processing. Hayworth Press Inc, New York, pp 271–292

    Google Scholar 

  • Joshi CP, Mansfield SD (2007) The cellulose paradox—simple molecule, complex biosynthesis. Curr Opin Plant Biol 10:220–226

    Article  PubMed  CAS  Google Scholar 

  • Laosinchai W, Cui X, Brown RM Jr (2000) A full length cDNA of cotton cellulose synthase has high homology with the Arabidopsis rsw1 gene and the cotton Cel1 gene (Accession No. AF200553) (PGR00-002). Plant Physiol 122:291

    Article  Google Scholar 

  • Martin LK, Haigler CH (2004) Cool temperature hinders flux from glucose to sucrose during cellulose synthesis in secondary wall stage cotton fibers. Cellulose 11:339–349

    Article  CAS  Google Scholar 

  • McQueen-Mason SJ, Cosgrove DJ (1995) Expansin mode of action on cell walls. Analysis of wall hydrolysis, stress relaxation, and binding. Plant Physiol 107:87–100

    PubMed  CAS  Google Scholar 

  • Meinert MC, Delmer DP (1977) Changes in biochemical composition of the cell wall of the cotton fiber during development. Plant Physiol 59:1088–1097

    Article  PubMed  CAS  Google Scholar 

  • Naithani SC, Rao NR, Singh YD (1982) Physiological and biochemical changes associated with cotton fiber development I. Growth kinetics and auxin content. Physiol Plant 54:225–229

    Article  CAS  Google Scholar 

  • Pear JR, Kawagoe Y, Schreckengost WE et al (1996) Higher plants contain homologs of the bacterial celA genes encoding the catalytic subunit of cellulose synthase. Proc Natl Acad Sci USA 93:12637–12642

    Article  PubMed  CAS  Google Scholar 

  • Peng L, Kawagoe Y, Hogan P et al (2002) Sitosterol-ß-glucoside as primer for cellulose synthesis in plants. Science 295:147–150

    Article  PubMed  CAS  Google Scholar 

  • Potikha TS, Collins CC, Johnson et al (1999) The involvement of hydrogen peroxide in the differentiation of secondary walls in cotton fibers. Plant Physiol 119:849–858

  • Powell RD (1969) Effect of temperature on boll set and development of Gossypium hirsutum. Cotton Grow Rev 46:29–36

    Google Scholar 

  • Roberts EM, Rao NR, Huang JY et al (1992) Effects of cycling temperatures on fiber metabolism in cultured cotton ovules. Plant Physiol 100:979–986

    Article  PubMed  CAS  Google Scholar 

  • Schubert AM, Benedict CR, Berlin JD et al (1973) Cotton fiber development -kinetics of cell elongation and secondary wall thickening. Crop Sci 13:704–709

    Article  Google Scholar 

  • Shi YH, Zhu SW, Mao XZ et al (2006) Transcriptome profiling, molecular, biological, and physiological studies reveal a major role for ethylene in cotton fiber cell elongation. Plant Cell 18:651–664

    Article  PubMed  CAS  Google Scholar 

  • Sun Y, Veerabomma S, Abdel-Mageed HA et al (2005) Brassinosteroid regulates fiber development on cultured cotton ovules. Plant Cell Physiol 46:1384–1391

    Article  PubMed  CAS  Google Scholar 

  • Tan X, Calderon-Villabolos LIA, Sharon M et al (2007) Mechanism of auxin perception by the TIR1 ubiquitin ligase. Nature 446:640–645

    Article  PubMed  CAS  Google Scholar 

  • Triplett BA (2000) Cotton ovule culture: a tool for basic biology, biotechnology, and cotton improvement. In Vitro Cell Dev Biol Plant 36:93–101

    Article  Google Scholar 

  • Triplett BA, Timpa JD (1995) Characterization of cell-wall polymers from cotton ovule culture fiber cells by gel permeation chromatography. In vitro Cell Dev Biol Plant 31:171–175

    CAS  Google Scholar 

  • Wäfler U, Meier H (1994) Enzyme activities in developing cotton fibers. Plant Physiol Biochem 32:697–702

    Google Scholar 

  • Weis KG, Jacobsen KR, Jernstedt JA (1999) Cytochemistry of developing cotton fibers: a hypothesized relationship between motes and non-dyeing fibers. Field Crops Res 62:107–117

    Article  Google Scholar 

  • Yang SS, Cheung F, Lee JJ et al (2006) Accumulation of genome-specific transcripts, transcription factors and phytohormonal regulators during early stages of fiber cell development in allotetraploid cotton. Plant J 47:761–775

    Article  CAS  Google Scholar 

  • Yang X, Tu L, Zhu L et al (2008) Expression profile analysis of genes involved in cell wall regeneration during protoplast culture in cotton by suppression subtractive hybridization and macroarray. J Exp Bot 59:3661–3674

    Article  PubMed  CAS  Google Scholar 

  • You-Ming Y, Chu-Nian X, Bio-Min W et al (2001) Effects of plant growth regulators on secondary wall thickening of cotton fibres. Plant Growth Regul 35:233–237

    Article  Google Scholar 

  • Zhang D, Hrmova M, Wan C-H et al (2004) Members of a new group of chitinase-like genes are expressed preferentially in cotton cells with secondary walls. Plant Mol Biol 54:353–372

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

For research support, we thank Cotton Incorporated, Cary, NC. The undergraduate research of H. Cheek was supported by the Honors Program of the College of Agriculture and Life Sciences and the Undergraduate Research Program of North Carolina State University. We thank E. Taliercio, USDA/ARS, Raleigh, NC for contribution of primer sequences to amplify an invertase gene with enhanced expression at the primary wall stage.

Author information

Authors and Affiliations

  1. Department of Crop Science, North Carolina State University, Campus Box 7620, Raleigh, NC, 27695, USA

    Bir Singh, Hannah D. Cheek & Candace H. Haigler

  2. Department of Plant Biology, North Carolina State University, Campus Box 7620, Raleigh, NC, 27695, USA

    Bir Singh, Hannah D. Cheek & Candace H. Haigler

Authors
  1. Bir Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hannah D. Cheek
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Candace H. Haigler
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Candace H. Haigler.

Additional information

Communicated by Y. Lu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Singh, B., Cheek, H.D. & Haigler, C.H. A synthetic auxin (NAA) suppresses secondary wall cellulose synthesis and enhances elongation in cultured cotton fiber. Plant Cell Rep 28, 1023–1032 (2009). https://doi.org/10.1007/s00299-009-0714-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00299-009-0714-2

Keywords

Search

Navigation