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Introduction and establishment of apricot in vitro through regeneration of shoots from meristem tips

  • Micropropagation
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Summary

In this study different aspects of the in vitro introduction and establishment of apricot cultivars were investigated through meristem tip culture. The best time to introduce the meristems of ‘Canino’ was when buds were starting to swell. Various plant growth regulators were used at different concentrations on four distinct apricot cultivars to promote the development of the meristems to shoots which could then be micropropagated. Very diverse results were obtained depending on the genotype. In general, meristems did not survive without N6-benzyladenine. Concentrations of gibberellic acid from 2 to 4 mg 1−1 (5.8–11.4 µM) promoted explant elongation. This step was critically important to obtain apricot shoots large enough to be transferred to proliferation medium.

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References

  • Boxus, P. The production of strawberry plants by in vitro micropropagation. J. Hortic. Sci. 49:209–210; 1974.

    CAS  Google Scholar 

  • Boxus, P.; Druart, P. Virus-free through tissue culture. In: Bajaj, Y. P. S., ed. Biotechnology in agriculture and forestry. Trees I. Vol. 1. Berlin Heidelberg New York: Springer-Verlag; 1986:24–30.

    Google Scholar 

  • Boxus, P.; Quoirin, M. La culture de méristèmes apicaux de quelques especes de Prunus. Bull. Soc. Roy. Bot. Belg. 107:91–101; 1974.

    CAS  Google Scholar 

  • Campbell, R. Plant microbiology. London: Arnold; 1985.

    Google Scholar 

  • Carré, M.; Martin-Tanguy, J.; Mussillon, P.; Martin, C. La culture de méristèmes et la multiplication végétative in vitro au service de la pépinière. Université de Dijon; 1978.

  • Cassells, A. C. Problems in tissue culture: culture contamination. In: Debergh, P. C.; Zimmerman, R. H., ed. Micropropagation. Dordrecht, The Netherlands: Kluwer Academic Publishers; 1991:31–44.

    Google Scholar 

  • George, E. F. Plant propagation by tissue culture (Part 1 and 2). Edington: Exegetics Ltd.; 1993.

    Google Scholar 

  • Hartmann, H. T.; Kester, D. E. Plant propagation, principles and practices. 3rd ed. New York: Prentice-Hall Englewood Cliffs; 1975.

    Google Scholar 

  • Lane, W. D. Regeneration of apple plants from shoot meristem-tips. Plant Sci. Lett. 13:281–285; 1978.

    Article  CAS  Google Scholar 

  • Leifert, C.; Waites, W. M. Contaminants of plant tissue cultures. In: Newsletter No. 60, International Assoc. Plant. Tissue Cult.; 1990:2–13.

  • Marino, G.; Bertazza, G.; Magnanini, E.; Altan, A. D. Comparative effects of sorbitol and sucrose as main carbon energy sources in micropropagation of apricot. Plant Cell Tissue Organ Cult. 34:235–244; 1993.

    Article  CAS  Google Scholar 

  • Quoirin, M.; Lepoivre, P. Etude de milieux adaptes aux cultures in vitro de Prunus. Acta Hortic. (Wageningen) 78:437–442; 1977.

    Google Scholar 

  • SAS Institute, Inc. SAS/STAT User’s Guide. Release 6.03. SAS Institute, Inc. Cary, NC; 1988.

    Google Scholar 

  • Skirvin, R. M.; Chu, M. C.; Rukan, H. Tissue culture of peach, sweet and sour cherry, and apricot shoot tips. Proceedings of the 124th Annual Meeting of the Illinois State Horticultural Society 113:30–38; 1979.

  • Snir, I. In vitro propagation of sweet cherry cultivars. HortScience 17:192–193; 1982.

    CAS  Google Scholar 

  • Snir, I. In vitro propagation of ‘Canino’ apricot. HortScience 19:229–230; 1984.

    Google Scholar 

  • Zilkah, S.; Paingersh, E.; Rotbaum, A. In vitro propagation of three M × M (Prunus avium × Prunus mahaleb) cherry rootstocks. Acta Hortic. (Wageningen) 314:93–97; 1992.

    Google Scholar 

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Correspondence to L. Burgos.

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Pérez-Tornero, O., Burgos, L. & Egea, J. Introduction and establishment of apricot in vitro through regeneration of shoots from meristem tips. In Vitro Cell.Dev.Biol.-Plant 35, 249–253 (1999). https://doi.org/10.1007/s11627-999-0087-9

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  • DOI: https://doi.org/10.1007/s11627-999-0087-9

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