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The functions of glutathione peroxidase in ROS homeostasis and fruiting body development in Hypsizygus marmoreus

  • Applied genetics and molecular biotechnology
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Abstract

Glutathione peroxidase (GPX) is one of the most important antioxidant enzymes for maintaining reactive oxygen species (ROS) homeostasis. Although studies on fungi have suggested many important physiological functions of GPX, few studies have examined the role of this enzyme in Basidiomycetes, particularly its functions in fruiting body developmental processes. In the present study, GPX-silenced (GPxi) strains were obtained by using RNA interference. The GPxi strains of Hypsizygus marmoreus showed defects in mycelial growth and fruiting body development. In addition, the results indicated essential roles of GPX in controlling ROS homeostasis by regulating intracellular H2O2 levels, maintaining GSH/GSSG balance, and promoting antioxidant enzyme activity. Furthermore, lignocellulose enzyme activity levels were reduced and the mitochondrial phenotype and mitochondrial complex activity levels were changed in the H. marmoreus GPxi strains, possibly in response to impediments to mycelial growth and fruiting body development. These findings indicate that ROS homeostasis has a complex influence on growth, fruiting body development, GSH/GSSG balance, and carbon metabolism in H. marmoreus.

Key points

• ROS balance, energy metabolism, fruiting development.

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Acknowledgments

We thank Jing Zhao for giving some helps in performing the experiments and thank the AJE company for providing the language editing help.

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The software in this manuscript was available.

Funding

This work was funded by the National Natural Science Foundation of China (Grant No. 31601802) and the earmarked fund of Shanghai Modern Edible Fungi-Industry Technology Research System (202009).

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Authors and Affiliations

  1. National Research Center for Edible Fungi Biotechnology and Engineering, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 309 Room, No. 1000, Jinqi Road, Fengxian District, Shanghai, 201403, China

    Jinjing Zhang, Haibo Hao, Xuelan Wu, Qian Wang, Mingjie Chen, Zhiyong Feng & Hui Chen

  2. College of Life Science, Nanjing Agricultural University, No. 1, Weigang road, XuanWu District, Nanjing, 210095, China

    Zhiyong Feng

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  1. Jinjing Zhang
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  3. Xuelan Wu
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  5. Mingjie Chen
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Contributions

Jinjing Zhang, Hui Chen, Mingjie Chen, and Zhiyong Feng conceived and designed research. Jinjing Zhang, Haibo Hao, and Xuelan Wu conducted experiments. Haibo Hao and Hui Chen contributed new reagents or analytical tools. Jinjing Zhang and Qian Wang analyzed data. Jinjing Zhang wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to Hui Chen.

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Zhang, J., Hao, H., Wu, X. et al. The functions of glutathione peroxidase in ROS homeostasis and fruiting body development in Hypsizygus marmoreus. Appl Microbiol Biotechnol 104, 10555–10570 (2020). https://doi.org/10.1007/s00253-020-10981-6

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