Abstract
In recent years, plant growth-promoting rhizobacteria (PGPR) have received increased attention due to their prospective use as biofertilizers for the enhancement of crop growth and yields. However, there is a growing need to identify new PGPR isolates with additional beneficial properties. In this paper, we describe the identification of a new strain of a non-sporulating Gram-positive bacterium isolated from the rhizosphere of potato plants, classified as Brevibacterium sediminis MG-1 based on whole-genome sequencing. The bacteria are aerobic; they grow in a pH range of 6.0–10.0 (optimum 6.0), and a temperature range of 20–37 °C (optimum 30 °C). At 96 h of cultivation, strain MG-1 synthesizes 28.65 µg/ml of indole-3-acetic acid (IAA) when 500 µg/ml of l-tryptophan is added. It is a producer of catechol-type siderophores and ACC deaminase (213 ± 12.34 ng/ml) and shows halotolerance. Treatment of pea, rye, and wheat seeds with a suspension of MG-1 strain cells resulted in the stimulation of stem and root biomass accumulation by 12–26% and 6–25% (P < 0.05), respectively. Treatment of seeds with bacteria in the presence of high salt concentration reduced the negative effects of salt stress on plant growth by 18–50%. The hypothetical gene lin, encoding the bacteriocin Linocin-M18, RIPP-like proteins, and polyketide synthase type III (T3PKS) loci, gene clusters responsible for iron acquisition and metabolism of siderophores, as well as gene clusters responsible for auxin biosynthesis, were identified in the B. sediminis MG-1 genome. Thus, the rhizosphere-associated strain B. sediminis MG-1 has growth-stimulating properties and can be useful for the treatment of plants grown on soils with high salinity.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Data availability
All datasets generated for this study have been included in the article/Supplementary Information.
References
Ahmad F, Ahmad I, Khan MS (2008) Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiol Res 163:173–181. https://doi.org/10.1016/j.micres.2006.04.001
Anast JM, Dzieciol M, Schultz DL, Wagner M, Mann E, Schmitz-Esser S (2019) Brevibacterium from Austrian hard cheese harbor a putative histamine catabolism pathway and a plasmid for adaptation to the cheese environment. Sci Rep 9:1–12. https://doi.org/10.1038/s41598-019-42525-y
Angiuoli SV, Gussman A, Klimke W, Cochrane G, Field D, Garrity G (2008) Toward an online repository of standard operating procedures (SOPs) for (meta)genomic annotation. OMICS 12:137–141. https://doi.org/10.1089/omi.2008.0017
Arndt D, Grant JR, Marcu A, Sajed T, Pon A, Liang Y et al (2016) PHASTER: a better, faster version of the PHAST phage search tool. Nucleic Acids Res 44:16–21. https://doi.org/10.1093/nar/gkw387
Arnow LE (1937) Colorimetric determination of the components of 3, 4- dihydroxyphenylalanine-tyrosine mixtures. J Biol Chem 118:531–537
Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA (2008) The RAST server: rapid annotations using subsystems technology. BMC Genomics. https://doi.org/10.1186/1471-2164-9-75
Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, Lesin MV, Nikolenko SI, Pham S, Prjibelski AD, Pyshkin AV, Sirotkin AV, Vyahhi N, Tesler G, Alekseyev MA, Pevzner PA (2012) SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol. https://doi.org/10.1089/cmb.2012.0021
Becker J, Rohles CM, Wittmann C (2018) Metabolically engineered Corynebacterium glutamicum for bio-based production of chemicals, fuels, materials, and healthcare products. Metab Eng 50:122–141. https://doi.org/10.1016/j.ymben.2018.07.008
Beneduzi A, Ambrosini A, Passaglia LMP (2012) Plant growth-promoting rhizobacteria (PGPR): their potential as antagonists and biocontrol agents. Genet Mol Biol 35:1044–1051. https://doi.org/10.1590/s1415-47572012000600020
Bolger AM, Lohse M, Usadel B (2014) Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30:2114–2120. https://doi.org/10.1093/bioinformatics/btu170
Bui E (2013) Soil salinity: a neglected factor in plant ecology and biogeography. J Arid Environ 92:14–25
Chen P, Zhang L, Wang J, Ruan J, Han X, Huang Y (2016) Brevibacterium sediminis sp. nov., isolated from deep-sea sediments from the Carlsberg and Southwest Indian Ridges. Int J Syst Evol Microbiol 66:5268–5274
Chen R, Wong HL, Burns BP (2019) New approaches to detect biosynthetic gene clusters in the environment. Medicines. https://doi.org/10.3390/medicines6010032
Choi K, Siddiqi MZ, Liu Q, Shafi M, Durrani Y, Lee S, Kang M, Im W (2018) Brevibacterium hankyongi sp. nov., isolated from compost. Int J Syst Evol Microbiol. https://doi.org/10.1099/ijsem.0.002886
Choi J, Choudhury RA, Park SY, Oh MM, Sa T (2021) Inoculation of ACC deaminase-producing Brevibacterium linens RS16 enhances tolerance against combined UV-B radiation and heat stresses in rice (Oryza sativa L.). Sustainability. https://doi.org/10.3390/su131810013
Chopra A, KumarVandana U, Rahi P, Satpute SK, Mazumder PB (2020) Plant growth promoting potential of Brevibacterium sediminis A6 isolated from the tea rhizosphere of Assam, India. Biocatal Agric Biotechnol. https://doi.org/10.1016/j.bcab.2020.101610
Cruz-Morales P, Ramos-Aboites H, Licona-Cassani C, Selem N, Mejia-Ponce P, Souza V, Barona-Gómez F (2017) Actinobacteria phylogenomics, selective isolation from an iron oligotrophic environment and siderophore functional characterization, unveil new desferrioxamine traits. FEMS Microbiol Ecol. https://doi.org/10.1093/femsec/fix086
Cui Y, Kang M, Woo S, Jin L, Kim K, Park J, Lee M, Lee S (2013) Brevibacterium daeguense sp nov., a nitrate-reducing bacterium isolated from a 4-chlorophenol enrichment culture. Int J Syst Evol Microbiol. https://doi.org/10.1099/ijs.0.038141-0
Dar D, Thomashow LS, Weller DM, Newman DK (2020) Global landscape of phenazine biosynthesis and biodegradation reveals species specific colonization patterns in agricultural soils and crop microbiomes. Elife. https://doi.org/10.1101/2020.06.05.136879
Daulay FR, Simarmata T (2021) Current status and prospect of halotolerant biofilm PGPR (plant growth promoting Rhizobacteria) as bioagent to increase crops growth on saline soils. IOP Conf Ser Earth Environ Sci. https://doi.org/10.1088/1755-1315/748/1/012042
Elsakhawy TA, Fetyan NAH, Ghazi AA (2019) The potential use of ectoine produced by a moderately halophilic bacteria Chromohalobacter salexigens KT989776 for enhancing germination and primary seedling of flax “Linum usitatissimum L”. under salinity conditions. Biotechnol J Int 23:1–12
Faisal M, Hasnain S (2006) Plant growth promotion by brevibacterium under chromium stress. Res J Bot 1:24–29
Fredrick C, Muthuri C, Ngamau K, Sinclair F (2015) Provenance variation in seed morphological characteristics, germination and early seedling growth of Faidherbia. J Hortic for 7:127–140
Glick BR, Penrose DM, Li JP (1998) A model for the lowering of plant ethylene concentrations by plant growth-promoting bacteria. J Theor Biol 190:63–68. https://doi.org/10.1006/jtbi.1997.0532
Gopalakrishnan S, Humayun P, Kiran BK, Kannan IG, Vidiy MS, Deepthi K, Rupela O (2011) Evaluation of bacteria isolated from rice rhizosphere for biological control of charcoal rot of sorghum caused by Macrophomina phaseolina (Tassi). Gold World J Microbiol Biotechnol 27:1313–1321. https://doi.org/10.1007/s11274-010-0579-0
Gordon SA, Weber RP (1951) Colorimetric estimation of indoleacetic acid. Plant Physiol 26:192–195. https://doi.org/10.1104/pp.26.1.192
Gu S, Wei Z, Shao Z, Friman VP, Cao K, Yang T, Kramer J, Wang X, Li M, Mei X, Xu Y, Shen Q, Kümmerli R, Jousset A (2020) Competition for iron drives phytopathogen control by natural rhizosphere microbiomes. Nat Microbiol 5:1002–1010. https://doi.org/10.1038/s41564-020-0719-8
Gurevich A, Saveliev V, Vyahhi N, Tesler G (2013) QUAST: quality assessment tool for genome assemblies. Bioinformatics. https://doi.org/10.1093/bioinformatics/btt086
Guyomarch F, Binet A, Dufossé L (2000) Characterization of Brevibacterium linens pigmentation using spectrocolorimetry. Int J Food Microbiol 57:201–210. https://doi.org/10.1016/s0168-1605(00)00252-x
Harris LA, Saint-Vincent PMB, Guo X, Hudson GA, DiCaprio AJ, Zhu L, Mitchell DA (2020) Reactivity-based screening for citrulline-containing natural products reveals a family of bacterial peptidyl arginine deiminases. ACS Chem Biol 15:3167–3175. https://doi.org/10.1021/acschembio.0c00685
Harwood CR, Mouillon JM, Pohl S, Arnau J (2018) Secondary metabolite production and the safety of industrially important members of the Bacillus subtilis group. FEMS Microbiol Rev 42:721–738. https://doi.org/10.1093/femsre/fuy028
Heel A, Jong A, Montalban-Lopez M, Jan K, Kuipers O (2013) BAGEL3: automated identification of genes encoding bacteriocins and (non-) bactericidal posttranslationally modified peptides. Nucleic Acids Res. https://doi.org/10.1093/nar/gkt391
Kim J, Srinivasan S, You T, Bang JJ, Park S, Lee SS (2013) Brevibacterium ammoniilyticum sp. nov., an ammonia-degrading bacterium isolated from sludge of a wastewater treatment plant. Int J Syst Evol Microbiol 63:1111–1118. https://doi.org/10.1099/ijs.0.039305-0
Lévesque S, Dufresne PJ, Soualhine H, Domingo M-C, Bekal S, Lefebvre B et al (2015) A side by side comparison of bruker biotyper and VITEK MS: utility of MALDI-TOF MS technology for microorganism identification in a public health reference laboratory. PLoS ONE. https://doi.org/10.1371/journal.pone.0144878
Levesque S, de Melo AG, Labrie SJ, Moineau S (2019a) Mobilome of Brevibacterium aurantiacum sheds light on its genetic diversity and its adaptation to smear-ripened cheeses. Front Microbiol 10:1270. https://doi.org/10.3389/fmicb.2019.01270
Levesque S, de Melo AG, Labrie SJ, Moineau S (2019b) Mobilome of Brevibacterium aurantiacum sheds light on its genetic diversity and its adaptation to smear-ripened cheeses. Front Microbiol. https://doi.org/10.3389/fmicb.2019.01270
Li F, Lu Q, Liao S, Tuo L, Liu S, Yang Q, Shen A, Sun C (2021) Schumannella soli sp. nov., a novel actinomycete isolated from mangrove soil by in situ cultivation. Antonie Van Leeuwenhoek 114(10):1657–1667
Liu W, Hou J, Wang Q, Ding L, Luo Y (2014) Isolation and characterization of plant growth-promoting rhizobacteria and their effects on phytoremediation of petroleum-contaminated saline-alkali soil. Chemosphere. https://doi.org/10.1016/j.chemosphere.2014.07.026
Lutfullin MT, Hadieva GF, Sharipova MR, Mardanova AM (2016) Characterization of growth-promoting activity of Pseudomonas putida strain MG-2. Res J Pharm Biol Chem Sci 7:1538–1542
Lutfullin M, Zaripova D, Moiseeva O, Vologin S, Mardanova A (2020) Characterization of bacterial communities of rhizosphere and rhizoplane of Early Zhukovsky potato. E3S Web Conferences. https://doi.org/10.1051/e3sconf/202022202050
Majeed A, Abbasi MK, Hameed S, Imran A, Rahim N (2015) Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion. Front Microbiol. https://doi.org/10.3389/fmicb.2015.00198
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual, vol 545. Cold Spring Harbour Laboratory, Cold Spring Harbour, New York. https://doi.org/10.1002/abio.370050118
Mapelli F, Marasco R, Rolli E, Barbato M, Cherif H, Guesmi A, Ouzari I, Daffonchio D, Borin S (2013) Potential for plant growth promotion of rhizobacteria associated with Salicornia growing in Tunisian hypersaline soils. Biomed Res Int 13:1–13. https://doi.org/10.1155/2013/248078
Mardanova A, Lutfullin M, Hadieva G, Akosah Y, Pudova D, Kabanov D, Shagimardanova E, Vankov P, Vologin S, Gogoleva N, Stasevski Z, Sharipova M (2019) Structure and variation of root-associated microbiomes of potato grown in alfisol. World J Microbiol Biotechnol. https://doi.org/10.1007/s11274-019-2761-3
Mimura H (2014) Growth enhancement of the halotolerant Brevibacterium sp. JCM 6894 by methionine externally added to a chemically defined medium. Biocontrol Sci 19:151–155. https://doi.org/10.4265/bio.19.151
Mittal A, Singh G, Goyal V, Yadav A, Aneja KR, Gautam SK, Aggarwal NK (2011) Isolation and biochemical characterization of acido-thermophilic extracellular phytase producing bacterial strain for potential application in poultry feed. J Microbiol 4:273–282
Moghaieb REA, Nakamura H, Saneoka FK (2011) Evaluation of salt tolerance in ectoine-transgenic tomato plants (Lycopersicon esculentum) in terms of photosynthesis, osmotic adjustment, and carbon partitioning. GM Crops 2:58–65
Motta AS, Brandelli A (2002) Characterization of an antibacterial peptide produced by Brevibacterium linens. J Appl Microbiol 92:63–70. https://doi.org/10.1046/j.1365-2672.2002.01490.x
Mullis KB, Faloona FA (1987) Specific synthesis of DNA in vitro via apolymerase-catalyzed chain reaction. Methods Enzymol 155:335–350
Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651–681
Nawaz A, Shahbaz M, Asadullah, Imran A, Marghoob MU, Imtiaz M, Mubeen F (2020) Potential of salt tolerant PGPR in growth and yield augmentation of wheat (Triticum aestivum L.) under saline conditions. Front Microbiol 11:2019. https://doi.org/10.3389/fmicb.2020.02019
Noordman WH, Reissbrodt R, Bongers RS, Rademaker JLW, Bockelmann W, Smit G (2006) Growth stimulation of Brevibacterium sp. by siderophores. Appl Microbiol. https://doi.org/10.1111/j.1365-2672.2006.02928.x
Özyılmaz Ü, Benlioglu K (2013) Enhanced biological control of phytophthora blight of pepper by biosurfactant-producing Pseudomonas. Plant Pathol J. https://doi.org/10.5423/ppj.oa.11.2012.0176
Pankova EI, Gorokhova IN (2020) Analysis of information about the alkaline soil areas in Russia at the end of the XX and beginning of the XXI centuries. Dokuchaev Soil Bull 103:5–33. https://doi.org/10.19047/0136-1694-2020-103-5-33
Patten CL, Glick BR (1996) Bacterial biosynthesis of indole-3-acetic acid. Can J Microbiol 42:207–220. https://doi.org/10.1139/m96-032
Pei S, Niu S, Xie F, Wang W, Zhang S, Zhang G (2021) Brevibacterium limosum sp. nov., Brevibacterium pigmenatum sp. nov., and Brevibacterium atlanticum sp. nov., three novel dye decolorizing actinobacteria isolated from ocean sediments. J Microbiol 59(10):898–910. https://doi.org/10.1007/s12275-021-1235-0
Petti C, Reiber K, Ali SS, Berney M, Doohan FM (2012) Auxin as a player in the biocontrol of Fusarium head blight disease of barley and its potential as a disease control agent. BMC Plant Biol. https://doi.org/10.1186/1471-2229-12-224
Pham NP, Layec S, Dugat-Bony E et al (2017a) Comparative genomic analysis of Brevibacterium strains: insights into key genetic determinants involved in adaptation to the cheese habitat. BMC Genomics 18:955. https://doi.org/10.1186/s12864-017-4322-1
Pham NP, Layec S, Dugat-Bony E, Vidal M, Irlinger F, Monnet C (2017b) Comparative genomic analysis of Brevibacterium strains: insights into key genetic determinants involved in adaptation to the cheese habitat. BMC Genom 18:1–17. https://doi.org/10.1186/s12864-017-4322-1
Płociniczak T, Sinkkonen A, Romantschuk M, Sułowicz S, Piotrowska-Seget Z (2016) Rhizospheric bacterial strain Brevibacterium casei NH8a colonized plant tissues and enhances Cd Zn Cu phytoextraction by White Mustard. Front Plant Sci. https://doi.org/10.3389/fpls.2016.00101
Poonguzhali S, Madhaiyan M, Sa T (2008) Isolation and identification of phosphate solubilizing bacteria from chinese cabbage and their effect on growth and phosphorus utilization of plants. J Microbiol Biotechnol 18:773–777
Raho R, Paladini F, Lombardi FA, Boccarella S, Zunino B, Pollini M (2015) In-situ photo-assisted deposition of silver particles on hydrogel fibers for antibacterial applications. Mater Sci Eng C-Materials for Biol Appl 55:42–49. https://doi.org/10.1016/j.msec.2015.05.050
Ruan S, Xue Q, Tylhowska K (2002) the influence of priming on germination of rice (Oryza sativa L.) seeds and seedlings emergenceand performance in flooded soils. Seed Sci Technol 30:61–67
Sah S, Krishnani S, Singh R (2021) Pseudomonas mediated nutritional and growth promotional activities for sustainable food security. Curr Res Microb Sci. https://doi.org/10.1016/j.crmicr.2021.100084
Saleh O, Gust B, Boll B, Fiedler HP, Heide L (2009) Aromatic prenylation in phenazine biosynthesis: dihydrophenazine-1-carboxylate dimethylallyltransferase from Streptomyces anulatus. J Biol Chem 284:14439–14447
Saravanakumar D, Samiyappan R (2007) ACC deaminase from Pseudomonas fluorescens mediated saline resistance in groundnut (Arachis hypogea) plants. J Appl Microbiol 102:1283–1292. https://doi.org/10.1111/j.1365-2672.2006.03179.x
Sayyed RZ, Chincholkar SB, Reddy MS, Gangurde NS, Patel PR (2012) Siderophore producing PGPR for crop nutrition and phytopathogen suppression. In: Bacteria in agrobiology: disease management, pp 449–471
Shabani L, Sabzalian MR (2016) Arbuscular mycorrhiza affects nickel translocation and expression of ABC transporter and metallothionein genes in Festuca arundinacea. Mycorrhiza 26:67–76. https://doi.org/10.1007/s00572-015-0647-2
Shao J, Li S, Zhang N, Cui X, Zhou X, Zhang G, Shen Q, Zhang R (2015) Analysis and cloning of the synthetic pathway of the phytohormone indole-3-acetic acid in the plant-beneficial Bacillus amyloliquefaciens SQR9. Microb Cell Fact. https://doi.org/10.1186/s12934-015-0323-4
Sharma S, Kulkarni J, Jha B (2016) Halotolerant rhizobacteria promote growth and enhance salinity tolerance in peanut. Front Microbiol. https://doi.org/10.3389/fmicb.2016.01600
Shields P, Cathcart L (2010) Oxidase test protocol. American Society for Microbiology, pp 1–9
Siddikee MA, Chauhan PS, Anandham R, Han GH, Sa T (2010) Isolation, characterization, and use for plant growth promotion under salt stress, of ACC deaminase-producing halotolerant bacteria derived from coastal soil. J Microbiol Biotechnol 20:1577–1584. https://doi.org/10.4014/jmb.1007.07011
Siddikee MA, Glick BR, Chauhan PS, Yim W, Sa T (2011) Enhancement of growth and salt tolerance of red pepper seedlings (Capsicum annuum L.) by regulating stress ethylene synthesis with halotolerant bacteria containing 1-aminocyclopropane-1-carboxylic acid deaminase activity. Plant Physiol Biochem 49:427–434. https://doi.org/10.1016/j.plaphy.2011.01.015
Singh RK, Kumar DP, Singh P, Solanki MK, Srivastava S, Kashyap PL, Kumar S, Srivastava AK, Singhal PK, Arora DK (2014) Multifarious plant growth promoting characteristics of chickpea rhizosphere associated Bacilli help to suppress soil-borne pathogens. Plant Growth Regul 73:91–101
Subiramani S, Ramalingam S, Muthu T, Nile SH, Venkidasamy B (2020) Development of abiotic stress tolerance in crops by plant growth-promoting rhizobacteria (PGPR). In: Kumar M, Etesami H, Kumar V (eds) Phyto-microbiome in stress regulation. Springer, Cham, pp 125–145. https://doi.org/10.1007/978-981-15-2576-6_8
Sullivan MJ, Petty NK, Beatson SA (2011) Easyfig: a genome comparison visualizer. Bioinformatics 27:1009–1010. https://doi.org/10.1093/bioinformatics/btr039
Talaat NB (2015) Effective microorganisms improve growth performance and modulate the ROS-scavenging system in common bean (Phaseolus vulgaris L.) plants exposed to salinity stress. J Plant Growth Regul 34:35–46. https://doi.org/10.1007/s00344-014-9440-2
Tang SK, Wang Y, Schumann P, Stackebrandt E, Lou K, Jiang CL, Xu LH, Li WJ (2008) Brevibacterium album sp. nov., a novel actinobacterium isolated from a saline soil in China. Int J Syst Evol Microbiol 58:574–577. https://doi.org/10.1099/ijs.0.65183-0
Tenea GN, Ortega C (2021) Genome characterization of Lactiplantibacillus plantarum strain UTNGt2 originated from Theobroma grandiflorum (White Cacao) of Ecuadorian Amazon: antimicrobial peptides from safety to potential applications. Antibiotics. https://doi.org/10.3390/antibiotics10040383
Thawai C, Kittiwongwattana C, Thanaboripat D, Laosinwattana C, Koohakan P, Parinthawong N (2016) Micromonospora soli sp. nov., isolated from rice rhizosphere soil. Antonie Van Leeuwenhoek 109:449–456. https://doi.org/10.1007/s10482-016-0651-3
Valdés-Stauber N, Scherer S (1994) Isolation and characterization of Linocin M18, a bacteriocin produced by Brevibacterium linens. Appl Environ Microbiol 60:3809–3814. https://doi.org/10.1128/aem.60.10.3809-3814.1994
Valdes-Stauber N, Scherer S (1996) Nucleotide sequence and taxonomical distribution of the bacteriocin gene lin cloned from Brevibacterium linens M18. Appl Environ Microbiol 62:1283–1286. https://doi.org/10.1128/aem.62.4.1283-1286.1996
Verma P, Yadav AN, Khannam KS, Kumar S, Saxena AK, Suman A (2016) Molecular diversity and multifarious plant growth promoting attributes of Bacilli associated with wheat (Triticum aestivum L.) rhizosphere from six diverse agro-ecological zones of India. J Basic Microbiol 56:44–58. https://doi.org/10.1002/jobm.201500459
Vessey JK (2003) Plant growth promoting rhizobacteria as biofertilizers. Plant Soil 255:571–586
Vilela WF, Fonseca SG, Fantinatti-Garboggini F, Oliveira VM, Nitschke M (2014) Production and properties of a surface-active lipopeptide produced by a new marine Brevibacterium luteolum strain. Appl Biochem Biotechnol 174:2245–2256. https://doi.org/10.1007/s12010-014-1208-4
Wang R, Wang C, Feng Q, Liou RM, Lin YF (2021) Biological inoculant of salt-tolerant bacteria for plant growth stimulation under different saline soil conditions. J Microbiol Biotechnol 31:398–407. https://doi.org/10.4014/jmb.2009.09032
Weber T, Blin K, Duddela S, Krug D, Kim HU, Bruccoleri R (2015) antiSMASH 3.0-a comprehensive resource for the genome mining of biosynthetic gene clusters. Nucleic Acids Res 43:237–243. https://doi.org/10.1093/nar/gkv437
Wen X, Dubinsky E, Wu Y, Yu R, Chen F (2016) Wheat, maize and sunflower cropping systems selectively influence bacteria community structure and diversity in there and succeeding crop’s rhizosphere. J Integr Agric 15:1892–1902. https://doi.org/10.1016/S2095-3119(15)61147-9
Whitman W, Barnaby et al (2012) Bergey’s manual of systematic bacteriology. The actinobacteria, vol 5. Springer, New York, pp 571–575
Wright MH, Adelskov J, Greene AC (2017) Bacterial DNA extraction using individual enzymes and phenol/chloroform separation. J Microbiol Biol Educ. https://doi.org/10.1128/jmbe.v18i2.1348
Yadav AN, Verma P, Singh B, Chauahan VS, Suman A, Saxena AK (2017) Plant growth promoting bacteria: biodiversity and multifunctional attributes for sustainable agriculture. Adv Biotech Micro. https://doi.org/10.19080/aibm.2017.05.5556671
Zhao LF, Xu YJ, Ma ZQ, Deng ZS, Shan CJ, Wei GH (2013) Colonization and plant growth promoting characterization of endophytic Pseudomonas chlororaphis strain Zong1 isolated from Sophora alopecuroides root nodules Braz. J Microbiol 44:623–631. https://doi.org/10.1590/S1517-83822013000200043
Funding
This study was supported by a grant from the Russian Science Foundation [project no. 22-16-00138] and the Kazan Federal University Strategic Academic Leadership Program (PRIORITY-2030).
Author information
Authors and Affiliations
Contributions
MRS and AMM designed the research and supervised all research; MTL, GFL, DSP, and YAA carried out the experiments and analyzed the data and drafted the manuscript; MTL and GFL prepared the figures; MTL, GFL, and AMM wrote the manuscript; EIS, SGV, and AMM partook in the revision of the manuscript. All authors approved the final submitted manuscript.
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that there is no conflict of interests regarding the publication of this paper.
Supplementary Information
Below is the link to the electronic supplementary material.
13205_2022_3392_MOESM1_ESM.pdf
Supplementary file1 Phylogenetic tree showing the position of strain MG-1 relative to other strains of Brevibacterium spp. (GenBank accession numbers for all represented 16S rRNA sequences are available in Additional file 1). The phylogenetic tree is based on 16S rRNA gene alignments and was constructed with the MEGA X 10.1 software (PDF 69 KB)
13205_2022_3392_MOESM2_ESM.pdf
Supplementary file2 Heatmap depicting the pairwise comparison of Brevibacterium sp. MG-1 genome with the genomes of different Brevibacterium species (PDF 71 KB)
13205_2022_3392_MOESM3_ESM.png
Supplementary file3 The hypothetical bacteriocin gene Linocin-M18, identified using Bagel4 in the B. sediminis MG-1 genome (PNG 13 KB)
Rights and permissions
Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Lutfullin, M.T., Lutfullina, G.F., Pudova, D.S. et al. Identification, characterization, and genome sequencing of Brevibacterium sediminis MG-1 isolate with growth-promoting properties. 3 Biotech 12, 326 (2022). https://doi.org/10.1007/s13205-022-03392-z
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s13205-022-03392-z