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Use of green fluorescent protein for detection of transformed shoots and homozygous offspring

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Abstract

 Using tobacco as a model system, the data obtained demonstrated that the green fluorescent protein (GFP) can be used as a visual selection marker for transformed tissues. Based on differences in the intensity of GFP fluorescence, homozygous and hemizygous states could be easily visualized in seeds and seedlings of the T1 generation. These results were confirmed by genetic analysis. Optimized conditions for GFP analysis of stable transformants are presented.

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Authors and Affiliations

  1. Institut de Biologie Moléculaire des Plantes du CNRS et Université Louis Pasteur, 12, rue du Général Zimmer, F-67084 Strasbourg Cedex, France e-mail: Gunther.Hahne@ibmp-ulp.u-strasbg.fr, Fax: +33-3-88614442, , , , , , FR

    J. Molinier, C. Himber & G. Hahne

Authors
  1. J. Molinier
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  2. C. Himber
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  3. G. Hahne
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Received: 9 February 1999 / Revision received: 26 April 1999 / Accepted: 19 May 1999

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Molinier, J., Himber, C. & Hahne, G. Use of green fluorescent protein for detection of transformed shoots and homozygous offspring. Plant Cell Reports 19, 219–223 (2000). https://doi.org/10.1007/s002990050002

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  • DOI: https://doi.org/10.1007/s002990050002

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