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Isolation and Genome Sequence of a Novel Phosphate-Solubilizing Rhizobacterium Bacillus altitudinis GQYP101 and Its Effects on Rhizosphere Microbial Community Structure and Functional Traits of Corn Seedling

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Abstract

Bacillus altitudinis is a widely distributed soil bacterium that has various functional activities, including remediation of contaminated soil, degradation of herbicides, and enhancement of plant growth. B. altitudinis GQYP101 was isolated from the rhizosphere soil of Lycium barbarum L. and demonstrated potential as a plant growth-promoting bacterium. In this work, strain GQYP101 could solubilize phosphorus, and increased the stem diameter, maximum leaf area, and fresh weight of corn in a pot experiment. Nitrogen and phosphorus contents of corn seedlings (aerial part) increased by 100% and 47.9%, respectively, after application of strain GQYP101. Concurrently, nitrogen and phosphorus contents of corn root also increased, by 55.40% and 20.3%, respectively. Furthermore, rhizosphere soil nutrients were altered and the content of available phosphorus increased by 73.2% after application of strain GQYP101. The mechanism by which strain GQYP101 improved plant growth was further investigated by whole genome sequence analysis. Strain GQYP101 comprises a circular chromosome and a linear plasmid. Some key genes of strain GQYP101 were identified that were related to phosphate solubilization, alkaline phosphatase, chemotaxis, and motility. The findings of this study may provide a theoretical basis for strain GQYP101 to enhance crop yield as microbial fertilizer.

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Funding

The National Natural Science Foundation of China (31700094) supported Dr. Chengqiang Wang. The National Natural Science Foundation of China (31770115) supported Dr. Yanqin Ding. The National Key Research and Development Program of China (No. 2017YFD0200804), the Science and Technology Project of Guizhou (ZYJ2017-8), and the Key Field Research and Development Program of Guangdong Province (2019B020218009) supported Prof. Binghai Du.

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  1. Dongying Zhao and Yanqin Ding contributed equally to this article.

Authors and Affiliations

  1. College of Life Sciences and Shandong Engineering Research Center of Plant, Microbia Restoration for Saline-Alkali Land and Shandong Key Laboratory of Agricultural Microbiology and National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural University, Taian, China

    Dongying Zhao, Yanqin Ding, Yanru Cui, Yanan Zhang, Kai Liu, Liangtong Yao, Binghai Du & Chengqiang Wang

  2. Zunyi Tobacco Monopoly Administration of Guizhou, Zunyi, China

    Xiaobin Han, Yulong Peng & Jianyu Gou

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Contributions

DZ and YC performed the work and analyzed the data. DZ and YD wrote the original draft. CW revised the manuscript. YZ, KL, LY, XH, YP, and JG advised the manuscript. CW and BD supported the study.

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Correspondence to Chengqiang Wang.

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Zhao, D., Ding, Y., Cui, Y. et al. Isolation and Genome Sequence of a Novel Phosphate-Solubilizing Rhizobacterium Bacillus altitudinis GQYP101 and Its Effects on Rhizosphere Microbial Community Structure and Functional Traits of Corn Seedling. Curr Microbiol 79, 249 (2022). https://doi.org/10.1007/s00284-022-02944-z

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