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Induction of male sterile cabbage using a tapetum-specific promoter from Brassica campestris L. ssp. pekinensis

  • Genetic Transformation and Hybridization
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Abstract

The anther (tapetum)-specific gene BcA9 was isolated from Chinese cabbage, Brassica campestris L. ssp. pekinensis cv. Jangwon, using the Arabidopsis tapetum-specific A9 gene as a probe. The DNA and amino acid sequences of the coding region of the BcA9 gene showed high homology with A9 genes from Arabidopsis and B. napus. However, the DNA sequences of the 5′ noncoding (promoter) region were different, except for the sequence from −281 to −89. To test the specific activity of this promoter, a plant expression vector, pGR011, was constructed by fusing the BcA9 promoter and the cytotoxic diphtheria toxin A-chain (DTx-A) gene. Several transgenic plants from cabbage, B. oleracea ssp. capitata, were obtained by way of Agrobacterium-mediated transformation. Southern blot analysis indicated that the tapetum-specific BcA9 promoter and DTx-A gene were successfully integrated into the genome of the transgenic cabbage. Under the control of the BcA9 promoter, expression of the cytotoxic DTx-A gene in the tapetal cells of the transgenic plants resulted in male sterile cabbages. Microscopic examination revealed that pollen grains in anthers of the male sterile cabbages had not developed normally, but the vegetative growth and phenotype showed no difference compared to wild-type plants.

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Abbreviations

At :

Arabidopsis thaliana

Bc :

Brassica camepstris

Bn :

Brassica napus

DTx-A :

Diphtheria toxin A-chain gene

hpt :

Hygromycin phosphotransferase

PCR:

Polymerase chain reaction

SDS:

Sodium dodecyl sulfate

SSC:

Sodium chloride-sodium citrate buffer

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Authors and Affiliations

  1. National Institute of Agricultural Biotechnology, RDA, 441-707, Suwon, Korea

    Y.-H. Lee, H.-U. Kim, Y.-M. Jin, H.-I. Kim & B.-S. Park

  2. Department of Horticultural Science, Chung-Ang University, 456-756, Ansung, Korea

    K.-H. Chung

Authors
  1. Y.-H. Lee
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  2. K.-H. Chung
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  3. H.-U. Kim
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  4. Y.-M. Jin
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  5. H.-I. Kim
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  6. B.-S. Park
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Corresponding author

Correspondence to B.-S. Park.

Additional information

Communicated by I.S. Chung

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Lee, YH., Chung, KH., Kim, HU. et al. Induction of male sterile cabbage using a tapetum-specific promoter from Brassica campestris L. ssp. pekinensis . Plant Cell Rep 22, 268–273 (2003). https://doi.org/10.1007/s00299-003-0688-4

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  • DOI: https://doi.org/10.1007/s00299-003-0688-4

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