Abstract
Biofilm-based algal cultivation has received increased attention as a potential platform for algal production and other applications such as wastewater treatment. Algal biofilm cultivation systems represent an alternative to the suspension-based systems that have yet to become economically viable. One major advantage of algal biofilm systems is that algae can be simply harvested through scraping and thus avoid the expensive harvesting procedures used in suspension-based harvesting such as flocculation and centrifugation. In recent years, an assortment of algal biofilm systems have been developed with various design configurations and biomass production capacities. This review summarizes the state of the art of different algal biofilm systems in terms of their design and operation. Perspectives for future research needs are also discussed to provide guidance for further development of these unique cultivation systems.
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References
Barros A, Goncalves A, Simoes M, Pires J (2015) Harvesting techniques applied to microalgae: a review. Renew Sustain Energy Rev 41:1489–1500
Bernstein H, Kesaano M, Moll K, Smith T, Gerlach R, Carlson R, Miller C, Peyton B, Cooksey K, Gardner R, Sims R (2014) Direct measurement and characterization of active photosynthesis zones inside wastewater remediating and biofuel producing microalgal biofilms. Bioresour Technol 156:206–215
Bioprocess H2O LLC (2014) Systems, apparatuses and methods for treating wastewater. Patent US8809037 B2. 19 Aug 2014
Blanken W, Janssen M, Cuaresma M, Libor Z, Bhaiji T, Wiffels R (2014) Biofilm growth of Chlorella sorokiniana in a rotating biological contactor based photobioreactor. Biotechnol Bioeng 111:2436–2445
Boelee N, Janssen M, Temmink H, Shrestha R, Buisman C, Wijffels R (2014) Nutrient removal and biomass production in an outdoor pilot-scale phototrophic biofilm reactor for effluent polishing. Appl Biochem Biotechnol 172:405–422
Brune D, Lundquist T, Benemann J (2009) Microalgal biomass for greenhouse gas reductions: potential for replacement of fossil fuels and animal feeds. J Environ Eng 135:1136–1144
Cao J, Yuan W, Pei Z, Davis T, Cui Y, Beltran M (2009) A preliminary study of the effect of surface texture on algae cell attachment for a mechanical-biological energy manufacturing system. J Manuf Sci Eng 131:645051–645053
Cheng P, Wang J, Liu T (2014) Effects of nitrogen source and nitrogen supply model on the growth and hydrocarbon accumulation of immobilized biofilm cultivation of B. braunii. Bioresour Technol 166:527–533
Christenson L, Sims R (2012) Rotating algal biofilm reactor and spool harvester for wastewater treatment with biofuels by-products. Biotechnol Bioeng 109:1674–1684
Cui Y, Yuan W, Cao J (2014) Effect of surface texturing on microalgal cell attachment to solid carriers. Int J Agric Biol Eng 7:82–91
Dassey A, Theegala C (2013) Harvesting economics and strategies using centrifugation for cost effective separation of microalgae cells for biodiesel applications. Bioresour Technol 128:241–245
Dau H, Zaharieva I (2009) Principles, efficiency, and blueprint character of solar-energy conversion in photosynthetic water oxidation. Acc Chem Res 42:1861–1870
Davis R, Aden A, Pienkos P (2011) Techno-economic analysis of autotrophic microalgae for fuel production. Appl Energy 88:3524–3531
Fitch M, England E (2002) Biological fixed film systems. Water Environ Res 74:1–87
Gao F, Yang Z, Li C, Zeng G, Ma D, Zhou L (2015) A novel algal biofilm membrane photobioreactor for attached microalgae growth and nutrients removal from secondary effluent. Bioresour Technol 179:8–12
Genin S, Aitchison S, Allen D (2013) Design of algal film photobioreactors: material surface energy effects on algal film productivity, colonization and lipid content. Bioresour Technol 155:136–143
Gerde J, Yao L, Lio J, Wen Z, Wang T (2014) Microalgae flocculation: impact of flocculant type, algae species and cell concentration. Algal Res 3:30–35
Godos I, Gonzalez C, Becares E, Garcia-Encina P, Munoz R (2008) Simultaneous nutrients and carbon removal during pretreated swine slurry degradation in a tubular biofilm photobioreactor. Environ Biotechnol 82:187–194
Gross M, Wen Z (2014) Yearlong evaluation of performance and durability of a pilot-scale revolving algal biofilm (RAB) cultivation system. Bioresour Technol 171:50–58
Gross M, Henry W, Michael C, Wen Z (2013) Development of a rotating algal biofilm growth system for attached microalgae growth with in situ biomass harvest. Bioresour Technol 150:195–201
Gross M, Mascarenhas V, Wen Z (2015) Evaluating algal growth performance and water use efficiency of pilot-scale revolving algal biofilm (RAB) culture systems. Biotechnol Bioeng. in press
Gullicks H, Hasan H, Das D, Moretti C, Hung Y (2011) Biofilm fixed film systems. Water 3:843–868
Guzzon A, Bohn A, Diociaiutic M, Albertano P (2008) Cultured phototrophic biofilms for phosphorous removal in wastewater treatment. Water Res 42:4357–4367
Hassard F, Biddle J, Carmell E, Jefferson B, Tyrell S, Stephenson T (2015) Rotating biological contactors for wastewater treatment—a review. Process Saf Environ Protect 94:285–306
He S, Xue G (2010) Algal-based immobilization process to treat the effluent from a secondary wastewater treatment plant (WWTP). J Hazard Mater 178:895–899
Hill R, Fanta S, Roberts B (2009) Quantifying phosphorus and light effects in stream algae. Limnol Oceanogr 54:368–380
Irving T, Allen D (2011) Species and material considerations in the formation and development of microalgal biofilms. Appl Microbiol Biotechnol 92:283–294
Johnson M, Wen Z (2010) Development of an attached microalgal growth system for biofuel production. Appl Microbiol Biotechnol 85:525–534
Kang J, Wang T, Xin H, Wen Z (2014) A laboratory study of mitigating ammonia gas emission from animal production operations using microalgae. J Air Waste Manag Assoc 64:330–339
Katarzyna L, Sai G, Singh O (2015) Non-enclosure methods for non-suspended microalgae cultivation: literature review and research needs. Renew Sust Energ Rev 14:1418–1427
Kesaano M, Sims R (2014) Algal biofilm based technology for wastewater treatment. Algal Res 5:231–240
Kesaano M, Gardner R, Moll K, Lauchnor E, Gerlach R, Petron B, Sims R (2015) Dissolved inorganic carbon enhanced growth, nutrient uptake, and lipid accumulation in wastewater grown microalgal biofilms. Bioresour Technol 180:7–15
Kibede-Westhead E, Pizarro C, Mulbry W, Wilkie A (2003) Production and nutrient removal by periphyton grown under different loading rates of anaerobically digested flushed dairy manure. J Phycol 39:1275–1282
Kohler J, Hansen P, Wahl M (1999) Colonization patterns at the substratum-water interface: how does surface microtopography influence recruitment patterns of sessile organisms? Biofouling 14:237–248
Lin Y, Leu J, Lan C, Lin P, Chang F (2003) Kinetics of inorganic carbon utilization by microalgal biofilm in a flat plate photobioreactor. Chemosphere 53:779–787
Liu T, Wang J, Hu Q, Cheng P, Ji B, Liu J, Chen Y, Zhang W, Chen X, Chen L, Gao L, Ji C, Wang H (2013) Attached cultivation technology of microalgae for efficient biomass feedstock production. Bioresour Technol 127:216–222
Mata T, Martins A, Caetanao N (2010) Microalgae for biodiesel production and other applications: a review. Renew Sust Energ Rev 14:217–232
Michael C, del Ninno M, Gross M, Wen Z (2015) Use of wavelength-selective optical light filters for enhanced microalgal growth in different algal cultivation systems. Bioresour Technol 179:473–482
Mulbry W, Wilkie A (2001) Growth of benthic freshwater algae on dairy manures. J Appl Phycol 13:301–306
Mulbry W, Kondrad S, Pizarro C, Kibede-Westhead E (2008) Treatment of dairy manure effluent using freshwater algae: algal productivity and recovery of manure nutrients using pilot-scale algal turf scrubbers. Bioresour Technol 99:8137–8142
Naumann T, Çebi Z, Podola B, Melkonian M (2013) Growing microalgae as aquaculture feeds on twin-layers: a novel solid-state photobioreactor. J Appl Phycol 25:1413–1420
Orandi S, Lewis D, Moheimani N (2012) Biofilm establishment and heavy metal removal capacity of an indigenous mining algal microbial consortium in a photo-rotating biological contactor. J Ind Microbiol Biotechnol 39:1321–1331
Ozkan A, Berberoglu H (2013) Cell to substratum and cell to cell interactions of microalgae. Colloids Surf B: Biointerfaces 112:302–309
Ozkan A, Kinney K, Katz L, Berberoglu H (2012) Reduction of water and energy requirement of algae cultivation using an algae biofilm photobioreactor. Bioresour Technol 114:542–548
Palmer J, Flint S, Brooks J (2007) Bacterial cell attachment, the beginning of a biofilm. J Ind Microbiol Biotechnol 34:577–588
Pittman J, Dean A, Osundeko O (2011) The potential of sustainable algal biofuel production using wastewater resources. Bioresour Technol 102:17–25
Pohlon E, Marxsen J, Kusel K (2009) Pioneering bacterial and algal communities and potential extracellular enzyme activities of stream biofilms. FEMS Microb Ecol 71:364–373
Posadas E, Garcia-Encina P, Soltau A, Dominguez A, Diaz I, Munoz R (2013) Carbon and nutrient removal from centrates and domestic wastewater using algal–bacterial biofilm bioreactors. Bioresour Technol 139:50–58
Przytocka-Jusiak M, Baszczyk M, Kosinska E, Bisz-Konarzewska A (1984) Removal of nitrogen from industrial wastewaters with the use of algal rotating disks and denitrification packed bed reactor. Water Res 18:1077–1082
Schnurr P, Espie G, Allen G (2013) Algae biofilm growth and the potential to stimulate lipid accumulation through nutrient starvation. Bioresour Technol 136:337–344
Schultze L, Simon M, Li T, Langenbach D, Podola B, Melkonian M (2015) High light and carbon dioxide optimize surface productivity in a Twin-Layer biofilm photobioreactor. Algal Res 8:37–44
Sekar R, Venugopalan V, Satpathy K, Nair K, Rao V (2004) Laboratory studies on adhesion of microalgae to hard substrates. Hydrobiologia 512:109–116
Shi J, Podola B, Meklonian M (2014) Application of a prototype-scale Twin-Layer photobioreactor for effective N and P removal from different process stages of municipal wastewater by immobilized microalgae. Bioresour Technol 154:260–266
Spengel D, Dzombak D (1992) Biokinetic modeling and scale-up considerations for rotating biological contactors. Water Environ Res 64:223–235
Tian Y, Zheng L, Sun D (2006) Functions and behaviors of activated sludge extracellular polymeric substances (EPS): a promising environmental interest. J Environ Sci 18:420–427
Tredici MR (2010) Photobiology of microalgae mass cultures: understanding the tools for the next green revolution. Biofuels 1:143–162
Zamalloa C, Boon N, Verstraete W (2013) Decentralized two-stage sewage treatment by chemical-biological flocculation combined with microalgae biofilm for nutrient immobilization in a roof installed parallel plate reactor. Bioresour Technol 130:152–160
Zuang L, Hu H, Wu Y, Wang T, Zhang T (2014) A novel suspended-solid phase photobioreactor to improve biomass production and separation of microalgae. Bioresour Technol 153:399–402
Conflict of interest
Authors Z. Wen, M. Gross, and D. Jarboe have equity interests and management roles in Gross-Wen Technologies, LLC. The terms of this arrangement have been reviewed and approved by Iowa State University in accordance with its conflict of interest policies.
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Gross, M., Jarboe, D. & Wen, Z. Biofilm-based algal cultivation systems. Appl Microbiol Biotechnol 99, 5781–5789 (2015). https://doi.org/10.1007/s00253-015-6736-5
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DOI: https://doi.org/10.1007/s00253-015-6736-5