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Abiotic stress response in the moss Physcomitrella patens: evidence for an evolutionary alteration in signaling pathways in land plants

  • Biotic and Abiotic Stress
  • Published:
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Abstract

The mechanisms plants use to adapt to abiotic stress have been widely studied in a number of seed plants. Major research has been focused on the isolation of stress-responsive genes as a means to understand the molecular events underlying the adaptation process. To study stress-related gene regulation in the moss Physcomitrella patens we have isolated two cDNAs showing homology to highly conserved small hydrophobic proteins from different seed plants. The corresponding genes are up-regulated by dehydration, salt, sorbitol, cold and the hormone abscisic acid, indicating overlapping pathways are involved in the control of these genes. Based on the molecular characterization of the moss homologs we propose that signaling pathways in response to abiotic stress may have been altered during the evolution of land plants.

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Abbreviations

ABA :

Abscisic acid

EST :

Expressed sequence tag

References

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    Article  CAS  PubMed  Google Scholar 

  • Bartels D, Salamini F (2001) Desiccation tolerance in the resurrection plant Craterostigma plantagineum. A contribution to the study of drought tolerance at the molecular level. Plant Physiol 127:1346–1353

    CAS  PubMed  Google Scholar 

  • Bartels D, Chandler J, Bockel C, Frank W, Kleines M, Rodrigo MJ, Phillips J, Mariaux JB, Furini A, Salamini F (1997) Investigating the molecular basis of desiccation tolerance using the resurrection plant Craterostigma plantagineum as an experimental system. Acta Physiol Plant 19:399–403

    CAS  Google Scholar 

  • Bezanilla M, Pan A, Quatrano RS (2003) RNA interference in the moss Physcomitrella patens. Plant Physiol 133:470–474

    Article  CAS  PubMed  Google Scholar 

  • Blumwald E (2000) Sodium transport and salt tolerance in plants. Curr Opin Cell Biol 12:431–434

    Google Scholar 

  • Bohnert H, Cushman J (2000) The ice plant cometh: lessons in abiotic stress tolerance. J Plant Growth Regul 19:334–346

    CAS  Google Scholar 

  • Brown A (1998) A biomolecular analysis of the control of expression and function of a low temperature responsive barley gene. PhD thesis, University of Newcastle upon Tyne, UK

  • Capel J, Jarillo JA, Salinas J, Martinez-Zapater JM (1997) Two homologous low-temperature-inducible genes from Arabidopsis encode highly hydrophobic proteins. Plant Physiol 115:569–576

    Article  CAS  PubMed  Google Scholar 

  • Goddard NJ, Dunn MA, Zhang L, White AJ, Jack PL, Hughes MA (1993) Molecular analysis and spatial expression pattern of a low-temperature-specific barley gene, blt101. Plant Mol Biol 23:871–879

    CAS  PubMed  Google Scholar 

  • Gulick PJ, Shen W, An H (1994) ESI3, a stress-induced gene from Lophopyrum elongatum. Plant Physiol 104:799–800

    Article  CAS  PubMed  Google Scholar 

  • Jonak C, Kiegerl S, Ligterink W, Barker PJ, Huskisson NS, Hirt H (1996) Stress signaling in plants: a mitogen-activated protein kinase pathway is activated by cold and drought. Proc Natl Acad Sci USA 93:11274–11279

    Article  CAS  PubMed  Google Scholar 

  • Knight H, Knight MR (2001) Abiotic stress signalling pathways: specificity and cross-talk. Trends Plant Sci 6:262–267

    Article  CAS  PubMed  Google Scholar 

  • Kovtun Y, Chiu WL, Tena G, Sheen J (2000) Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants. Proc Natl Acad Sci USA 97:2940–2945

    CAS  PubMed  Google Scholar 

  • Kyte J, Doolittle RF (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157:105–132

    CAS  PubMed  Google Scholar 

  • Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K, Shinozaki K (1998) Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell 10:1391–1406

    CAS  PubMed  Google Scholar 

  • Mizoguchi T, Irie K, Hirayama T, Hayashida N, Yamaguchi-Shinozaki K, Matsumoto K, Shinozaki K (1996) A gene encoding a mitogen-activated protein kinase is induced simultaneously with genes for a mitogen-activated protein kinase and an S6 ribosomal protein kinase by touch, cold, and water stress in Arabidopsis thaliana. Proc Natl Acad Sci USA 93:765–769

    Article  CAS  PubMed  Google Scholar 

  • Nylander M, Heino P, Helenius E, Palva ET, Ronne H, Welin BV (2001) The low-temperature- and salt-induced RCI2A gene of Arabidopsis complements the sodium sensitivity caused by a deletion of the homologous yeast gene SNA1. Plant Mol Biol 45:341–352

    Article  CAS  PubMed  Google Scholar 

  • Oliver MJ, Velten J, Wood AJ (2000) Bryophytes as experimental models for the study of environmental stress tolerance: Tortula ruralis and desiccation-tolerance in mosses. Plant Ecol 151:73–84

    Article  Google Scholar 

  • Pawlowski K, Kunze R, de Vries S, Bisseling T (1994) Isolation of total, poly(A) and polysomal RNA from plant tissues. In: Gelvin SB, Schilperoort RA (eds) Plant molecular biology. Kluwer, Dordrecht. pp 29–44

  • Persson B, Argos P (1994) Prediction of transmembrane segments in proteins utilising multiple sequence alignments. J Mol Biol 237:182–192

    CAS  PubMed  Google Scholar 

  • Persson B, Argos P (1996) Topology prediction of membrane proteins. Protein Sci 5:363–371

    CAS  PubMed  Google Scholar 

  • Rensing SA, Rombauts S, Van de Peer Y, Reski R (2002) Moss transcriptome and beyond. Trends Plant Sci 7:535–538

    Article  CAS  PubMed  Google Scholar 

  • Reski R, Faust M, Wang XH, Wehe M, Abel WO (1994) Genome analysis of the moss Physcomitrella patens (Hedw.) B.S.G. Mol Gen Genet 244:352–359

    CAS  PubMed  Google Scholar 

  • Sambrook J, Russel DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor

    Google Scholar 

  • Seki M, Kamei A, Yamaguchi-Shinozaki K, Shinozaki K (2003) Molecular responses to drought, salinity and frost: common and different paths for plant protection. Curr Opin Biotechnol 14:194–199

    Article  CAS  PubMed  Google Scholar 

  • Shinozaki K, Dennis ES (2003) Cell signalling and gene regulation: global analyses of signal transduction and gene expression profiles. Curr Opin Plant Biol 6:405–409

    Article  CAS  PubMed  Google Scholar 

  • Shinozaki K, Yamaguchi-Shinozaki K (2000) Molecular responses to dehydration and low temperature: differences and cross-talk between two stress signaling pathways. Curr Opin Plant Biol 3:217–223

    CAS  PubMed  Google Scholar 

  • Shinozaki K, Yamaguchi-Shinozaki K, Seki M (2003) Regulatory network of gene expression in the drought and cold stress responses. Curr Opin Plant Biol 6:410–417

    Article  CAS  PubMed  Google Scholar 

  • Strepp R, Scholz S, Kruse S, Speth V, Reski R (1998) Plant nuclear gene knockout reveals a role in plastid division for the homolog of the bacterial cell division protein FtsZ, an ancestral tubulin. Proc Natl Acad Sci USA 95:4368–4373

    CAS  PubMed  Google Scholar 

  • Theissen G, Muenster T, Henschel K (2001) Why don’t mosses flower? New Phytol 150:1–5

    Article  CAS  Google Scholar 

  • Thomashow MF (2001) So what’s new in the field of plant cold acclimation? Lots! Plant Physiol 125:89–93

    Article  CAS  Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    PubMed  Google Scholar 

  • Wood AJ, Oliver MJ, Cove DJ (2000) Bryophytes as model systems. Bryologist 103:128–133

    Google Scholar 

  • Xiong L, Zhu JK (2001) Abiotic stress signal transduction in plants: molecular and genetic perspectives. Physiol Plant 112:152–166

    Article  CAS  PubMed  Google Scholar 

  • Xiong L, Ishitani M, Zhu JK (1999) Interaction of osmotic stress, temperature, and abscisic acid in the regulation of gene expression in Arabidopsis. Plant Physiol 119:205–212

    Article  CAS  PubMed  Google Scholar 

  • Zhu JK (2003) Regulation of ion homeostasis under salt stress. Curr Opin Plant Biol 6:441–445

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. University of Freiburg, Schaenzlestr. 1, 79104, Freiburg, Germany

    K. Kroemer, R. Reski & W. Frank

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  1. K. Kroemer
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  2. R. Reski
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  3. W. Frank
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Correspondence to W. Frank.

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Communicated by D. Bartels

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Kroemer, K., Reski, R. & Frank, W. Abiotic stress response in the moss Physcomitrella patens: evidence for an evolutionary alteration in signaling pathways in land plants. Plant Cell Rep 22, 864–870 (2004). https://doi.org/10.1007/s00299-004-0785-z

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  • DOI: https://doi.org/10.1007/s00299-004-0785-z

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