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Characterization and application of endogenous phase-dependent promoters in Bacillus subtilis

  • Applied genetics and molecular biotechnology
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Abstract

Bacillus subtilis as an important host has been widely used in synthetic biology, metabolic engineering, and production of industrial enzymes. To fully take advantage of this organism, 114 endogenous putative promoters were measured with a green fluorescent protein reporter and four classes of phase-dependent promoters (class I: exponential phase; class II: middle-log and early stationary phases; class III: lag-log and stationary phases; class IV: stationary phase) with different strengths were identified. The transcriptional strengths ranged from 0.03 to 2.03-fold of that of the commonly used strong promoter P43. On this basis, the temperature (for instance P bltD , P ydaD , and P gerBC ) and pH (such as P abrB , P ydjO , and P opuE ) inducible phase-dependent promoters were further identified and characterized. In comparison, P abrB (class I), P spoVG (class II), and P lytR (class III) achieved the highest expression levels of esterase, keratinase, and alkaline polygalacturonate lyase, respectively. The constructed phase-dependent promoter library should have great application potentials for enzyme production, metabolic engineering, and synthetic biology.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (31670092), the Natural Science Foundation of Jiangsu Province (BK20141107), a grant from the Key Technologies R&D Program of Jiangsu Province, China, (BE2014607), and Program for Changjiang Scholars and Innovative Research Team in University (No. IRT_15R26).

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  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China

    Sen Yang, Guocheng Du, Jian Chen & Zhen Kang

  2. Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu, 214122, China

    Guocheng Du, Jian Chen & Zhen Kang

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  1. Sen Yang
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  2. Guocheng Du
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  3. Jian Chen
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Correspondence to Jian Chen or Zhen Kang.

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Yang, S., Du, G., Chen, J. et al. Characterization and application of endogenous phase-dependent promoters in Bacillus subtilis . Appl Microbiol Biotechnol 101, 4151–4161 (2017). https://doi.org/10.1007/s00253-017-8142-7

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