Abstract
Plants synthesize several thousand different polyphenols of which many have the potential to aid in preventing or treating cancer, cardiovascular, and neurodegenerative diseases. However, plants usually contain complex polyphenol mixtures impeding access to individual compounds in larger quantities. In contrast, functional integration of biosynthetic plant polyphenol pathways into microorganisms allows for the production of individual polyphenols as chemically distinct compounds, which can be synthesized in large amounts and can be more easily isolated. Over the last decade, microbial synthesis of many plant polyphenols could be achieved, and along the way, many decisive bottlenecks in the endogenous microbial host metabolism as well as in the heterologous plant pathways could be identified. In this review, we present recent advancements in metabolic engineering of microorganisms for the production of plant polyphenols and discuss how current challenges could be addressed in the future.
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Acknowledgements
We would like to thank the European Union’s Seventh Framework Program (“BacHBerry” BACterial Hosts for production of Bioactive phenolics from bERRY fruits; Project No. FP7-613793, www.bachberry.eu).
Funding
We would like to thank the German Federal Ministry of Education and Research, BMBF (“BioLiSy” Bioeconomic Lignan Synthesis; Grant No. 031A554) for financial support.
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Milke, L., Aschenbrenner, J., Marienhagen, J. et al. Production of plant-derived polyphenols in microorganisms: current state and perspectives. Appl Microbiol Biotechnol 102, 1575–1585 (2018). https://doi.org/10.1007/s00253-018-8747-5
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DOI: https://doi.org/10.1007/s00253-018-8747-5