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Surface display on lactic acid bacteria without genetic modification: strategies and applications

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Abstract

Microbial cell surface display has attracted greater attention than ever and has numerous potential applications in biotechnology. With the safety and probiotic properties, lactic acid bacteria (LAB) have been used widely in food and industrial applications. In order to circumvent using genetically modified microorganisms which face low public acceptance and severe regulatory scrutiny, surface-engineered LAB without genetical modification are more preferred. According to the way used to obtain the fusion protein containing the passenger molecule and anchoring domain, the genetic or chemical approaches can be used to construct these surface-engineered LAB. In addition to the viable wide-type LAB, non-living bacterial-like particles (BLP) can be attached by these fusion proteins added from outside. Compared to the living LAB, BLP have a higher binding capacity and less anticarrier response. Mucosal vaccines are the predominant application of these surface-engineered LAB with no genetical modification.

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Acknowledgments

This work was financially supported by the National Basic Research Program of China (973 Program, no. 2011CB504800).

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  1. State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China

    Ruifeng Mao, Dongli Wu & Yefu Wang

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Mao, R., Wu, D. & Wang, Y. Surface display on lactic acid bacteria without genetic modification: strategies and applications. Appl Microbiol Biotechnol 100, 9407–9421 (2016). https://doi.org/10.1007/s00253-016-7842-8

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