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Complete Genome Sequence of Cellulomonas sp. JZ18, a Root Endophytic Bacterium Isolated from the Perennial Desert Tussock-Grass Panicum turgidum

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Abstract

Cellulomonas sp. JZ18 is a gram-positive, rod shaped bacterium that was previously isolated from the root endosphere of the perennial desert tussock-grass Panicum turgidum. Genome coverage of PacBio sequencing was approximately 199X. Genome assembly generated a single chromosome of 7,421,843 base pairs with a guanine-cytosine (GC) content of 75.60% with 3240 protein coding sequences, 361 pseudo genes, three ribosomal RNA operons, three non-coding RNAs and 45 transfer RNAs. Comparison of JZ18′s genome with type strains from the same genus, using digital DNA–DNA hybridization and average nucleotide identity calculations, revealed that JZ18 might potentially belong to a new species. Functional analysis revealed the presence of genes that may complement previously observed biochemical and plant phenotypes. Furthermore, the presence of a number of enzymes could be of potential use in industrial processes as biocatalysts. Genome sequencing and analysis, coupled with comparative genomics, of endophytic bacteria for their potential plant growth promoting activities under different soil conditions will accelerate the knowledge and applications of biostimulants in sustainable agriculture.

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Acknowledgements

The work presented is part of the DARWIN21 project (http://www.darwin21.org/), with the objective to improve sustainable agriculture on arid lands by exploiting microbes isolated from pioneer desert plants that are able to survive in extreme environmental conditions. The authors would thank all members of Hirt lab, CDA management team and the Bioscience Core Labs in KAUST for the technical assistance and for their help in many aspects of this work.

Funding

The work was funded by KAUST baseline research project BAS/1/1062-01-01 of H.H.

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Authors and Affiliations

  1. DARWIN21, Center for Desert Agriculture (CDA), King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia

    Abdul Aziz Eida, Heribert Hirt & Maged M. Saad

  2. Computational Bioscience Research Center (CBRC), King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia

    Salim Bougouffa & Intikhab Alam

  3. BioScience Core Lab, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia

    Salim Bougouffa

  4. Max F. Perutz Laboratories, University of Vienna, Dr. Bohrgasse 9, 1030, Vienna, Austria

    Heribert Hirt

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  1. Abdul Aziz Eida
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  2. Salim Bougouffa
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  3. Intikhab Alam
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  4. Heribert Hirt
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  5. Maged M. Saad
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Contributions

AE performed the, gDNA extraction and taxonomic analysis. SB performed genome assembly, gene prediction, annotation of genome data, IA integrated all bioinformatics databases and facilitated all genomic annotation platforms. AE, MS, and HH wrote the manuscript. MS, and HH conceived the overall study.

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Correspondence to Heribert Hirt.

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Eida, A.A., Bougouffa, S., Alam, I. et al. Complete Genome Sequence of Cellulomonas sp. JZ18, a Root Endophytic Bacterium Isolated from the Perennial Desert Tussock-Grass Panicum turgidum. Curr Microbiol 78, 1135–1141 (2021). https://doi.org/10.1007/s00284-021-02429-5

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