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Quorum Quenching Enzyme APTM01, an Acylhomoserine-Lactone Acylase from Marine Bacterium of Pseudoalteromonas tetraodonis Strain MQS005

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Abstract

Quorum sensing is a system of stimuli and response correlated to population density and involves in pathogen infection, colonization, and pathogenesis. Quorum quenching enzymes as quorum sensing inhibitors have been identified in a number of bacteria and been used to control by triggering the pathogenic phenotype. The marine bacteria of Pseudoalteromonas had wide activity of degrading AHLs as a type of signal molecule associated with quorum sensing. We screened many Pseudoalteromonas strains in large scale to explore genes of quorum quenching enzymes from the China seas by whole-genome sequencing rather than genomic library construction. Nine target strains were obtained and an acylases gene APTM01 from the strain MQS005 belonging to PvdQ type on sub-branch in phylogenetic tree. And the heterogenous host containing the vector with target gene could degrade C10-HSL, C12-HSL and OC12-HSL. The obtained AHL acylase gene would be a candidate quorum quenching gene to apply in some fields. We identified that the strains of Pseudoalteromonas have wide AHL-degrading ability depending on quorum quenching. The strains would be a resource to explore new quorum quenching enzymes.

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Acknowledgements

This work was supported by Guangdong Science and Technology Department (2013B030800001), Shenzhen Science and Technology Project (Grant Nos. JCYJ20140509174140691 and JCYJ20140417113430641), and CAS Adjunct Professorship (2013T1G0038, GJHS2014090100463583).

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Authors and Affiliations

  1. Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Beijing, People’s Republic of China

    Yonglong Pan, Yanbo Wang, Xiaoqing Yan, Chunhua Liu, Binbin Wu & Xinping He

  2. School of Life Sciences, Jilin University, Changchun, 130012, Jilin, People’s Republic of China

    Yanbo Wang

  3. University of Electronic Science and Technology of China, Chengdu, Sichuan, People’s Republic of China

    Yan Liang

  4. Zhejiang Normal University, Jinhua, Zhejiang, People’s Republic of China

    Yan Liang

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  1. Yonglong Pan
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Pan, Y., Wang, Y., Yan, X. et al. Quorum Quenching Enzyme APTM01, an Acylhomoserine-Lactone Acylase from Marine Bacterium of Pseudoalteromonas tetraodonis Strain MQS005. Curr Microbiol 76, 1387–1397 (2019). https://doi.org/10.1007/s00284-019-01739-z

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