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Rice ubiquitin promoters: deletion analysis and potential usefulness in plant transformation systems

  • Genetic Transformation and Hybridization
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Abstract

Strong constitutive promoters form a cornerstone for basic and applied research using transgenic plants. GUS (β-glucuronidase) expression levels from constructs containing RUBQ1 or RUB2 rice ubiquitin promoters were 8- to 35-fold higher in transgenic rice [Oryza sativa (L.)] plants, respectively, when compared to the 35S promoter. Deletion analysis of the 5′-upstream region of RUBQ2 revealed a putative enhancer region that produced a 2.4-fold increase in transient GUS expression. Southern blot analysis showed that three to seven copies of the GUS gene were stably inserted into R0 and R1 plants and inherited in a monogenic fashion.

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Fig. 1A, B.
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Abbreviations

BAR :

Native phosphinothricin acetyltransferase

GUS :

β-Glucuronidase

HPH :

Hygromycin phosphotransferase

LUC :

Luciferase

PAT :

Synthetic phosphinothricin acetyltransferase

PCR :

Polymerase chain reaction

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Acknowledgements

We thank Dr. Peter Quail (USDA Plant Gene Expression Center, Albany, Calif.) for plasmid pAHC25, and Dr. M.C. Rush, Department of Plant Pathology and Crop Physiology, Louisiana State University, Baton Rouge, La., for Taipei 309 suspension cells. This research was funded in part by the Louisiana Rice Research Board.

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Author notes

  1. J. Wang

    Present address: Genetics Programm and Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843-2474, USA

Authors and Affiliations

  1. Department of Agronomy, AgCenter Biotechnology Laboratory, Louisiana Agricultural Experiment Station, LSU Agricultural Center, Louisiana State University, Baton Rouge, LA, 70803, USA

    J. Wang & J. H. Oard

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  1. J. Wang
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  2. J. H. Oard
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Correspondence to J. H. Oard.

Additional information

Communicated by K.K. Kamo

Approved for publication by the Director of the Louisiana Agricultural Experiment Station as manuscript number 02-14-0709.

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Wang, J., Oard, J.H. Rice ubiquitin promoters: deletion analysis and potential usefulness in plant transformation systems. Plant Cell Rep 22, 129–134 (2003). https://doi.org/10.1007/s00299-003-0657-y

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  • DOI: https://doi.org/10.1007/s00299-003-0657-y

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