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Genetic Diversity and Characterization of Plant Growth-Promoting Effects of Bacteria Isolated from Rhizospheric Soils

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Plant growth-promoting rhizobacteria (PGPR) have the potential to make a significant contribution to the development of sustainable agricultural systems. Generally, PGPRs function in three different ways, summarized as the synthesis of certain compounds for plants, facilitating the uptake of certain nutrients from the soil and protecting plants from diseases. This study aims to isolate plant growth-promoting bacteria from different plant rhizospheres from Ankara province, to reveal their genetic diversity, and to determine their plant growth-promoting properties. The identification of the 69 isolates was made according to the 16S rDNA sequence results and ARDRA analyses were also performed using AluI, HeaIII, and MspI enzymes. Nitrogen fixation, phosphate dissolving, IAA (indole-3-acetic acid) and siderophore production capacities of the 69 bacterial strains including 12 different genera (30 Pseudomonas, 13 Arthrobacter, 7 Bacillus, 4 Phyllobacter, 4 Variovorax, 3 Olivibacter, 3 Enterobacter, 2 Paenarthrobacter, 1 Stenotrophomonas, 1 Flavobacterium, 1 Caulobacter, 1 Paenibacillus) were evaluated in in vitro conditions. Nitrogen fixation capacities of 55 isolates varied between 2.29 and 46.11 µg mL−1 according to micro-kjeldahl method. Among the strains studied, nifH gene was detected only in Paenibacillus polymyxa H8/2 strain. The highest Phosphorus dissolving and IAA production capacity (in tryptophan-added medium) of isolates were  186.52 µg mL−1, and 50.05 μg mL−1  respectively, and 31 of 69 isolates were able to produce siderophore. Regarding antifungal activities, results showed that 31 bacterial isolates had antagonistic activities against at least one of the tested pathogens. Nitrogen fixation and phosphate solubilizing potential of the promising bacterial strains were determined through two-independent pot experiments with wheat and it has been found that they have positive effects on the yield parameters of wheat.

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Authors and Affiliations

  1. Soil Fertilizer and Water Resources Center Research Institute, Ankara, Turkey

    Dilek Kaya Özdoğan

  2. Biotechnology Institute, Ankara University, Ankara, Turkey

    Nefise Akçelik

  3. Faculty of Science, Department of Biology, Ankara University, Ankara, Turkey

    Mustafa Akçelik

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  1. Dilek Kaya Özdoğan
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Özdoğan, D.K., Akçelik, N. & Akçelik, M. Genetic Diversity and Characterization of Plant Growth-Promoting Effects of Bacteria Isolated from Rhizospheric Soils. Curr Microbiol 79, 132 (2022). https://doi.org/10.1007/s00284-022-02827-3

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