Abstract
Seedlings of 12 crosses involving pear varieties or hybrids were observed for the presence of haploid plants. On the basis of phenotypic characteristics, 17 plants corresponded to the haploid condition and, of these, 12 were determined by chromosome counting to be haploid (2n=x=17). In addition, and in order to induce in situ parthenogenesis, several pear varieties were pollinated with a selected clone carrying a homozygous dominant marker gene for the colour of red. This pollen had previously been irradiated with γ-rays of cobalt 60 at 0, 200, 250 and 500 Grays. The immature embryos were cultured in vitro, whereby 1 haploid and two mixoploid plants were obtained. Numerous diploid plants with the maternal phenotype were also obtained, and their genetic origin was subsequently studied by means of isozyme analysis.
Similar content being viewed by others
References
Braniste N, Popescu A, Coman T (1984) Producing and multiplication of Pyrus communis haploid plants. Acta Hortic 161:147–150
Calzoni GL, Sperenza A, Bagni N (1979) In vitro germination of apple pollen. Sci Hortic 10:49–55
Carreau P (1988) Parthénogenèse induite par du pollen irradié chez le pommier (Malus Xdomestica Borkh): germination et croissance des tubes polliniques, évolution de l'ovule. Mémoire de D.E.A., Université de Rennes I
Chase SS (1969) Monoploids and monoploid-derived maize. Bot Rev 35:117–167
Chevreau E, Laurens F (1987) The pattern inheritance in apple (Malus x domestica Borkh.): further results from leaf isozyme analysis. Theor Appl Genet 75:90–95
Decourtye L (1967) Etude de quelques caractères à contrôle génétique simple chez le pommier (Malus sp.) et le poirier (Pyrus communis). Ann Amelior Plant 17:243–266
Fei FW, Xue GR (1981) Induction of haploid plantlets by anther culture in vitro in apple cv ‘Delicious’. Chin Agric Sci 4:41–44 (in Chinese, English abstract)
Fleckinger J (1964) Le bon jardinier. In: Grisvard P, Chaudun V (eds) La maison rustique. Paris, pp 362–372
Hidano Y (1982) Callus and embryoid induction by anther culture in apple. Bull Fac Educ Hirosaki Univ 48:69–74
Jordan M (1975) In vitro culture of anther from Prunus, Pyrus and Ribes, Planta Med Suppl pp 59–65
Kubicki B, Telezynska J, Milewska-Pawliczuk E (1975) Induction of embryoid development from apple pollen grains. Acta Soc Bot Pol 44:631–635
Lashermes P, Beckert M (1988) Genetic control of maternal haploidy in maize (Zea mays L.) and selection of haploid inducing lines. Theor Appl Genet 76:405–410
Lecuyer MP, Zhang YX, Tellier M, Lespinasse Y (1991) In vitro pollen tube division of irradiated and non-irradiated apple pollen. Agronomie 11:483–489
Lespinasse Y, Salesses G (1973) Application de techniques nouvelles à l'observation des chromosomes chez les genres Malus et Pyrus. Ann Amelior Plant 23:381–386
Mogensen HL (1982) Double fertilisation in barley and the cytological explanation for haploid embryo formation, embryoless caryopses, and ovule abortion. Carlsberg Res Commun 47:313–354
Quoirin M, Lepoivre P, Boxus P (1977) Un premier bilan de dix années de recherche sur les cultures de méristèmes et la multiplication in vitro de fruitiers ligneux. C R Rech Stn Fruit maraichères, Gembloux, pp 93–117
Sniezko R, Visser T (1987) Embryo development and fruit-set in pear induced by untreated and irradiated pollen. Euphytica 36:287–294
Solntzeva M (1987) Apomixis and hemigamy as one of its forms. Proc Indian Natl Sci Acad B44:78–90
Xue GR, Niu JZ (1984) A study on the induction of apple pollen plants. Acta Hortic Sin 11:161–164 (in Chinese, English abstract)
Zhang YX, Lespinasse Y (1991) Pollination with gamma-irradiated pollen and development of fruits seeds and parthenogenetic plants in apple. Euphytica 54:101–109
Zhang YX, Lespinasse Y (1992) Haploidy. In: Hammerschlag F, Litz RE (eds) Biotechnology of perennial fruit crops. CAB Int. Wallingford, UK, pp 57–75
Zhang YX, Boccon-Gibod J, Lespinasse Y (1987) Obtention d'embryons de pommier (Malus Xdomestica Borkh) après culture d'anthères. C R Acad Sci Paris Ser III 305:443–448
Zhang YX, Lespinasse Y, Chevreau E (1990) Induction of haploidy in fruit trees. Acta Hortic 280:293–305
Zverzhanskaya LS, Grishina EV, Komarova PI (1980) Cytoembryological study of haploidy and polyembryony in restitution lines of corn. Tsitol Genet 14:3–9
Author information
Authors and Affiliations
Additional information
Communicated by G. Wenzel
Rights and permissions
About this article
Cite this article
Bouvier, L., Zhang, YX. & Lespinasse, Y. Two methods of haploidization in pear, Pyrus communis L.: greenhouse seedling selection and in situ parthenogenesis induced by irradiated pollen. Theoret. Appl. Genetics 87, 229–232 (1993). https://doi.org/10.1007/BF00223769
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00223769