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Protection against human papillomavirus type 16-induced tumors in mice using non-genetically modified lactic acid bacteria displaying E7 antigen at its surface

  • Applied genetics and molecular biotechnology
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Applied Microbiology and Biotechnology Aims and scope Submit manuscript

An Erratum to this article was published on 08 January 2013

Abstract

Human papillomavirus (HPV) is the causative agent of cervical cancer (CxCa) and the most commonly sexually transmitted pathogen worldwide. HPV type 16 (HPV-16) E7 oncoprotein is constitutively produced in CxCa and considered as a good antigen candidate for the development of new therapeutic CxCa vaccines. Here, we report the use of non-genetically modified, E7-expressing lactic acid bacteria (LAB) by using the cell-binding domain from Lactobacillus casei A2 phage lysin as a cell wall anchor. The versatility of this system was validated by investigating E7 stability at the surface of Lactococcus lactis and L. casei, two major species of LAB. Moreover, we demonstrated the successful use of these LAB displaying E7 antigen as a mucosal live vaccine in mice. Altogether, these results show the feasibility of using non-genetically modified LAB for low-cost mucosal immunotherapy against HPV-related CxCa in humans.

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Acknowledgments

The authors would like to thank Christine Longin (Unité UR1196 Génomique et Physiologie de la Lactation, INRA, Plateau de Microscopie Electronique—plate forme MIMA2-78352 Jouy-en-Josas cedex, France) for her precious help in immuno-electron microscopy experiments. We declare no conflict of interest.

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Authors and Affiliations

  1. Commensal and Probiotics–Host Interactions Laboratory, INRA, UMR 1319 Micalis, 78350, Jouy-en-Josas, France

    Pedro Ribelles, Bouasria Benbouziane, Philippe Langella & Luis G. Bermúdez-Humarán

  2. AgroParisTech, UMR Micalis, 78350, Jouy-en-Josas, France

    Pedro Ribelles, Bouasria Benbouziane, Philippe Langella & Luis G. Bermúdez-Humarán

  3. Instituto de Productos Lácteos de Asturias (CSIC), Villaviciosa, Spain

    Juan E. Suárez

  4. Area de Microbiología, Instituto Universitario de Biotecnología, Universidad de Oviedo, Oviedo, Spain

    Pedro Ribelles & Juan E. Suárez

  5. Laboratory of Beneficial Microorganisms, Functional Foods and Health, Department of Biotechnology, University of Mostaganem, Lahcen Street, P.O. Box 300, Mostaganem, 27000, Algeria

    Bouasria Benbouziane & Ali Riazi

Authors
  1. Pedro Ribelles
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  2. Bouasria Benbouziane
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  3. Philippe Langella
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  4. Juan E. Suárez
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  5. Luis G. Bermúdez-Humarán
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  6. Ali Riazi
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Corresponding author

Correspondence to Luis G. Bermúdez-Humarán.

Additional information

Pedro Ribelles and Bouasria Benbouziane equally contributed to this paper.

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Ribelles, P., Benbouziane, B., Langella, P. et al. Protection against human papillomavirus type 16-induced tumors in mice using non-genetically modified lactic acid bacteria displaying E7 antigen at its surface. Appl Microbiol Biotechnol 97, 1231–1239 (2013). https://doi.org/10.1007/s00253-012-4575-1

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  • DOI: https://doi.org/10.1007/s00253-012-4575-1

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