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Agrobacterium-mediated transformation of apricot (Prunus armeniaca L.) leaf explants

  • Genetic Transformation and Hybridization
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Abstract

A protocol for Agrobacterium-mediated stable transformation for scored, whole leaf explants of the apricot (Prunus armeniaca) cultivar Helena was developed. Regenerated shoots were selected using a two-step increased concentrations of paromomycin sulphate. Different factors affecting survival of transformed buds, including possible toxicity of green fluorescent protein (GFP) and time of exposure to high cytokine concentration in the regeneration medium, were examined. Transformation efficiency, based on PCR analysis of individual putative transformed shoots from independent lines was 5.6%, when optimal conditions for bud survival were provided. Southern blot analysis on four randomly chosen PCR-positive shoots confirmed the presence of the nptII transgene. This is the first time that stable transformation of an apricot cultivar is reported and constitutes also one of the few reports on the transformation of Prunus cultivars.

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Abbreviations

2,4-d:

2,4-Dichlorophenoxy-acetic acid

BA:

N 6-Benzylamino-purine

DKW:

Driver and Kuniyuki (1984) basal salt medium

GFP:

Green fluorescent protein

GUS:

β-Glucuronidase

IBA:

Indole-3-butyric acid

NAA:

α-Naphthalene acetic acid

QL:

Quoirin and Lepoivre (1977) basal salt medium

STS:

Silver thiosulphate

TDZ:

Thidiazuron

PAR:

Paromomycin sulphate

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Acknowledgments

The Authors wish to thank the financial support for this work provided by the CICYT projects AGL2005–01628 and AGL2006–0174. N. Alburquerque and M. Faize were supported by a postdoctoral contract “Juan de la Cierva” and “Ramón & Cajal”, respectively, from the Spanish Ministry of Education.

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Authors and Affiliations

  1. Mejora de Plantas, CEBAS-CSIC, Aptd. de Correos 164, 30100, Murcia, Spain

    César Petri, Hong Wang, Nuria Alburquerque, Mohamed Faize & Lorenzo Burgos

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  1. César Petri
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  2. Hong Wang
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  3. Nuria Alburquerque
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  4. Mohamed Faize
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  5. Lorenzo Burgos
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Corresponding author

Correspondence to Lorenzo Burgos.

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Communicated by H. Ebinuma.

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Petri, C., Wang, H., Alburquerque, N. et al. Agrobacterium-mediated transformation of apricot (Prunus armeniaca L.) leaf explants. Plant Cell Rep 27, 1317–1324 (2008). https://doi.org/10.1007/s00299-008-0550-9

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  • DOI: https://doi.org/10.1007/s00299-008-0550-9

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