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Potential of Pseudomonas putida PCI2 for the Protection of Tomato Plants Against Fungal Pathogens

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Abstract

Tomato is one of the most economically attractive vegetable crops due to its high yields. Diseases cause significant losses in tomato production worldwide. We carried out Polymerase Chain Reaction studies to detect the presence of genes encoding antifungal compounds in the DNA of Pseudomonas putida strain PCI2. We also used liquid chromatography-electrospray tandem mass spectrometry to detect and quantify the production of compounds that increase the resistance of plants to diseases from culture supernatants of PCI2. In addition, we investigated the presence of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase in PCI2. Finally, PCI2 was used for inoculation of tomato seeds to study its potential biocontrol activity against Fusarium oxysporum MR193. The obtained results showed that no fragments for the encoding genes of hydrogen cyanide, pyoluteorin, 2,4-diacetylphloroglucinol, pyrrolnitrin, or phenazine-1-carboxylic acid were amplified from the DNA of PCI2. On the other hand, PCI2 produced salicylic acid and jasmonic acid in Luria–Bertani medium and grew in a culture medium containing ACC as the sole nitrogen source. We observed a reduction in disease incidence from 53.33 % in the pathogen control to 30 % in tomato plants pre-inoculated with PCI2 as well as increases in shoot and root dry weights in inoculated plants, as compared to the pathogenicity control. This study suggests that inoculation of tomato seeds with P. putida PCI2 increases the resistance of plants to root rot caused by F. oxysporum and that PCI2 produces compounds that may be involved at different levels in increasing such resistance. Thus, PCI2 could represent a non-contaminating management strategy potentially applicable in vegetable crops such as tomato.

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Acknowledgments

This work was supported by grants from Secretaría de Ciencia y Técnica de la Universidad Nacional de Río Cuarto (Córdoba, Argentina), Agencia Nacional de Promoción Científica y Tecnológica (Secretaría de Ciencia y Técnica de la Nación), and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET, Argentina).

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Authors and Affiliations

  1. Departamento de Microbiología e Inmunología, Universidad Nacional de Rio Cuarto, Córdoba, Argentina

    Nicolás Pastor & Marisa Rovera

  2. Departamento de Ciencias Naturales, Universidad Nacional de Río Cuarto, Córdoba, Argentina

    Oscar Masciarelli, Sonia Fischer & Virginia Luna

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  1. Nicolás Pastor
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  2. Oscar Masciarelli
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  4. Virginia Luna
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  5. Marisa Rovera
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Correspondence to Marisa Rovera.

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Pastor, N., Masciarelli, O., Fischer, S. et al. Potential of Pseudomonas putida PCI2 for the Protection of Tomato Plants Against Fungal Pathogens. Curr Microbiol 73, 346–353 (2016). https://doi.org/10.1007/s00284-016-1068-y

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